728 results found
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Rapid cell-free forward engineering of novel genetic ring oscillators

    Henrike Niederholtmeyer, Zachary Z Sun ... Sebastian J Maerkl
    Rapid forward engineering can be accomplished using cell-free systems, as demonstrated by the implementation and characterization of novel genetic oscillators in a cell-free system and their consequent transfer to cells.
    1. Computational and Systems Biology
    2. Physics of Living Systems

    Light-driven synchronization of optogenetic clocks

    Maria Cristina Cannarsa, Filippo Liguori ... Roberto Di Leonardo
    Introducing the optorepressilator, a synthetic genetic oscillator that can be synchronized, entrained, and detuned by green light to precisely control periodic gene expression in single cells or entire bacterial populations.
    1. Developmental Biology
    2. Genetics and Genomics

    Feedback between a retinoid-related nuclear receptor and the let-7 microRNAs controls the pace and number of molting cycles in C. elegans

    Ruhi Patel, Himani Galagali ... Alison R Frand
    A genetic oscillator composed of NHR-23 and let-7 family of microRNAs links the molting cycle timer and the heterochronic pathway to regulate the pace of molting in C. elegans and ensure that worms molt only four times.
    1. Cell Biology
    2. Genetics and Genomics

    Novel regulators of islet function identified from genetic variation in mouse islet Ca2+ oscillations

    Christopher H Emfinger, Lauren E Clark ... Alan D Attie
    Analysis of islet calcium oscillation responses will provide the field with a resource with which to determine the strain of interest for their specific islet biology questions.
    1. Computational and Systems Biology
    2. Physics of Living Systems

    Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock

    Alexis B Webb, Iván M Lengyel ... Andrew C Oates
    Time-lapse recording and theoretical analysis of individual cells isolated from the zebrafish segmentation clock reveal that they behave as self-sustained, autonomous oscillators with distinctive noisy dynamics.
    1. Computational and Systems Biology
    2. Chromosomes and Gene Expression

    NF-κB oscillations translate into functionally related patterns of gene expression

    Samuel Zambrano, Ilario De Toma ... Alessandra Agresti
    NF-κB oscillations synchronize to external perturbations as a damped oscillator, producing different transcription dynamics.
    1. Cell Biology
    2. Genetics and Genomics

    Real time, in vivo measurement of neuronal and peripheral clocks in Drosophila melanogaster

    Peter S Johnstone, Maite Ogueta ... Deniz Top
    Locally activatable bioluminescence (LABL) is a genetically encoded reporter that allows real time, in vivo measurement of distinct clocks in different cells and tissues in Drosophila.
    1. Developmental Biology
    2. Physics of Living Systems

    From local resynchronization to global pattern recovery in the zebrafish segmentation clock

    Koichiro Uriu, Bo-Kai Liao ... Luis G Morelli
    Theory explains how transport of gene expression vortices by cell advection may cause intermingled defective and normal segments along the body axis during resynchronization experiments in the zebrafish segmentation clock.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Evolving a 24-hr oscillator in budding yeast

    Gregg A Wildenberg, Andrew W Murray
    New complex traits can evolve through the elimination of genes in multiple unrelated pathways, rather than through the creation of new gene functions.

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