Precise hydrogen-bond stabilization of the neck linker docking onto the catalytic motor domain, is crucial for motor activity and intracellular functions of the bi-directional mitotic kinesin-5 Cin8.

The transport of proteins along flagellar microtubules generates the force that enables flagella to propel cells across surfaces.

Single-molecule tracking at scale, analyzing millions of cells and thousands of compounds per day, demonstrates that measuring protein motion provides mechanistic insights relevant to drug discovery.

A method that involves simultaneous tracking of individual mRNAs and their associated ribosomes can be used to determine when and where individual molecules get translated in living cells.

Highly conserved C-terminal domains of the tumor suppressor BRCA2 are not essential for accumulation at damaged DNA but affect conformation and cell survival, implying roles beyond delivering strand-exchange protein RAD51.

Live-cell single-molecule imaging of DNA repair factors can be used to analyze their localization to DNA lesions and dissect the hierarchy of their recruitment.

A novel analytical workflow to quantitatively track motions of thousands of nucleosomes within chromatin fibers by high-speed atomic force microscopy.

MDGAs are highly diffusive molecules that alter both neuroligin-1 and AMPA receptor dynamics and function, thereby significantly delaying the differentiation of excitatory post-synapses.

In vivo single-molecule imaging demonstrates that Smc5/6 chromatin association depends on ATP hydrolysis and dsDNA binding, requires Nse6 and is modulated by Brc1 after DNA damage.

A new analysis algorithm (DISC) enables accurate analysis of data from high-throughput single-molecule paradigms and reveals a non-cooperative binding mechanism of cyclic nucleotide-binding domains from HCN ion channels.