A highly efficient platform to engineer designer mutations in human stem cells using RNA-based delivery of prime editing components.

Strategic pegRNA designs and other approaches mitigating 5' and 3' interactions within pegRNAs can often lead to substantial improvements in prime editing efficiency.

Prime editor activity reporter (PEAR) is a flexible fluorescent reporter that identifies and enriches prime edited cells.

In P3 editing, molecular interactions are sensed and converted directly into genome editing events, which may be useful for biological recording and synthetic molecular circuits deployed in living cells.

Increased efficiency of targeted genome editing by simple addition of a bipartite-optimized tag at the N- and C-termini of effector proteins (Cas9 and cytosine-to-thymine base editor) is applicable in organismo and cell culture.

Closely related type II-C Cas9 orthologs recognize diverse PAMs, which facilitates us to expand the targeting scope.

Molecular signals from chloroplasts can synergistically interact with the plant hormone, abscisic acid (ABA), to regulate non-canonical signaling pathways mediating fundamental cellular processes including stomatal closure, seed dormancy and germination.

Brain recovery after injury can be predicted based on its activity and structure, which may allow us to understand why some brain injuries lead to permanent loss of cognitive function, while others do not.

Metabolic biomarkers measured from single blood test can identify apparently healthy people at high susceptibility for developing severe pneumonia, and may also be useful for preventive COVID-19 screening.

The Pro-PRIME model demonstrates the ability to engineer VHH antibody with unprecedented alkali resistance and enhanced affinity, enabling industrial applications under extreme conditions previously unattainable with natural proteins.