Browse our latest Biochemistry and Chemical Biology articles

The pyruvate kinase contained in the apicoplast organelle of malaria parasites makes the nucleotide triphosphates required for several processes including transcription of the organellar genome.

Upon deletion of threonine deaminases, biosynthesis of isoleucine is rescued by two promiscuous reactions, one emerges under aerobic conditions and the other is naturally active under anaerobic conditions.

Mitochondrial pyruvate transport is required for brown fat thermogenesis.

Study of TbAQP2 adaptations and substrate interactions shows how this aquaglyceroporin enables cellular entry of large antimicrobial agents in Trypanosoma brucei.

Mitochondrial fatty acid synthesis is best known for producing lipoic acid, however, longer acyl chain products regulate mitochondrial electron transport chain assembly, oxidative TCA cycle metabolism, and skeletal myoblast differentiation.

Structural and biochemical analysis of the mechanism of paroxetine binding to the serotonin transporter provides a framework for transporter inhibition and design of small-molecule inhibitors.

High-throughput phenotypic screening followed by unbiased target identification reveals a new molecular glue HQ461 that induces CDK12-DDB1 interaction to promote degradation of Cyclin K via the ubiquitin proteasome system.

Simple guidelines and a checklist are provided for performing high-quality equilibrium binding measurements.

When translation stops, cells require intracellular acidification to turn on the conserved heat shock response during stress, and stress-triggered acidification (common in eukaryotes) is adaptive, promoting cell and population fitness.

Hydrogen-deuterium exchange, electron microscopy, and vesicle reconstitution show how binding of the autophagy adaptor NDP52 to the FIP200 subunit of the ULK1 complex triggers membrane binding in autophagy.