The archaeal MCM helicase can load in multiple orientations on DNA but translocation proceeds with a leading N-terminal domain, which affects double hexamer activation at origins of replication.
Cassandra L Stowe, Thomas A Burley ... Martin A Pule
Two cellular populations can be tracked in the same small animal model using near-infrared bioluminescence imaging, for the first time opening up the window for multi-coloured bioluminescence.
David Massa López, Melanie Thelen ... Markus Damme
The so far uncharacterized lysosomal transporter protein MFSD1 is essential for liver homeostasis and needs the highly glycosylated GLMP protein as an accessory subunit for stability.
Julia R Lazzari-Dean, Anneliese MM Gest, Evan W Miller
Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.
Hemoglobin affinity and cooperativity reveal mechanistic insights in how the relation between physiology and evolutionary variations shape a protein's molecular property.
Xiaocheng Zhao, Pavel Nedvetsky ... Holger Gerhardt
The cAMP-dependent protein kinase A controls the switch from actively sprouting new blood vessel formation to vessel quiescence by reducing endothelial autophagy through phosphorylation-mediated destabilisation of ATG16L1.
Shannon N Mostyn, Katie A Wilson ... Robert J Vandenberg
Identification of how bioactive lipids bind and inhibit glycine transporters has the potential to be exploited in the development of a new class of analgesics for chronic pain.
The signaling ligand (p)ppGpp regulates the enzyme HPRT across species by binding to a novel class of conserved motif, yet its specificity is allosterically altered through evolution of enzyme oligomerization.
