Curated by
Roger Davis et al.

Reproducibility Project: Cancer Biology

Investigating reproducibility in preclinical cancer research.
Collection

The Reproducibility Project: Cancer Biology is an initiative to independently replicate selected results from a number of high-profile papers in the field of cancer biology. For each paper a Registered Report detailing the proposed experimental designs and protocols for the replications is peer reviewed and published prior to data collection; the results of these experiments are then published as a Replication Study. The project is a collaboration between the Center for Open Science and Science Exchange.

The aim of the project is two-fold: to provide evidence about reproducibility in preclinical cancer research, and to identify the factors that influence reproducibility more generally. Interpreting the results reported in the Replication Studies requires a nuanced approach, as explained in this Editorial. To date four of the studies have reproduced important parts of the original papers, one study has not, and another two studies could not be interpreted.

Collection

    1. Biochemistry
    2. Cancer Biology

    Replication Study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

    Megan Reed Showalter et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    Replication Study
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology

    Replication Study: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia

    Xiaochuan Shan et al
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    Replication Study
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology

    Replication Study: BET bromodomain inhibition as a therapeutic strategy to target c-Myc

    Fraser Aird et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    Replication Study
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology

    Replication Study: Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs

    Christine Mantis et al.
    Editors' Summary: This Replication Study did not reproduce those experiments in the original paper that it attempted to reproduce.
    Replication Study
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology

    Replication Study: Discovery and preclinical validation of drug indications using compendia of public gene expression data

    Irawati Kandela et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    Replication Study
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology

    Replication Study: The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors

    Stephen K Horrigan, Reproducibility Project: Cancer Biology
    Editors' Summary: The results in this Replication Study could not be interpreted.
    Replication Study
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology

    Replication Study: Melanoma genome sequencing reveals frequent PREX2 mutations

    Stephen K Horrigan et al.
    Editors' Summary: The results in this Replication Study could not be interpreted.
    Replication Study
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genes and Chromosomes

    Registered report: Systematic identification of genomic markers of drug sensitivity in cancer cells

    John P Vanden Heuvel et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine

    Registered report: Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs

    Mitch Phelps et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine

    Registered report: Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF

    Ajay Bhargava et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine

    Registered report: Diverse somatic mutation patterns and pathway alterations in human cancers

    Vidhu Sharma et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    Registered report: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation

    Vidhu Sharma et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine

    Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation

    Adam D Richarson et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine

    Registered report: Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma

    John Repass et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine

    Registered report: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth

    Ajay Bhargava et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    Registered report: A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations

    Babette Haven et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Genes and Chromosomes

    Registered report: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology

    Israr Khan et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Human Biology and Medicine

    Registered report: Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases

    Brad Evans et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine

    Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET

    Jake Lesnik et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    Registered report: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment

    James Evans et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization

    Francesca Incardona et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells
    2. Human Biology and Medicine

    Registered report: Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells

    Denise Chroscinski et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine
    2. Microbiology and Infectious Disease

    Registered report: Intestinal inflammation targets cancer-inducing activity of the microbiota

    Kate Eaton et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Human Biology and Medicine

    Registered report: Senescence surveillance of pre-malignant hepatocytes limits liver cancer development

    Samrrah Raouf et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    Registered report: Transcriptional amplification in tumor cells with elevated c-Myc

    David Blum et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    Registered report: Widespread potential for growth factor-driven resistance to anticancer kinase inhibitors

    Edward Greenfield et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion

    David Blum et al
    Registered Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf