Minimally disruptive genetic code expansion enables faithful, site-specific labeling of G3BP1 and TDP-43, revealing native stress granule dynamics and ALS-linked protein behavior in live cells.
Proline hydroxylation of RepoMan (CDCA2) at P604 by PHD1 is shown to be important for mitotic progression and regulating interaction with PP2A regulatory subunit B56 gamma.
A robust workflow for the identification of proline hydroxylation sites in proteins was established, using hydrophilic interaction chromatography enrichment and LC-MS/MS together with refining and filtering parameters during data analysis.
