Open-top Bessel beam two-photon light sheet microscopy have the potential for rapid and accurate nondestructive pathology by visualizing 3D histological information.
Gail McConnell, Johanna Trägårdh ... William Bradshaw Amos
A new type of microscope reveals detail of organelles inside every cell in the entirety of an intact embryo or tissue region over 100 cubic millimeters in volume.
In vivo imaging reveals that gradually increased amount of glucose mediates the heterogeneous functional development of individual β-cells by activating its major downstream calcineurin/NFAT signaling pathway.
A novel single-objective light-sheet microscope with unparalleled spatiotemporal resolution enables imaging and optical manipulation of diverse biological specimens and processes.
Light sheet microscopy was used to establish a method for live imaging of cellular events within Arabidopsis flower and for constructing a 3D model of flower at subcellular resolution.
A new approach uses heat-induced antigen retrieval to enable tissue clearing, multiple-round immunostaining, and expansion microscopy for volumetric imaging of centimeter-scale clinical formalin-fixed paraffin-embedded specimens.
Volumetric trans-scale imaging with a subcellular resolution across a wide field-of-view of 1.5 × 1.0 cm2 has been realized, facilitating the analysis of cellular dynamics during embryogenesis and brain function.
The combination of high-speed light sheet microscopy and suitable data analysis facilitates cell-accurate measurements across entire organs and opens the way to systematic, scale-bridging, in vivo studies of organogenesis.
A one-photon three-dimensional light sculpture technique has been developed and demonstrated for high spatio-temporal resolution photostimulation and imaging of dozens of neurons in the intact mouse brain in vivo.
