CTP and centromere-like parS-DNA act synergistically and catalytically to activate ParB, which remains activated after leaving parS to tune ParA–ParB and ParB–ParB interaction dynamics for robust ParABS-mediated F-plasmid DNA segregation.
Jasmin van den Heuvel, Caroline Ashiono ... Ulrike Kutay
Endoproteolytic cleavage of the ribosomal precursor protein FUBI-eS30 is required for efficient maturation of small ribosomal subunits in the cytoplasm of human cells and involves the nucleolar deubiquitinase USP36.
André Brás Gonçalves, Sarah Kirstine Hasselbalch ... Lotte Bang Pedersen
Analysis of mutant cells combined with biochemical experiments reveals that CEP78 is recruited to the centrosome by CEP350 and regulates ciliogenesis and ciliary length control through CP110-dependent and CP110-independent mechanisms, respectively.
The three-dimensional and asymmetrical transformations undergone by the zebrafish linear heart tube during looping morphogenesis are under the control of cardiac-intrinsic cues.
Ashley L Arthur, Amy Crawford ... Margaret A Titus
Filopodia formation requires recruitment and activation of myosin through generation of parallel, bundled actin filaments by VASP polymerase, revealing coupling between motor activation and organization of a local actin network.
Measurement of physiological intracellular Ca2+ signals following neural stimulation in vivo reveals distinct spatiotemporal characteristics that promote optimal salivary gland fluid secretion.
Galectin-9-deficient mice develop spontaneous autoimmunity, driven by a decreased threshold of B cell activation and enhanced auto-antigen delivery to the spleen by increased B1a-derived autoantibodies.
Saskia MA de Man, Gooitzen Zwanenburg ... Renée van Amerongen
Genome editing, advanced live-cell microscopy, and computational modeling were combined to measure WNT/CTNNB1 signaling parameters at the single molecule level, revealing critical regulatory nodes in this important signal transduction pathway.
Miriam Böhm, Kerstin Killinger ... Stefan Westermann
The cell cycle machinery regulates the assembly of the multi-protein kinetochore complex by targeting excess subunits for degradation via the proteasome.
The balance between SOX2 and SOX21 is evolutionary conserved and is important for cell fate decision during lung epithelium development and repair after damage.
