Single-cell combined spatial transcriptomics provides the molecular foundation for investigating how ligamental cell identities, biochemical functions, and interactions contributed to the ligamental degeneration process.
Ernesto Ciabatti, Ana González-Rueda ... Marco Tripodi
SiR does not revert to wild type when produced in high-TEVp cell lines, is non-toxic in vivo, maintains transsynaptic spreading capabilities and revertant mutations do not accumulate during in vivo experiments.
Yong-Il Kim, Rebecca O'Rourke, Charles G Sagerström
Combined single nucleus RNAseq and ATACseq enabled the first molecular resolution of all hindbrain rhombomeres, the derivation of a unique gene regulatory network for each rhombomere, and the identification of gene expression changes as rhombomeres form from early progenitor pools.
Functional ultrasound imaging was used to monitor brain hemodynamics and sensory-evoked thalamocortical functions in awake rats pre- and post-stroke, advancing our understanding toward new therapeutic developments.
Low-profile tetrodes that are compatible with wide-field imaging provide the opportunity to evaluate the relationship between spiking neurons and broad networks.
Hannah Jones, Jenny A Willis ... Giorgio F Gilestro
Coccinella, an open-source framework, combines real-time tracking on microcomputers with statistical learning, optimising behaviour analysis in Drosophila melanogaster while surpassing other advanced systems in throughput and performance.
Jessica Jacobs-Li, Weiyi Tang ... Marianne E Bronner
Single-cell transcriptome analysis of sacral compared with vagal neural crest-derived cells in the developing chick enteric nervous system reveals both similarities and differences between these two cell populations.
Abigail C Neininger-Castro, James B Hayes ... Dylan Tyler Burnette
Software is introduced for the rapid and comprehensive quantification of sarcomere-containing myofibrils and their precursors in cardiac myocytes, aiding in the advancement of cardiovascular cell biology investigations.
