A specific role for Importin-5 and NASP in the import and nuclear hand-off of monomeric H3

  1. Alonso Javier Pardal
  2. Andrew James Bowman  Is a corresponding author
  1. University of Warwick, United Kingdom

Abstract

Core histones package chromosomal DNA and regulate genomic transactions, with their nuclear import and deposition involving importin- proteins and a dedicated repertoire of histone chaperones. Previously, a histone H3-H4 dimer has been isolated bound to Importin-4 (Imp4) and the chaperone ASF1, suggesting H3 and H4 fold together in the cytoplasm before nuclear import. However, other studies have shown the existence of monomeric H3 in the nucleus, indicating a post import folding pathway. Here we report that the predominant importin associated with cytoplasmic H3 is Importin-5 (Imp5), which hands off its monomeric cargo to nuclear sNASP. Imp5, in contrast to Imp4, binds to both H3 and H4 containing constitutively monomeric mutations and binds to newly synthesised, monomeric H3 tethered in the cytoplasm. Constitutively monomeric H3 retains its interaction with NASP, whereas monomeric H4 retains interactions specifically with HAT1 and RBBP7. High-resolution separation of NASP interactors shows the 's' isoform but not the 't' isoform associates with monomeric H3, whilst both isoforms associate with H3-H4 dimers in at least three discrete multi-chaperoning complexes. In vitro binding experiments show mutual exclusivity between sNASP and Imp5 in binding H3, suggesting direct competition for interaction sites, with the GTP-bound form of Ran required for histone transfer. Finally, using pulse-chase analysis, we show that cytoplasm tethered histones do not interact with endogenous NASP until they reach the nucleus, whereupon they bind rapidly. We propose an Imp5-specific import pathway for monomeric H3 that hands off to sNASP in the nucleus, with a parallel H4 pathway involving Imp5 and the HAT1-RBBP7 complex, followed by nuclear folding and hand-off to deposition factors.

Data availability

Mass spectrometry proteomics raw data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029354 and 10.6019/PXD029354. Supplementary Table S4 contains the analysed proteomic results.Uncropped, source images for western blots and gels are provided in the supplementary material.

The following data sets were generated

Article and author information

Author details

  1. Alonso Javier Pardal

    Division of Biomedical Sciences, University of Warwick, Coventry, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  2. Andrew James Bowman

    Division of Biomedical Sciences, University of Warwick, Coventry, United Kingdom
    For correspondence
    a.bowman.1@warwick.ac.uk
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5507-669X

Funding

Wellcome Trust (208801/Z/17/Z)

  • Alonso Javier Pardal
  • Andrew James Bowman

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Jerry L Workman, Stowers Institute for Medical Research, United States

Publication history

  1. Preprint posted: October 20, 2021 (view preprint)
  2. Received: July 11, 2022
  3. Accepted: September 4, 2022
  4. Accepted Manuscript published: September 6, 2022 (version 1)

Copyright

© 2022, Pardal & Bowman

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Alonso Javier Pardal
  2. Andrew James Bowman
(2022)
A specific role for Importin-5 and NASP in the import and nuclear hand-off of monomeric H3
eLife 11:e81755.
https://doi.org/10.7554/eLife.81755

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