A deep learning-based image classification pipeline unleashes automated division counting and replicative lifespan analyses of single cells growing in microfluidic cellular traps.
Bronwyn A Lucas, Kexin Zhang ... Nikolaus Grigorieff
Comparing the relative similarity of cellular molecules to alternate templates allows classification of related complexes in the cell using fewer particles than needed for 3D classification and is more practicable for low abundance complexes.
Local ATP production by the plasma membrane-associated glycolytic enzyme pyruvate kinase is essential for the nutrient-dependent closure of the ATP-sensitive potassium channels that initiate insulin release from pancreatic β-cells.
Brain-specific inactivation of cohesin-STAG2 in the mouse causes myelination defects, thus implicating hypomyelination as a contributing factor to cohesinopathy and establishing oligodendrocytes as a cell system to probe the physiological function of cohesin-mediated genome folding.
Helena Schulz-Mirbach, Alexandra Müller ... Steffen N Lindner
The amination network for the distribution of assimilated ammonium throughout metabolism is defined by promiscuous transaminases, allowing a high flexibility of the network that supported engineering novel connections and even alternative ammonium assimilation entry points.
Lise-Marie Donnio, Elena Cerutti ... Giuseppina Giglia-Mari
After DNA damage induction, transcription is blocked and XAB2 is released from R-loops allowing a subset of XAB2 molecules to interact with UV-stalled RNAP2 and participating to the TC-NER process.
Alice L Herneisen, Zhu-Hong Li ... Sebastian Lourido
Time-resolved phosphoproteomics and thermal proteome profiling reveal the Ca2+-responsive proteome of the model apicomplexan Taxoplasma gondii, identifying PP1 as a Ca2+-responsive enzyme that regulates Ca2+ uptake to promote parasite motility.
