A family of fluorescent biosensors for nicotinamide adenine dinucleotides allows quantification of these cofactors in live cells with spatio-temporal resolution.
A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).
Rogier B Mars, Stamatios N Sotiropoulos ... Saad Jbabdi
White matter connectivity assessed using diffusion MRI allows one to compare whole-brain organization between different animal species in a quantitative fashion, identifying homologous areas and regions of unique specialization.
Compiling public datasets into a single, centralised repository and linking directly to analytical software completely transforms the scale and scope of causal inference across the phenome.
Iwan Zimmermann, Pascal Egloff ... Markus A Seeger
Synthetic single domain antibody libraries and a binder selection cascade encompassing ribosome and phage display enable the selection of conformation-specific binders against previously intractable membrane proteins within three weeks.
Transgenic expression of glucanase, xylanase and phytase in pigs enhances growth performance and reduces nitrogen/phosphorus emission, and offers a very valuable biological strategy for sustainable resource utilization and environmental protection in the pork industry.
ChAR-seq is a massively parallelized de novo RNA mapping assay, which is capable of generating hundreds to thousands of RNA-binding maps with no a priori knowledge of target RNAs.
Pooran Singh Dewari, Benjamin Southgate ... Steven M Pollard
Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.
