Cyclin D1 protein stability is regulated by ubiquitin-proteasome mechanism mediated by multiple E3 ligases, including newly identified Keap1, DDB2, and WSB2.
A strategy to identify high-quality commercially available antibodies for research reveals extensive use of non-specific antibodies and offers solutions for future large-scale testing.
Cancer-directed treatment using Tumor-Treating Fields spatially modulates the tumor microenvironment at the cellular and molecular levels, decreasing nanotube-based cellular networks of communication while creating a microenvironment more susceptible to immunotherapeutic strategies.
Alex W Chan, Malgorzata Broncel ... Sebastian Lourido
Comprehensive characterization of kinase-dependent phosphorylation in the human pathogen Toxoplasma gondii identifies a trafficking adaptor complex required for virulence factor secretion and infection.
Abigail C Neininger-Castro, James B Hayes ... Dylan Tyler Burnette
Software is introduced for the rapid and comprehensive quantification of sarcomere-containing myofibrils and their precursors in cardiac myocytes, aiding in the advancement of cardiovascular cell biology investigations.
Disruption of the intracellular vimentin network upon cellular stress results in enhanced cell surface vimentin on human osteoarthritic chondrocytes and might represent a novel membrane-associated marker of stress-induced premature senescence.
Maruthi Kumar Pabba, Christian Ritter ... M Cristina Cardoso
Live cell tracking of labeled DNA reveals that replisome loading rather than DNA synthesis decreases the chromatin motion during S-phase in human cells.
Acetate concentration, which is significantly increased in association with energy stresses such as those that occur with diabetes or starvation, is emerging as a novel 'ketone body' with potential as a parameter for evaluating the progression of energy stress.
Elliott L Paine, Jack J Skalicky ... Wesley I Sundquist
Biochemical, structural, imaging, and functional studies reveal how the ESCRT-III protein IST1 recruits the CAPN7 cysteine protease to midbodies, where its proteolytic activity is required for efficient abscission and NoCut checkpoint arrest.
