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Page 64 of 93
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    The origins and relatedness structure of mixed infections vary with local prevalence of P. falciparum malaria

    Sha Joe Zhu, Jason A Hendry ... Gil McVean
    Variation in the rate of mixed infections by malaria parasites and the relatedness structure among infecting strains reveals diversity in local epidemiological processes.
    1. Developmental Biology

    In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer

    Li He, Richard Binari ... Norbert Perrimon
    A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology

    Amal Alex, Valentina Piano ... Stefano Maffini
    An electroporation-based method allows the direct delivery of recombinant proteins into mammalian cells for in vivo functional studies.
    1. Cell Biology

    In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation

    Alison K Gillingham, Jessie Bertram ... Sean Munro
    Relocation of Rab, Ras and Rho family GTPases to the surface of mitochondria enables efficient identification of their effectors, exchange factors and GAPs by proximity biotinylation.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Identifying gene expression programs of cell-type identity and cellular activity with single-cell RNA-Seq

    Dylan Kotliar, Adrian Veres ... Pardis C Sabeti
    A novel algorithm, consensus non-negative matrix factorization (cNMF), accurately identifies gene expression programs underlying cell-type identity and cellular activities from single-cell RNA-Seq data.
    1. Ecology

    Genetic transformation of the dinoflagellate chloroplast

    Isabel C Nimmo, Adrian C Barbrook ... Christopher J Howe
    Successful stable transformation of the dinoflagellate chloroplast genome.
    1. Physics of Living Systems

    A microfluidic device for inferring metabolic landscapes in yeast monolayer colonies

    Zoran S Marinkovic, Clément Vulin ... Pascal Hersen
    Metabolic gradients underlie gene expression patterns within spatially controlled expanding 2D microbial colonies.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Genetic interactions of G-quadruplexes in humans

    Katherine G Zyner, Darcie S Mulhearn ... Shankar Balasubramanian
    For the first time, the spectrum of genes and pathways interacting with alternative DNA structures called G-quadruplexes in humans is revealed highlighting new possibilities for the pursuit of cancer therapies.
    1. Structural Biology and Molecular Biophysics

    PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

    Sergey Gorelick, Genevieve Buckley ... Alex de Marco
    The PIE-scope resolves the limitations in correlative cryo-focused ion beam milling by adding robustness and ease of use to the workflow.
    1. Neuroscience

    Imaging neuropeptide release at synapses with a genetically engineered reporter

    Keke Ding, Yifu Han ... David J Anderson
    A series of genetically-encoded fluorescent reporters that allow imaging of neuropeptide release in vivo, with sub-second temporal and nerve terminal-level spatial resolution are developed.