Increased efficiency of targeted genome editing by simple addition of a bipartite-optimized tag at the N- and C-termini of effector proteins (Cas9 and cytosine-to-thymine base editor) is applicable in organismo and cell culture.

Lentiviral protein transduction offers a new approach for administration of custom-designed nucleases, leading to high-efficacy targeted gene editing and reduced off-target activity after virus-directed delivery of programmable nuclease proteins.

A bacterial protein can be programmed with short RNA molecules to stimulate genome editing at specific sites in human cells.

Chemically modifying the 5´ends of donor DNA improves the efficiency of homology-directed repair and suppresses end-joining reactions, which will facilitate precise gene editing.

A CRISPR-based method enables targeted knockouts and genetic screens in human dendritic cells.

The protein p53 negatively impacts the ability of a CRISPR screen to discriminate between essential and non-essential genes, hence, p53 status should be considered in these screens.

A highly efficient platform to engineer designer mutations in human stem cells using RNA-based delivery of prime editing components.

Prime editor activity reporter (PEAR) is a flexible fluorescent reporter that identifies and enriches prime edited cells.

A genome editing resource for discovering, in Drosophila, the organization of cellular structure and function by phosphoinositide signaling.

Adenine and cytosine base editing of highly homologous HBG proximal promoter identifies novel target sites that result in adult to fetal globin switching without causing 4.9 kb large deletions.