Browse our latest Biochemistry and Chemical Biology articles

Alkylation stress modify mRNAs and results in translation arrest, which activates the ribosome-rescue pathway of trans translation in bacteria.

Phosphatidylinositol 3-phosphate interacts with PfHsp70-1 and stabilizes the Plasmodium digestive vacuole under febrile temperatures.

LOV2GIVe allows to activate Gi proteins non-invasively with innocuous blue light based on a design principle unrelated to light-activated GPCRs (metazoan opsins), thereby expanding the range of potential experimental applications.

A set of ER-localized membrane proteins whose loss causes developmental diseases in humans, assemble with Sec61 into a translocon that facilitates the biogenesis of hundreds of different multi-pass membrane proteins.

Rigorous biochemical and structural analyses reveal the precise topology of cohesin's association with DNA and suggest a mechanism for how DNA is transported inside the ring.

Protein engineering of a reversibly switchable fluorescent protein enables post-hoc reversible and repeatable marking of elevated calcium concentrations using blue light.

Methods that use puromycin to localize active translation do not actually detect the site of translation.

Although puromycin staining is often used to examine subcellular translation, puromycin-labeled proteins are rapidly released from ribosomes even in the presence of elongation inhibitors, which may confound translation site localization.

Endothelial Differentiation Factor 1 (EDF1) plays a critical role in driving mRNA-specific quality control and global transcriptional responses in response to ribosome collisions.

In starving yeast exposed to thermal stress, a transient drop in intracellular pH helps to trigger the heat shock response.