Genetic experiments integrated with ChIP-sequencing and CRISPR interference reveal that overexpression of Foxc1 transcriptionally activates the expression of Arhgap36 and dysregulates multiple facets of Hedgehog pathway activity.
Elin Larsson, Aleksei Kabedev ... Richard Lundmark
Membrane insertion of Dyngo-4a results in a dynamin-independent inhibition of caveola dynamics, revealing that outer leaflet lipid packing in the plasma membrane contributes to the confinement of caveolae.
Gaseous CO2 acts as a neuron to glia messenger in peripheral myelin to trigger opening of Schwann Cell Cx32 hemichannels in response to axonal action potential propagation.
Minimally disruptive genetic code expansion enables faithful, site-specific labeling of G3BP1 and TDP-43, revealing native stress granule dynamics and ALS-linked protein behavior in live cells.
Proline hydroxylation of RepoMan (CDCA2) at P604 by PHD1 is shown to be important for mitotic progression and regulating interaction with PP2A regulatory subunit B56 gamma.
A robust workflow for the identification of proline hydroxylation sites in proteins was established, using hydrophilic interaction chromatography enrichment and LC-MS/MS together with refining and filtering parameters during data analysis.
