The c-terminus of PI3K plays a key role in regulating kinase activity, with c-terminal disease-linked mutations leading to either activation or inhibition, which reveal a path to specific inhibitors.
Spatial restriction of GPCR signaling at nascent synapses by adhesion complexes drives excitatory synapse formation and specificity in the hippocampus.
A comparative analysis of human and chimpanzee polyadenylation site usage establishes alternative polyadenylation as another key mechanism underlying the genetic regulation of transcript and protein expression levels in primates.
Raymond F Pauszek III, Rajan Lamichhane ... David P Millar
The primer extension and 5' flap processing activities of DNA polymerase I are physically coordinated by combined movements of the DNA substrate and the 5' nuclease domain of the enzyme.
Taylor B Updegrove, Jailynn Harke ... Kumaran S Ramamurthi
Reengineering the nucleotide-binding pocket of an extant ATPase to restore ancestral GTPase activity revealed an ATP-dependent intermediate required for function and suggested why the protein evolved to use ATP.
Phase separation of two-component multivalent systems is suppressed at rational polymer stoichiometries, suggesting possible cellular strategies for regulating the formation and function of biomolecular condensates.
