Alexander J Sercel, Alexander N Patananan ... Michael A Teitell
Applied pressure enables efficient isolated mitochondrial transfer and stable mitochondrial DNA (mtDNA) transplantation into a range of mtDNA-depleted cell types to restore respiration.
Computational models and software connect metagenomics to metabolic network reconstruction, assess metabolic complementarity between species, and identify critical species associated to functions of interest.
Deep and comprehensive proteomic resource of human intervertebral disc at high spatial resolution with a methodological flow reveals new insights in disc homeostasis and degeneration.
Andrew W DeVilbiss, Zhiyu Zhao ... Sean J Morrison
A method was developed for the metabolomic analysis of small numbers of flow cytometrically isolated cells from rare cell populations such as hematopoietic stem cells and circulating cancer cells.
The axial organization and dynamics of the HOPS complex at membrane surface are resolved by graphene-induced energy transfer with subnanometer resolution.
A new imaging modality is described that can simultaneously record from several dishes without using robotics, which enables researchers to perform high-throughput, continuous measurements on biological samples.
A flexible and accurate methodology to fit reduced compartmental models to dendritic morphologies shows that models with very few compartments can capture many dendritic computations.
Julio D Perez, Susanne tom Dieck ... Erin M Schuman
Single somata and dendrites of individual rat neurons were laser-captured and sequenced to discover and compare the subcellular transcriptomes, identifying over 4000 mRNAs in GABAergic dendrites.
A new nomenclature convention for mammalian cell types utilizing shared taxonomies and quantitative cell features is applied to high-quality, open data from the mouse and human brain.
