A CRISPR/Cas9 base editor screen allows probing interactions between large numbers of known mutations and the abundance of specific proteins to study the protein regulatory network in yeast.

Aerobic glycolysis in rod photoreceptors of adult mice is regulated by allostery and FGF signaling and is required for maintaining outer segments.

Metabolic switches between oxidative phosphorylation and aerobic glycolysis plus glutaminolysis direct T cell function by altering the flux of glucose and glutamine to N-glycosylation.

A new pooled screening method in yeast allows scientists to probe how protein levels are regulated by mutating thousands of genes at once.

In mice, but not in humans, the bone-derived hormone osteocalcin is O-glycosylated, a post-translational modification controlling its half-life in vivo.

A primary constraint for mitochondrial repression is access to cytosolic inorganic phosphate, which is determined by the glycolytic flux rate.

Synechocystis switches its redox pools under reducing photomixotrophic conditions from utilizing NAD(H)- to ferredoxin-dependent enzymes and thereby balances its metabolism in a trade-off between energy conservation and chemical driving forces.

Achieving autotrophy in E. coli by introducing a small number of genetic changes crosses a milestone in engineering major metabolic transitions and paves a way for sustainable bio-production from CO₂.