Katherine G Zyner, Darcie S Mulhearn ... Shankar Balasubramanian
For the first time, the spectrum of genes and pathways interacting with alternative DNA structures called G-quadruplexes in humans is revealed highlighting new possibilities for the pursuit of cancer therapies.
Erick E Nakagaki-Silva, Clare Gooding ... Christopher WJ Smith
By focusing on RNA-binding proteins whose genes have super enhancers in smooth muscle cells, the protein RBPMS was identified as an alternative splicing master regulator.
Johann Holzmann, Antonio Z Politi ... Jan-Michael Peters
Absolute quantification of cohesin, CTCF, NIPBL, WAPL and sororin in HeLa cells implies that some genomic cohesin and CTCF enrichment sites are unoccupied at any one time.
Michael P Meers, Terri D Bryson ... Steven Henikoff
Enhancements to the CUT&RUN antibody-targeted nuclease strategy for chromatin profiling include an improved affinity-cleavage fusion protein and digestion protocol, and a simplified calibration strategy.
Transposon activation during male gametogenesis is caused by interactions between DNA demethylation and linker histone H1, developmental depletion of which promotes pollen fertility.
Previously uncharacterized long repeat sequences are associated with significant genome variation that can increase fitness and promote antifungal drug resistance in diverse isolates of Candida albicans.
Madhusudhan Srinivasan, Naomi J Petela ... Kim A Nasmyth
In G1 cells, Scc2 loads and maintains cohesin on chromosomes by counteracting a Wapl-independent releasing activity, which is neutralized in S phase by CDK1.
The sharp expression pattern driven by a classic, simple animal enhancer is determined by multiple molecular mechanisms, not only cooperative binding of the activating transcription factor as was previously thought.
