A high-fidelity and efficient strategy to use CRISPR/Cas9 to reversibly insert large DNA fragments into somatic cells to label or modify endogenous proteins for research and, in the future, for gene therapy.
The direction-selective circuit in the retina adjusts the contributions of excitatory and inhibitory mechanisms under different stimulus conditions to generate context-dependent neural representations of visual features.
Eljo Van Battum, Celine Heitz-Marchaland ... Alain Chédotal
3D imaging and time-lapse video-microscopy show that the PlexinB2 receptor controls the tempo of granule cell differentiation and sets the pace of their migration in mouse cerebellum.
Mingzheng Wu, Samuel Minkowicz ... Yevgenia Kozorovitskiy
Interconnected ventral tegmental area and medial prefrontal cortex circuits causally link dopamine dynamics, aversive learning, and the effects of a promising rapidly acting antidepressant ketamine.
Ketamine strengthens connections between two brain regions that are involved in the production and regulation of dopamine, which may explain how the drug can alleviate depression.
Kv1.1 potassium channels regulate postnatal neurogenesis in the hippocampus via increasing the membrane excitability that activates the TrkB signaling pathway in a cell-autonomous manner.
Visual input to prefrontal cortex preferentially targets neurons with both sensory and motor properties, and the synaptic efficacy of these inputs is facilitated by working memory.
Jessica Douthit, Ariel Hairston ... Jessica E Treisman
R7 photoreceptors require the endosomal protein Lost and Found to form stable connections only when this protein is also present in neurons that are synaptic partners of R7.
Different Purkinje cell subpopulations show distinct developmental profiles of physiological activity, climbing fiber inputs and axonal and dendritic morphology, matching different timelines of cerebellum-dependent behaviors.
Contrasting with neural network theories, a study of the cross-species perception of dynamic faces with highly realistic human and monkey avatars reveals independent perceptual encoding of facial shape and expression.
