Bridgette M Cumming, Kelvin W Addicott ... Adrie JC Steyn
Quantifiable bioenergetic parameters, determined from extracellular flux analyses, are distinct between macrophages infected with Mycobacteriumtuberculosis or vaccine strain M. bovis BCG, enabling assessment of future vaccine and drug efficacy.
A function-based genetic screen using the Caenorhabditis elegans axotomy model identifies new regulators and an inhibitory role for NAD+ in axon regeneration, expanding the understanding of axon injury responses and regeneration.
A new platform for imaging live Drosophila adults yields vivid movies of the midgut over prolonged time scales, opening the door to the real-time study of organ renewal dynamics in a near-native context.
A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.
Jane Anne Horne, Carlie Langille ... Ian A Meinertzhagen
FIB-SEM is used to identify comprehensively and reconstruct 192 neurons and their complete connectome for glomerulus VA1v of the Drosophila antennal lobe, in particular to reconstruct its local interneurons.
Profiling chromatin accessibility and nuclear transcription across Caenorhabditis elegans development and ageing generated the first map of transcriptional regulatory elements and their activities across an animal's life.
Pyramidal cells of the subiculum, a major output cell type of the hippocampus, can be deconstructed into distinct subtypes that exhibit a patchwork-like organization in space.
It is technically difficult to identify transport proteins, but here a method is presented that exploits the principle that they are stabilized in detergent solution when they bind their substrate.
