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    1. Computational and Systems Biology
    2. Physics of Living Systems

    A coma pattern-based autofocusing method resolves bacterial cold shock response at single-cell level

    Sihong Li, Zhixin Ma ... Shuqiang Huang
    A robust autofocusing method enables continuous single-cell imaging during rapid cooling, revealing sustained bacterial proliferation hidden by growth arrest inferred from optical-density measurements.
    1. Cell Biology
    2. Neuroscience

    Activity-dependent CO2 production in the axon triggers opening of Connexin32 in the Schwann cell paranode

    Jack Butler, Lowell Mott ... Nicholas Dale
    Gaseous CO2 acts as a neuron to glia messenger in peripheral myelin to trigger opening of Schwann Cell Cx32 hemichannels in response to axonal action potential propagation.
    1. Developmental Biology
    2. Genetics and Genomics

    Flexible and high-throughput simultaneous profiling of gene expression and chromatin accessibility in single cells

    Volker Soltys, Moritz Peters ... Yingguang Frank Chan
    Revised
    Reviewed Preprint v3
    Updated
    • Useful
    • Solid
    1. Biochemistry and Chemical Biology

    A simplified and highly efficient cell-free protein synthesis system for prokaryotes

    Xianshengjie Lang, Changbin Zhang ... Wenfei Li
    Revised
    Reviewed Preprint v3
    Updated
    • Valuable
    • Solid
    1. Developmental Biology

    Trpv4 links environmental temperature to testicular differentiation in hermaphroditic ricefield eel

    Yang Zhi, Luo Tingting ... Sun Yuhua
    Revised
    Reviewed Preprint v3
    Updated
    • Useful
    • Incomplete
    1. Neuroscience

    If Grid Cells are the Answer, What is the Question? A Review of Normative Grid Cell Theory

    William Dorrell, James Whittington
    Not revised
    Reviewed Preprint v1
    1. Neuroscience

    Efficient and reproducible pipelines for spike sorting large-scale electrophysiology data

    Alessio P Buccino, Arjun Sridhar ... Joshua H Siegle
    Revised
    Reviewed Preprint v2
    Updated
    • Valuable
    • Compelling
    1. Cell Biology

    CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

    Maria Giovanna De Leo, Andreas Mayer
    Revised
    Reviewed Preprint v2
    Updated
    • Important
    • Compelling
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    ATP-driven conformational dynamics reveal hidden intermediates in a heterodimeric ABC transporter

    Matija Pečak, Christoph Nocker, Robert Tampé
    Revised
    Reviewed Preprint v2
    Updated
    • Important
    • Convincing
    1. Neuroscience

    NPAS4 refines spatial and temporal firing in CA1 pyramidal neurons

    Anja Payne, Daniel A Heinz ... Brenda L Bloodgood
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
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