Conditional gene knockout in combination with fluorescent labeling of different alleles can be achieved with high efficiency in zebrafish through NHEJ-mediated targeted insertion using a CRISPR/Cas system.
Luis F Sifuentes-Dominguez, Haiying Li ... Ezra Burstein
A novel mutation in SCGN is associated with risk for inflammatory bowel disease and implicates the intestinal neuroendocrine compartment in the pathogenesis of this disorder.
Herpes simplex ICP4 preferentially binds to and delineates the viral genome, ultimately resulting in robust viral transcription at the expense of the host.
Daniel P Murphy, Andrew EO Hughes ... Joseph C Corbo
While photoreceptor and bipolar cells exhibit very similar cis-regulatory grammars, subtle differences in homeodomain motif enrichment represent a key distinction driving the divergence in their transcriptomes.
A genetic screen in a unicellular photosynthetic organism uncovers the first essential signaling component in the chloroplast unfolded protein response that relays information from the chloroplast to the nuclear compartment.