Genetic and biochemical analyses reveal that two stem-cell-specific microRNAs control stem cell fate decisions between pluripotency and differentiation through repressing Ago2, a key component of the microRNA machinery.
Pamela Himadewi, Xue Qing David Wang ... Xiaotian Zhang
The 3'HS1 CTCF binding site directly modulates chromosomal loops to regulate γ-globin expression through the accession of the hereditary persistence of fetal hemoglobin enhancer.
Cas12a CRISPR arrays are sensitive to the GC content of their spacers, but AT-rich separator sequences successfully remove this disruptive effect and enable enhanced multiplexed gene targeting and activation.
Reconstitution of orientation-specific R-loop-replisome collisions with purified proteins reveals the differential impact of R-loop-associated RNA:DNA hybrids and G-quadruplexes on replication fork progression.
YAP and TAZ, the ultimate targets of the Hippo signaling pathway, are transcriptional co-activators of AP-1 proteins and STAT3 through which they are important for breast cellular transformation.
Although the preinitiation complex for transcriptional initiation by RNA polymerase II contains highly conserved general transcription factors, there are kinetic and compositional differences in the initiation process among eukaryotic species.
Luka Bacic, Guillaume Gaullier ... Sebastian Deindl
Analysis of conformational heterogeneity in cryo-EM data by a recently developed deep learning method reveals the structure and dynamics of the oncogenic chromatin remodeler ALC1 bound to a poly-ADP-ribosylated nucleosome.
David M Garcia, Edgar A Campbell ... Daniel F Jarosz
In its capacity to switch into a heritable, prionogenic form, a conserved RNA-modifying enzyme epigenetically alters fundamental growth, aging, and protein synthesis properties of eukaryotic cells.
Michele Felletti, Cédric Romilly ... Kristina Jonas
Specific amino acids in the N-terminus of the replication initiator protein DnaA inhibit translation elongation upon carbon starvation, illustrating that the identity of the N-terminal amino acids of a protein can modulate protein synthesis yield under changing conditions.
Alessandro Stirpe, Nora Guidotti ... Thomas Schalch
Clr4, a highly conserved SUV39 histone methyltransferase, requires its SET domain to sense histone H3K14 ubiquitination in order to maintain heterochromatin and H3K9 methylation.
