16,523 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Conformational distributions of isolated myosin motor domains encode their mechanochemical properties

    Justin R Porter, Artur Meller ... Gregory R Bowman
    Shifts in the balance between nucleotide-favorable and nucleotide-unfavorable conformations of myosin motors encode duty ratios and ADP release rates, demonstrating the power of an ensemble perspective for uncovering sequence-function relationships.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading

    Hayley Porter, Yang Li ... Suzana Hadjur
    STAG proteins function independently of core cohesin at R-loops to facilitate cohesin loading.
    1. Immunology and Inflammation

    Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice

    Laura Medina-Ruiz, Robin Bartolini ... Gerard J Graham
    The use of multi-chemokine receptor reporter mice helps to unravel the dynamics of receptor involvement in leukocyte migration in vivo and suggests specificity, rather than redundancy, in receptor use.
    1. Computational and Systems Biology
    2. Epidemiology and Global Health

    Bias in nutrition-health associations is not eliminated by excluding extreme reporters in empirical or simulation studies

    Nao Yamamoto, Keisuke Ejima ... Andrew W Brown
    Elimination of extreme reporters using Goldberg cutoffs does not always produce unbiased estimates of associations between nutrition intakes and health outcomes.
    1. Chromosomes and Gene Expression

    Reporter-ChIP-nexus reveals strong contribution of the Drosophila initiator sequence to RNA polymerase pausing

    Wanqing Shao, Sergio G-M Alcantara, Julia Zeitlinger
    Reporter-ChIP-nexus captures paused Pol II on transfected plasmids and shows that pausing stability is influenced by core promoter sequences, especially by the initiator with a G at +2 position.
    1. Plant Biology

    Building customizable auto-luminescent luciferase-based reporters in plants

    Arjun Khakhar, Colby G Starker ... Daniel F Voytas
    A fungal bioluminescence pathway can be reconstituted in planta to create luminescence in many plant species without external substrate addition, and be used to design customizable reporters of gene-expression.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

    Dorottya Anna Simon, András Tálas ... Ervin Welker
    Prime editor activity reporter (PEAR) is a flexible fluorescent reporter that identifies and enriches prime edited cells.
    1. Cancer Biology
    2. Cell Biology

    One reporter for in-cell activity profiling of majority of protein kinase oncogenes

    Iva Gudernova, Silvie Foldynova-Trantirkova ... Pavel Krejci
    A new luciferase and fluorescent reporter system enables rapid and efficient in-cell profiling of the majority of protein kinase oncogenes known to date.
    1. Chromosomes and Gene Expression

    Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers

    Timothy W R Kelso, Devin K Porter ... Diana C Hargreaves
    ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, and its homolog ARID1B function to maintain chromatin accessibility and active histone modifications at enhancers.
    1. Immunology and Inflammation
    2. Neuroscience

    MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis

    Em P Harrington, Riley B Catenacci ... Peter A Calabresi
    Oligodendroglia express major histocompatibility complex (MHC) pathways in response to inflammation and MHC reporter mice allow for the investigation of MHC molecule expressing cells in vivo.

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