Clonal heterozygosity for recessive disease alleles can elicit unexpected phenotypes in vivo, suggesting a new genetic concept relevant to understanding pathogenesis (deleterious heteromosaicism).
Madhav Jagannathan, Ryan Cummings, Yukiko M Yamashita
Satellite DNA mediates clustering of multiple chromosomes via formation of chromocenter to encapsulate the full complement of the genome into a single nucleus.
Structures known as chromocenters, comprising satellite DNA and proteins such as D1 or HMGA1, help to contain DNA inside the nucleus between cell divisions.
Pooran Singh Dewari, Benjamin Southgate ... Steven M Pollard
Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.
Proteasomes are protected from autophagic elimination upon carbon starvation by sequestration into cytoplasmic storage granules, which aid cell fitness by providing a cache of proteasomes that can be rapidly remobilized when carbon availability improves.
Plant-unique RAB5 effector 2 (PUF2) is an effector of plant-unique ARA6, which plays a key role in plant endosomal transport, integrating functions of the two plant RAB5 groups by an unprecedented mechanism.
A single-cell assay reveals that the genetic rewiring that underlies PARP inhibitor resistance drives altered DNA double-strand break end resection pathway choice.
Sharan Janjuha, Sumeet Pal Singh ... Nikolay Ninov
During aging, the pancreatic endocrine islets undergo chronic inflammation, which is associated with a reduced capacity for self-renewal of the insulin-producing beta-cells in zebrafish.
