Curated by
Roger Davis et al.

Reproducibility Project: Cancer Biology

Investigating reproducibility in preclinical cancer research.
Collection

The Reproducibility Project: Cancer Biology is an initiative to independently replicate selected results from a number of high-profile papers in the field of cancer biology. For each paper a Registered Report detailing the proposed experimental designs and protocols for the replications is peer reviewed and published prior to data collection; the results of these experiments are then published as a Replication Study. The project is a collaboration between the Center for Open Science and Science Exchange.

The aim of the project is two-fold: to provide evidence about reproducibility in preclinical cancer research, and to identify the factors that influence reproducibility more generally. Interpreting the results reported in the Replication Studies requires a nuanced approach, as explained in this Editorial. To date four of the studies have reproduced important parts of the original papers, one study has not, and another two studies could not be interpreted.

Collection

    1. Biochemistry
    2. Cancer Biology

    Replication Study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

    Megan Reed Showalter et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia

    Xiaochuan Shan et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: BET bromodomain inhibition as a therapeutic strategy to target c-Myc

    Fraser Aird et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs

    Christine Mantis et al.
    Editors' Summary: This Replication Study did not reproduce those experiments in the original paper that it attempted to reproduce.
    1. Cancer Biology

    Replication Study: Discovery and preclinical validation of drug indications using compendia of public gene expression data

    Irawati Kandela et al.
    Editors' Summary: This Replication Study has reproduced important parts of the original paper.
    1. Cancer Biology

    Replication Study: The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors

    Stephen K Horrigan, Reproducibility Project: Cancer Biology
    Editors' Summary: The results in this Replication Study could not be interpreted.
    1. Cancer Biology

    Replication Study: Melanoma genome sequencing reveals frequent PREX2 mutations

    Stephen K Horrigan et al.
    Editors' Summary: The results in this Replication Study could not be interpreted.