A novel genome-wide transcriptional activation screening in Drosophila cells revealed the activation of InR-Akt-mTOR pathway by cholesterol in plasma membrane to confer resistance to Rapamycin.

Both exogenous and endogenous genes can be highly activated in cells by a new dCas9-based activator, CRISPR-assisted trans enhancer, that can be used to activate genes for biomedical applications.

Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.

A novel strategy to create genetic mosaics in zebrafish for cell type-specific analysis of gene function that can be applied to any gene of interest.

A novel tool is used to assess gene function in vitro by targeted endogenous transcriptional activation to unveil novel players in development and disease.

CRISPR genome editing technology can efficiently introduce mutations into lytic and latent HSV genomes to block lytic replication and reactivation of latent herpes simplex virus genome though differential mechanisms.

Med16 and GSK3b regulate interferon gamma-mediated MHCII expression in macrophages and are required for CD4+ T cell activation.

Cas12a CRISPR arrays are sensitive to the GC content of their spacers, but AT-rich separator sequences successfully remove this disruptive effect and enable enhanced multiplexed gene targeting and activation.

A proteo-genomic CRISPR activation screening workflow to accelerate the identification of novel interactions between the secreted and membrane proteome with the potential to define new biological processes and identify future therapeutic targets.

Kiss1^ARH neurons transition from synchronous to burst firing under preovulatory levels of E2, causing a shift from peptidergic to glutamatergic transmission that drives the GnRH surge through enhanced glutamate neurotransmission.