Chemically modifying the 5´ends of donor DNA improves the efficiency of homology-directed repair and suppresses end-joining reactions, which will facilitate precise gene editing.

Increased efficiency of targeted genome editing by simple addition of a bipartite-optimized tag at the N- and C-termini of effector proteins (Cas9 and cytosine-to-thymine base editor) is applicable in organismo and cell culture.

A bacterial protein can be programmed with short RNA molecules to stimulate genome editing at specific sites in human cells.

Optimised genome editing in P. knowlesi enables transgenic expression of a lead P. vivax vaccine candidate, revealing roles in host cell tropisms and providing tools for scalable vaccine efficacy testing.

Antibody paratopes can be added to the human repertoire by CRISPR-Cas9 editing of B lymphocytes using a strategy that allows for hypermutation and class switching of resulting B cell receptors.

CRISPR genome editing technology can efficiently introduce mutations into lytic and latent HSV genomes to block lytic replication and reactivation of latent herpes simplex virus genome though differential mechanisms.

Novel Cas9 fusion with DNA polymerase delta subunit 3 (POLD3), identified via high-throughput screening of more than 450 DNA repair proteins, enhances editing by speeding up the initiation of DNA repair.

Genome editing in the choanoflagellate Salpingoeca rosetta opens newfound possibilities to functionally probe choanoflagellate genes that may illuminate the origin of their closest relatives, the animals.

An EASAC working group on genome editing recommends that regulators should focus on specific applications of these new techniques rather than attempting to regulate genome editing itself as a new technology.

CRISPR/Cas9 based genomic editing for efficient endogenous protein tagging can be achieved by targeting non-coding sequences with two guides.