Max A Horlbeck, Luke A Gilbert ... Jonathan S Weissman
Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.
Crowding and metabolites in a simulated cellular environment alter protein conformations, modulate interactions of functionally related proteins, and lead to significant dynamic heterogeneity.
Minimal changes allow an ancestral, unfolded peptide to adopt a known fold by repetition, illuminating a possible path for the emergence of folded proteins at the origin of life.
Patrick Hillenbrand, Kerstin C Maier ... Ulrich Gerland
Quantitative analysis of time-dependent transcription data elucidates the signal processing within the genetic network that regulates transcriptional cell cycle oscillations in yeast.
Chris A Smith, Andrew D McAinsh, Nigel J Burroughs
The outer domain of the kinetochore can rotate (swivel) around the centromere, and this rather than intra-kinetochore stretching is coupled to anaphase onset.
Nick E Phillips, Cerys S Manning ... Nancy Papalopulu
Stochasticity introduced computationally into a gene expression oscillator creates heterogeneity in the time of differentiation of identical cells and offers robustness to the progenitor state and the outcome of cell division.
Seemingly redundant homologous transcription factors play distinct and cooperative roles in time-dependent combinatorial gene regulation and enable dynamic control of heterogeneity in the gene responses to environmental stresses.
Computational analysis of MNase-seq data reveals that alternatively positioned nucleosomes are prevalent in both yeast and human cells and create significant heterogeneity within cell populations.
The organisation of the Drosophila embryo into segmental units is orchestrated by combinatorial regulatory interactions between spatially patterned and temporally patterned transcription factors.
