Franka H van der Linden, Stephen C Thornquist ... Joachim Goedhart
Directed evolution was used to engineer a green fluorescent biosensor for quantitative calcium imaging that remains fluorescent, yet shows a large change in lifetime in cells.
Deletion of specific herpes simplex virus proteins prevents the progression of virus assembly at discrete stages, allowing detailed 3D imaging of rare events in the morphogenesis of virus particles.
Testosterone promotes the conversion of glucose to fatty acids by increasing the expression of ACLY in seminal vesicle epithelial cells, thereby regulating the seminal plasma components.
Extracellular adenosine deamination by ADGF generates ammonia that directs tip organizer development, revealing a conserved metabolic mechanism linking nucleotide turnover to developmental regulation.
Ripples maintain time-locked occurrence across the septo-temporal axis and hemispheres while showing local phase coupling, revealing a dual mode of synchrony in CA1 network dynamics.
A vessel on a chip microfluidic device recapitulates the main features of meningococcal vascular colonization, providing high time and space microscopy resolution of the infection process in 3D.
Genes steadily associated with prognosis define conserved mitotic and immune programs that robustly predict cancer patient survival and therapeutic resistance across tumor types.
Pyruvate dictates redox balance and cell size, establishing metabolism as a dominant regulator of cellular fitness, unveiling new strategies to modulate cancer, regeneration, and diabetes.
