Fam20A, a pseudokinase involved in the formation of tooth enamel, binds ATP in an unexpected orientation.

A pseudokinase in the secretory pathway, Fam20A, activates the real Golgi casein kinase, Fam20C, via a unique mechanism that is lost in human diseases.

Phosphorylation of the pseudokinase Ulk4 by Stk36 promotes primary ciliary tip localization of both proteins to facilitate the phosphorylation and activation of Gli in response to Sonic hedgehog.

The pseudokinase-like Legionella effector SidJ catalyzes the glutamylation of bacterial ubiquitin ligases in a calmodulin-dependent manner.

The pseudokinase Tribbles1 is expressed in human monocytes after mycobacterial challenge and can be manipulated to be host protective against mycobacterial infection, due to control of innate immune cell function, in a zebrafish tuberculosis model.

The clinically approved HER2 inhibitor lapatinib causes HER2 and HER3 kinase domains to dimerise in a non-canonical, symmetric orientation, providing a platform for oligomerisation and predisposing to receptor-driven cell proliferation.

Active site migration establishes kinase activity in protein O-mannose kinase.

Structure-function analysis reveals an allosteric mechanism activating a plant immune receptor kinase complex.

The articles in this special issue highlight how modern cellular, biochemical, biophysical and computational techniques are allowing deeper and more detailed studies of allosteric kinase regulation.