A systematic study of RNA localization unexpectedly finds a set of free circular introns with a non-canonical C branchpoint enriched in neuronal projections.

Biochemical, transcriptomic, and proteomic analyses reveal that protein arginine methyltransferases post-transcriptionally regulate intron detention—introns that persist in nuclear polyadenylated RNA—through methylation of RNA splicing and processing factors.

Transcriptome and eCLIP analyses in mouse and human reveal splicing factor proline/glutamine rich (SFPQ) as a conserved and critical guardian of long-intron integrity, splicing, and circular RNA (circRNA) production.

A majority of transcribed transposons during aging are derived from transcriptional defects, most notably intron retention and transcriptional readthrough.

Human plasma contains protein-protected mRNA fragments, myriad repeat RNAs, and novel intron RNAs, including a family of structured full-length excised introns, some corresponding to mirtron pre-miRNAs and agotrons.

Group II introns can target mRNA, causing inhibition of gene expression, as a potential driver for evolution to the spliceosome.

Viewing the dynamic interactions of individual spliceosomal subcomplexes with single pre-messenger RNA molecules reveals how nearby flanking splice sites accelerate pre-spliceosome assembly and the splicing of multi-intron pre-mRNAs.

An analysis of the genomic features that distinguish conserved from species-specific circular RNAs reveals that the expansion of the downstream intron by insertion of retrotransposons stabilizes circular RNAs' production across 30+ millions years of evolution.

Hundreds of cell growth and stress response genes are controlled by a rare small RNA component of an ancient splicing machinery, providing a raison d'être for its previously unexplained evolutionary conservation.

Urp1 and Urp2 peptides, expressed in CSF-contacting neurons in the spinal canal, prevent abnormal curvature of the zebrafish spine.