Endothelial sphingolipid biosynthesis is essential for maintaining vascular development, neovascular proliferation, non-CNS tissue sphingolipid levels, and hepatocyte response to stress.
Ricardo A Cordova, Jagannath Misra ... Kirk A Staschke
The ISR kinase GCN2 is critical for maintaining tumor amino acid levels to facilitate growth, suggesting a novel therapeutic strategy for the treatment of prostate cancer by inducing starvation for essential amino acids.
Dirk H Siepe, Lukas T Henneberg ... K Christopher Garcia
A proteo-genomic CRISPR activation screening workflow to accelerate the identification of novel interactions between the secreted and membrane proteome with the potential to define new biological processes and identify future therapeutic targets.
Nuno GB Neto, Sinead A O'Rourke ... Michael G Monaghan
Human blood derived macrophage polarisation can be classified by proxy of their metabolism, using advanced microscopy techniques generating single-cell parameters that are clustered and validated using machine learning classification models.
An improved culture method for explanted adult Drosophila organs allows the live-imaging of damage response, cell differentiation, and tracing of progenitor cell lineages through multiple rounds of division.
Audrey Miller Williams, Seth Donoughe ... Sally Horne-Badovinac
Fat2 signals from the trailing edge of each cell to localize WAVE activity to the leading edge of the cell behind, polarizing protrusive regions within cells and across the epithelium.
Edmundo G Vides, Ayan Adhikari ... Suzanne R Pfeffer
Parkinson’s disease-associated LRRK2 kinase is recruited to membranes by its Rab GTPase substrates, and LRRK2 is both retained on membranes and further activated there by cooperative interaction with the phosphorylated Rab proteins that it generates.
