Human plasma contains protein-protected mRNA fragments, myriad repeat RNAs, and novel intron RNAs, including a family of structured full-length excised introns, some corresponding to mirtron pre-miRNAs and agotrons.
Transcriptome and eCLIP analyses in mouse and human reveal splicing factor proline/glutamine rich (SFPQ) as a conserved and critical guardian of long-intron integrity, splicing, and circular RNA (circRNA) production.
Viewing the dynamic interactions of individual spliceosomal subcomplexes with single pre-messenger RNA molecules reveals how nearby flanking splice sites accelerate pre-spliceosome assembly and the splicing of multi-intron pre-mRNAs.
Hundreds of cell growth and stress response genes are controlled by a rare small RNA component of an ancient splicing machinery, providing a raison d'être for its previously unexplained evolutionary conservation.
Systematic splice site mutagenesis and the identification of splicing intermediates provides evidence that the dominant mechanism for the generation of circular RNA in fission yeast proceeds through an exon-containing lariat precursor.