Daniel A Gehrlach, Caroline Weiand ... Nadine Gogolla
Whole-brain in- and outputs of inhibitory and excitatory neurons located in distinct subregions of the mouse insular cortex were mapped using viral-tracing strategies.
A new tool to visualize blood-feeding mosquitoes in high resolution and quantitatively characterize their behavior sheds light on contact-dependent sensing and blood-feeding dynamics of several medically relevant mosquito species.
Mikel Garcia-Marcos, Kshitij Parag-Sharma ... Lien T Nguyen
LOV2GIVe allows to activate Gi proteins non-invasively with innocuous blue light based on a design principle unrelated to light-activated GPCRs (metazoan opsins), thereby expanding the range of potential experimental applications.
Fern Sha, Ahmed S Abdelfattah ... Eric R Schreiter
Protein engineering of a reversibly switchable fluorescent protein enables post-hoc reversible and repeatable marking of elevated calcium concentrations using blue light.
Computational efforts yield a zebrafish transcriptome annotation that provides more sensitive and comprehensive gene detection over existing annotations when used for bulk or single-cell RNA-seq.
Noriko Ichino, MaKayla R Serres ... Stephen C Ekker
The analysis of the first 1000 revertible protein trap alleles in zebrafish resulted in new functional genomic annotations and produced a panel of potential new models of human disease.
Timothy P O'Leary, Kaitlin E Sullivan ... Mark S Cembrowski
Basolateral amygdala excitatory neurons are a highly heterogenous collection of neurons that spatially covary in molecular, cellular, and circuit properties.
The use of adequate statistics is demonstrated to be an essential prerequisite to derive conclusions on thermodynamics and kinetics from molecular simulations.
Simone Bersini, Roberta Schulte ... Martin W Hetzer
Direct reprogramming of smooth muscle cells from HGPS patients revealed that BMP4 is a key contributor of vascular degeneration and might represent a new therapeutic target.
Bruno Sylvain Carturan, Jason Pither ... Lael Parrott
A new agent-based model enables predicting how coral species richness and functional diversity affect the functioning and resilience of coral reef ecosystems.