Cas12a CRISPR arrays are sensitive to the GC content of their spacers, but AT-rich separator sequences successfully remove this disruptive effect and enable enhanced multiplexed gene targeting and activation.

The single-cell eQTLGen consortium aims to pinpoint the cellular contexts in which disease-causing genetic variants affect gene expression and its regulation.

Metastases-associated fibroblasts show stage-dependent transcriptional plasticity and their rewiring is regulated by Myc.

Spatially distinct ectodermal Wnt and endodermal Shh signals serve to polarize the skate gill skeleton.

Prokaryotes use polycistronic messages for coordinated translation, whereas eukaryotic cells may achieve tunable protein synthesis by packaging monocistronic mRNAs into functional multiplexes via co-transcriptional interallelic coupling and non-canonical histone-H4 functions.

A retracting gradient of the transcription factor Tailless spatiotemporally patterns the Drosophila tail region by modulating the intrinsic dynamics of a regulatory network involving the timer genes caudal, Dichaete, and odd-paired.

Multiplexed mass spectrometry shows how the autophagy gene Atg16l1 regulates macrophage innate immunity against intracellular bacterial pathogens by modulating basal oxidative stress responses.

Transport of double-stranded RNA from parental circulation to progeny using the transmembrane protein SID-1 can occur through multiple routes during C. elegans development and buffer heritable changes in gene expression.

TAF7L acts as a molecular switch that regulates the development of mesenchymal stem cells.

A high throughput, full lifespan microscopic analysis reveals that fission yeast does not age but has a finite replicative lifespan.