A sophisticated classical mutagenesis assay with next-generation sequencing technology presents a benefit to future research on mutagenesis, DNA repair, and cancer.
Christian Keine, Mohammed Al-Yaari ... Samuel M Young Jr
A combination of genetic, electrophysiological, and modeling approaches reveals that presynaptic Rac1 is a key molecule that controls synaptic strength and plasticity by regulating the synaptic vesicle cycle steps controlling the number of fusion competent synaptic vesicles.
Publicly available gene expression data and proven methodologies for building model Gene Regulatory Networks are applied to successfully construct the IGF1 signaling GRN behind alveologenesis, serving as a pioneer in transforming how lung development and its associated diseases is approached.
Inactivation of SURF4 leads to impaired secretion of PCSK9 and apolipoproteins resulting in low plasma cholesterol without detrimental consequences to the liver.
Endothelial sphingolipid biosynthesis is essential for maintaining vascular development, neovascular proliferation, non-CNS tissue sphingolipid levels, and hepatocyte response to stress.
Ricardo A Cordova, Jagannath Misra ... Kirk A Staschke
The ISR kinase GCN2 is critical for maintaining tumor amino acid levels to facilitate growth, suggesting a novel therapeutic strategy for the treatment of prostate cancer by inducing starvation for essential amino acids.
Dirk H Siepe, Lukas T Henneberg ... K Christopher Garcia
A proteo-genomic CRISPR activation screening workflow to accelerate the identification of novel interactions between the secreted and membrane proteome with the potential to define new biological processes and identify future therapeutic targets.
Nuno GB Neto, Sinead A O'Rourke ... Michael G Monaghan
Human blood derived macrophage polarisation can be classified by proxy of their metabolism, using advanced microscopy techniques generating single-cell parameters that are clustered and validated using machine learning classification models.
An improved culture method for explanted adult Drosophila organs allows the live-imaging of damage response, cell differentiation, and tracing of progenitor cell lineages through multiple rounds of division.
