Reproducibility Project
Edited by
Roger J Davis et al.

Reproducibility Project: Cancer Biology

Investigating reproducibility in preclinical cancer research.
Collection
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The Reproducibility Project: Cancer Biology is an initiative to independently replicate selected results from a number of high-profile papers in the field of cancer biology. For each paper a Registered Report detailing the proposed experimental designs and protocols for the replications is peer reviewed and published prior to data collection; the results of these experiments are then published as a Replication Study. The project is a collaboration between the Center for Open Science and Science Exchange.

The aim of the project is two-fold: to provide evidence about reproducibility in preclinical cancer research, and to identify the factors that influence reproducibility more generally. Interpreting the results reported in the Replication Studies requires a nuanced approach, as explained in this Editorial. To date five of the studies have reproduced important parts of the original papers; six of the studies have reproduced parts of the original papers but also contain results that could not be interpreted or are not consistent with some parts of the original paper; two of the studies could not be interpreted; and three studies did not reproduce the parts of the original papers that they attempted to reproduce.

Collection

    1. Cancer Biology

    Replication Study: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology

    John Kerwin et al.
    Editors' Summary: This Replication Study has reproduced some parts of the original paper but it also contains results that are not consistent with other parts of the original paper.
    1. Cancer Biology

    Replication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis

    Mee Rie Sheen et al.
    Editors' Summary: This Replication Study has reproduced some parts of the original paper but other parts could not be interpreted.
    1. Cancer Biology

    Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment

    Anthony Essex et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44

    Xuefei Yan et al.
    Editors' Summary: This Replication Study did not reproduce those experiments in the original paper that it attempted to reproduce.
    1. Cancer Biology

    Replication Study: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET

    Jeewon Kim et al.
    Editors' Summary: This Replication Study has reproduced some parts of the original paper but other parts could not be interpreted.
    1. Cancer Biology

    Replication Study: Intestinal inflammation targets cancer-inducing activity of the microbiota

    Kathryn Eaton et al.
    Editors' Summary: This Replication Study has reproduced some parts of the original paper but it also contains results that are not consistent with other parts of the original paper.
    1. Cancer Biology

    Replication Study: Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma

    John Repass, Reproducibility Project: Cancer Biology
    Editors' Summary: This Replication Study did not reproduce those experiments in the original paper that it attempted to reproduce.
    1. Cancer Biology

    Replication Study: Transcriptional amplification in tumor cells with elevated c-Myc

    L Michelle Lewis et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper, but it also contains results that are not consistent with some parts of the original paper.
    1. Cancer Biology

    Replication Study: Systematic identification of genomic markers of drug sensitivity in cancer cells

    John P Vanden Heuvel et al.
    Editors' Summary: This Replication Study has reproduced some parts of the original paper but other parts could not be interpreted.
    1. Cancer Biology

    Replication Study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

    Megan Reed Showalter et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia

    Xiaochuan Shan et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: BET bromodomain inhibition as a therapeutic strategy to target c-Myc

    Fraser Aird et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs

    Christine Mantis et al.
    Editors' Summary: This Replication Study did not reproduce those experiments in the original paper that it attempted to reproduce.
    1. Cancer Biology

    Replication Study: Discovery and preclinical validation of drug indications using compendia of public gene expression data

    Irawati Kandela et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors

    Stephen K Horrigan, Reproducibility Project: Cancer Biology
    Editors' Summary: The results in this Replication Study could not be interpreted.
    1. Cancer Biology

    Replication Study: Melanoma genome sequencing reveals frequent PREX2 mutations

    Stephen K Horrigan et al.
    Editors' Summary: The results in this Replication Study could not be interpreted.
    1. Cancer Biology

    Registered report: Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs

    Mitch Phelps et al.
    1. Cancer Biology

    Registered report: Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF

    Ajay Bhargava et al.
    1. Cancer Biology

    Registered report: Diverse somatic mutation patterns and pathway alterations in human cancers

    Vidhu Sharma et al.
    1. Cancer Biology

    Registered report: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation

    Vidhu Sharma et al.
    1. Cancer Biology

    Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation

    Adam D Richarson et al.
    1. Cancer Biology

    Registered report: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth

    Ajay Bhargava et al.
    1. Cancer Biology

    Registered report: A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations

    Babette Haven et al.
    1. Cancer Biology

    Registered report: Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases

    Brad Evans et al.
    1. Cancer Biology

    Registered report: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment

    James Evans et al.
    1. Cancer Biology

    Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization

    Francesca Incardona et al.
    1. Cancer Biology

    Registered report: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis

    Steven Fiering et al.
    1. Cancer Biology

    Registered report: Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells

    Denise Chroscinski et al.
    1. Cancer Biology

    Registered report: Senescence surveillance of pre-malignant hepatocytes limits liver cancer development

    Samrrah Raouf et al.
    1. Cancer Biology

    Registered report: Widespread potential for growth factor-driven resistance to anticancer kinase inhibitors

    Edward Greenfield et al.
    1. Cancer Biology

    Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion

    David Blum et al.

Related

    1. Biochemistry and Chemical Biology
    2. Neuroscience

    Science Forum: Addressing selective reporting of experiments through predefined exclusion criteria

    Kleber Neves, Olavo B Amaral
    1. Cancer Biology

    Reproducibility in Cancer Biology: Pseudogenes, RNAs and new reproducibility norms

    George A Calin
    1. Cancer Biology

    Reproducibility in Cancer Biology: Rethinking research into metastasis

    Peter Friedl
    1. Cancer Biology

    Reproducibility in Cancer Biology: The who, where and how of fusobacteria and colon cancer

    Cynthia L Sears
    1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Reproducibility in Cancer Biology: Getting to grips with c-Myc

    Dirk Eick
    1. Cancer Biology

    Reproducibility in Cancer Biology: The challenges of replication

    1. Cancer Biology

    Reproducibility in Cancer Biology: Making sense of replications

    Brian A Nosek, Timothy M Errington
    1. Cancer Biology

    Reproducibility in Cancer Biology: Small molecules remain on target for c-Myc

    Linchong Sun, Ping Gao
    1. Cancer Biology

    Reproducibility in Cancer Biology: Mixed outcomes for computational predictions

    Chi Van Dang
    1. Cancer Biology

    Reproducibility in Cancer Biology: Melanoma mystery

    Roger J Davis
    1. Cancer Biology

    Reproducibility Project: Cancer Biology: Time to do something about reproducibility

    Sean J Morrison
    1. Human Biology and Medicine

    Science Forum: An open investigation of the reproducibility of cancer biology research

    Timothy M Errington et al.

Contributors

  1. Roger J Davis
    Reviewing Editor
  2. Joaquin Espinosa
    Reviewing Editor
  3. Michael R Green
    Reviewing Editor
  4. Joan Massagué
    Reviewing Editor
  5. Duojia Pan
    Reviewing Editor