Alex J Noble, Venkata P Dandey ... Bridget Carragher
Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.
Willow Coyote-Maestas, David Nedrud ... Daniel Schmidt
By measuring the impacts of thousands of mutations on potassium channel trafficking and function, we illuminate the molecular basis of folding, structure–function relationships, and how these are altered in disease.
Katharina van Pee, Alexander Neuhaus ... Özkan Yildiz
The near-atomic cryoEM pore complex structure of pneumolysin, the main virulence factor of Streptococcus pneumoniae, shows how the individual domains rearrange during the pore formation.
Patrick C Hoffmann, Stefano L Giandomenico ... Wanda Kukulski
Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.
Profiling spatially defined fibroblasts in the pancreatic ductal adenocarcinoma (PDAC) tumor microenvironment identifies high levels of podoplanin and hypoxia in tumor-proximal fibroblasts associated with bad prognosis, whilst inflammatory markers define more distal fibroblast regions and associate with better outcomes.
C Keith Cassidy, Benjamin A Himes ... Peijun Zhang
An atomic model of the bacterial chemosensory array obtained through the synthesis of cryo-electron tomography and large-scale molecular-dynamics simulations reveals a new kinase conformation during signaling events.
Vincent Croset, Christoph D Treiber, Scott Waddell
Sequencing mRNA from thousands of single cells from the Drosophila brain highlights the extent of cellular diversity and reveals co-expression of specific neuropeptides with particular fast-acting neurotransmitters and monoamines.
Cryo electron microscopy and structure-based mutagenesis reveal that the bacteriophage BPP-1 contains two of the three major recognized viral folds, one of which exhibits a new topology.
A detailed description of the structure of procentriole MT triplet by cryoET, along with its associated non-tubulin proteins and its assembly intermediates, reveals possible molecular mechanism for the procentriole assembly.
Germline V-gene usage can be inferred from cryoEM reconstructions of antigen-antibody complexes to guide de novo antibody sequencing of complex mixtures by mass spectrometry.
