Evgeniya Trofimenko, Gianvito Grasso ... Christian Widmann
Cationic cell-penetrating peptides (CPPs) enter cells through ~2 (–5)-nm-wide water pores induced by the strong negative plasma membrane potential that CPPs and the activity of potassium channels generate.
Through interactions with both ubiquitin units that emanate from a branch point in polyubiquitin, UCH37 recognizes and hydrolyzes branched chains to promote proteasome-mediated degradation upon proteolytic stresses.
Domenica Ippolito, Saurabh Thapliyal, Dominique A Glauser
Ca2+-dependent nuclear entry of Ca2+/calmodulin-dependent protein kinase-1 in sensory neurons, taking place with a relatively slow kinetics, contributes to couple long-lasting sensory stimulations with signaling in the nucleus over a timescale relevant for sensory history-dependent plasticity.
Ligand-specific-induced conformations of M3 muscarinic receptors reveal similar requirements for G protein-coupled receptor kinase 2 and Gq protein binding, whereas arrestin3 binding is mediated by distinct receptor conformations.
Maria Podinovskaia, Cristina Prescianotto-Baschong ... Anne Spang
A novel, inexpensive, easy-to-use method to analyse traffic along the endosomal pathway in mammalian cells shows that there is coordination between Rab conversion and acidification, but almost none between Rab conversion and recycling.
SNARE–SNARE interactions that have previously only been proposed to participate in membrane fusion on the nanoscale also serve to organize SNARE domains on the mesoscale.
A proximity labeling approach defines germ granule proteome in Caenorhabditis elegans, and identifies LOTUS domain proteins as key regulators of germ granule assembly.
