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    1. Chromosomes and Gene Expression

    Exploration of CTCF post-translation modifications uncovers Serine-224 phosphorylation by PLK1 at pericentric regions during the G2/M transition

    Brian C Del Rosario, Andrea J Kriz ... Jeannie T Lee
    The chromosome architectural protein, CTCF, is phosphorylated at Ser224 in a cell-cycle-dependent manner and abrogation of phosphorylation leads to a cell growth defect.
    1. Genetics and Genomics

    Quantitative proteomics reveals key roles for post-transcriptional gene regulation in the molecular pathology of facioscapulohumeral muscular dystrophy

    Sujatha Jagannathan, Yuko Ogata ... Robert K Bradley
    The myopathic transcription factor DUX4 induces discordant dysregulation of transcript and protein levels, demonstrating a key role for post-transcriptional gene regulation in facioscapulohumeral muscular dystrophy.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles

    Maia Azubel, Stephen D Carter ... Roger D Kornberg
    Specific attachment of molecularly defined gold nanoparticles enables precise localization, critical for structural studies in vivo, of proteins of unknown structure within the cellular milieu by cryo-electron tomography.
    1. Neuroscience

    Bayesian analysis of retinotopic maps

    Noah C Benson, Jonathan Winawer
    A novel Bayesian method of modeling retinotopic maps is more accurate than traditional voxel-wise methods and can be used to automatically derive high-quality maps.
    1. Developmental Biology

    Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins

    Maaike Welling, Manuel Alexander Mohr ... Periklis Pantazis
    Primed Track is an image analysis tool that allows for computational correction of drifting specimen to enable accurate lineage tracing of mammalian pre-implantation development even for larger imaging time intervals.
    1. Neuroscience

    High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo

    Guanghan Meng, Yajie Liang ... Na Ji
    Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.
    1. Neuroscience

    Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning

    Shinji Urata, Tadatsune Iida ... Shigeo Okabe
    A method of generating comprehensive maps of cochlear cells was created and enabled researchers to study characteristics of cellular damage in aged and noise-exposed inner ear.
    1. Neuroscience

    CaImAn an open source tool for scalable calcium imaging data analysis

    Andrea Giovannucci, Johannes Friedrich ... Eftychios A Pnevmatikakis
    CaImAn is an open-software package that equips the neuroscience community with a set of turnkey, fast and scalable solutions to pre-processing problems arising in single cell calcium imaging data analysis.
    1. Genetics and Genomics
    2. Stem Cells and Regenerative Medicine

    Global DNA methylation remodeling during direct reprogramming of fibroblasts to neurons

    Chongyuan Luo, Qian Yi Lee ... Joseph R Ecker
    Induced neuronal cells acquire epigenomic signatures specific to matured neurons.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Structured illumination microscopy combined with machine learning enables the high throughput analysis and classification of virus structure

    Romain F Laine, Gemma Goodfellow ... Clemens F Kaminski
    Machine learning in conjunction with super-resolution imaging allows for the first time to quantitatively analyse large and heterogenous virus samples structure at a high throughput and specificity.