Rapid and efficient CRISPR/Cas9-mediated knock-in of fluorescent reporters at various genomic loci enables cell- and tissue-specific expression and establishes the short-lived African killifish as a vertebrate system for precise genetic engineering at scale.
Valerie A Tornini, Liyun Miao ... Antonio J Giraldez
Two putative long noncoding RNAs in zebrafish encode micropeptides with homology to the vertebrate-specific chromatin architectural protein, Hmgn1, which are required for development of vertebrate-specific brain cell types.
Well-coordinated, decisive, and collective actions remain critical to make screening programmes more equitable and resilient in the face of natural and geo-political calamities.
Lack of aryl hydrocarbon receptor ligands in the diet of mice increases the inflammatory state in their epidermis, making cutaneous allergic reactions more severe and airway allergy more pronounced after skin sensitization.
The lifespan of individuals who suffer from an osteoporotic fracture is reduced by between 1 and 7 years, depending on the location of the fracture and their risk profile, suggesting that their 'Skeletal Age' is higher than their chronological age.
Shamayeeta Ray, Samuel P Berry ... Rachelle Gaudet
Structures of a transition metal transporter spanning the entire Mn2+ transport cycle reveal distinct coordination geometries and dynamic polar networks that enable Mn2+ import, and a Cd2+-bound structure helps explain how Cd2+ behaves differently as a substrate.
SGLT2 inhibitors reduce podocyte lipotoxicity and improve kidney function in experimental Alport syndrome through a mechanism that involves a switch from the utilization of glucose to fatty acids as an energy substrate in podocytes.
Gut macrophages produce complement component C1q, which modulates neurogenic activity of gut peristalsis and is thus a key regulator of gastrointestinal motility.
