OptoGranules reveal the function of G3BP1 as a stress granule scaffold and demonstrate that protracted stress granule assembly is sufficient to drive neurodegeneration and the evolution of ALS-FTD pathology.
Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
In epithelial cells, anillin organizes the medial-apical actomyosin cortex into a contractile load-bearing structure and increases tissue-level stiffness.
The SNAP-25 linker acts as a functional component of SNARE complexes, initially facilitating SNARE interactions and later promoting fusion triggering and pore evolution by local membrane contacts.
Kerstin Klinkert, Nicolas Levernier ... Pierre Gönczy
Experiments reveal mechanisms through which Caenorhabditis elegans zygotes depleted of Aurora A or lacking centrosomes spontaneously establish two posterior PAR-2 domains, one at each pole, in a curvature-dependent manner.
The extent of (proteotoxic) endoplasmic reticulum stress, and the ensuing unfolded protein response activation, are commensurate with the extent of the chaperone BiP being sequestered by its client proteins.
Angelika Manhart, Téa Aleksandra Icheva ... Alex Mogilner
Cells are able to control the actin network length and steering by adjusting geometric, structural, or biochemical parameters such as ADF/Cofilin and actin concentrations, and network width.
Rolf M Schmidt, Julia P Schessner ... Sebastian Schuck
Endoplasmic reticulum stress in yeast activates not only the UPR but also Rpn4, promoting the clearance of misfolded proteins from the cytosol as part of a modular cross-compartment stress response.
