To facilitate human developmental biology research, CRISPR-mediated homologous recombination, tightly inducible gene knockdowns (CRISPRi) and overexpression (CRISPRa) have been efficiently applied to human organoids.
Osteoblasts utilize an elegant mechanism by which they obtain and subsequently synthesize the requisite amino acids to support osteoblast differentiation and bone formation.
Maureen C Lamb, Chathuri P Kaluarachchi ... Tina L Tootle
Collectively migrating cells control their stiffness by Fascin-dependent control of Myosin activity, and this migratory cell stiffness regulates Myosin activity and stiffness within the cellular substrate to ultimately promote migration.
Somatic stem and niche cells in the Drosophila ovary develop from common precursors through regulated proliferative expansion, followed by acquisition of position-specific behaviors, rather than through rigid early specification events.
Tania Moreno-Mármol, Mario Ledesma-Terrón ... Paola Bovolenta
Retinal pigment epithelium flattening is an efficient solution adopted by the fast-developing zebrafish to enable folding of the eye primordia, which contrasts with the proliferation-based mechanism used by amniotes.
Fabio Da Silva, Fariba Jian Motamedi ... Andreas Schedl
Lineage tracing and genetic experiments resolve a long-standing controversy by showing that retinoic acid signaling is active in cardiomyocytes, both during development and after myocardial infarction, and protects damaged hearts from apoptosis.
Alessandro Bonfini, Adam J Dobson ... Nicolas Buchon
The nutrient composition of food alters both the size of gut epithelial cells and the ability of the stem cell niche to control tissue turnover, resulting in changes in size at the organ level.
Robert Becker, Silvia Vergarajauregui ... Felix B Engel
Myogenin promotes centrosome attenuation and establishes the nuclear envelope as the dominant microtubule organization center via the scaffold protein AKAP6, which is required for the recruitment of centrosomal proteins.
Baz/Par3 and Sanpdo are required for clustering and activation of Notch receptors to allow intra-lineage private communication following asymmetric division of Drosophila epithelial cells.
Benoit Roch, Vincent Abramowski ... Jean-Pierre de Villartay
A viable separation of function XRCC4 knock-in mutant mouse model recapitulates some aspects of XRCC4 deficiency in humans, notably the absence of immune deficiency.
