The protein Pat1 functions in the assembly of processing bodies, cellular membraneless organelles, by promoting the liquid–liquid phase separation of the DEAD-box ATPase Dhh1 and RNA.
By distributing regulation to both the GTPase and downstream kinases, the Mitotic Exit Network creates a single signal from spatial and temporal inputs.
Fabian E Ortega, Elena F Koslover, Julie A Theriot
The pathogenic bacterium Listeria monocytogenes spreads infection using a two-tiered strategy, where most bacteria spread locally but a few 'pioneers' move further, increasing the likelihood of a persistent infection.
Nicholas R Ader, Patrick C Hoffmann ... Wanda Kukulski
Correlative microscopy and electron cryo-tomography on apoptotic HeLa cells reveal remodeling of outer and inner mitochondrial membranes, Bax cluster ultrastructure and ATP synthase reorganization in ruptured mitochondria.
In Drosophila oocytes, the exclusion of the scaffold protein PAR3 from the posterior cortex depends on PAR1 and endocytosis, while its anterior localisation requires microtubules and recycling endosomes.
Martina Pannuzzo, Zachary A McDargh, Markus Deserno
Computational modeling of a dynamin-constricted membrane neck can rank proposed shape changes of the helical scaffold in terms of their fission aptitude and elucidate the associated pathways.
Eric A Prinslow, Karolina P Stepien ... Josep Rizo
Biophysical analyses indicate that Munc18-1, Munc13-1, synaptotagmin-1 and complexin-1 maintain assembled trans-SNARE complexes in the presence of NSF-alphaSNAP, suggesting that they form part of the primed state of synaptic vesicles.
