Reproducibility Project Cancer Biology

The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange to independently replicate selected results from a substantial number of high-profile papers in the field of cancer biology. For each paper a Registered Report detailing the proposed experimental designs and protocols for the replications is peer reviewed and published prior to data collection. The results of these experiments will be published in a Replication Study. The project will provide evidence about reproducibility in cancer biology, and an opportunity to identify factors that influence reproducibility more generally.

Articles

  1. Biochemistry

COT drives resistance to RAF inhibition through MAP kinase pathway reactivation

Original Article

  1. CM Johannessen
  2. JS Boehm
  3. SY Kim
  4. SR Thomas
  5. L Wardwell
  6. LA Johnson
  7. CM Emery
  8. N Stransky
  9. AP Cogdill
  10. J Barretina
  11. et al.
Nature 2010;468:968-972

Replication Study

  1. Human Biology and Medicine

IDH mutation impairs histone demethylation and results in a block to cell differentiation

Registered Report

  1. Adam D Richarson
  2. David A Scott
  3. Olga Zagnitko
  4. Pedro Aza-Blanc
  5. Chih-Cheng Chang
  6. David A Russler-Germain
  7. Reproducibility Project: Cancer Biology
eLife 2016;5:e10860

Replication Study

  1. Biochemistry
  2. Cell Biology

The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

Replication Study

  1. Biochemistry

A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations

Original Article

  1. Sreenath V Sharma
  2. Diana Lee
  3. Bihua Li
  4. Margaret P Quinlan
  5. Fumiyuki Takahashi
  6. Shyamala Maheswaran
  7. Ultan McDermott
  8. Nancy Azizian
  9. Lee Zou
  10. Michael A Fischbach
  11. et al.
Cell 2010;141:69-80

Registered Report

  1. Babette Haven
  2. Elysia Heilig
  3. Cristine Donham
  4. Michael Settles
  5. Nicole Vasilevsky
  6. Katherine Owen
  7. Reproducibility Project: Cancer Biology
eLife 2016;5:e09462

Replication Study

  1. Human Biology and Medicine

Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF

Replication Study

  1. Human Biology and Medicine

RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth

Original Article

  1. G Hatzivassiliou
  2. K Song
  3. I Yen
  4. BJ Brandhuber
  5. DJ Anderson
  6. R Alvarado
  7. MJ Ludlam
  8. D Stokoe
  9. SL Gloor
  10. G Vigers
  11. et al.
Nature 2010;464:431-435

Registered Report

  1. Ajay Bhargava
  2. Steven Pelech
  3. Ben Woodard
  4. John Kerwin
  5. Nimet Maherali
  6. Reproducibility Project: Cancer Biology
eLife 2016;5:e09976

Replication Study

  1. Human Biology and Medicine

Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma

Original Article

  1. M Castellarin
  2. RL Warren
  3. JD Freeman
  4. L Dreolini
  5. M Krzywinski
  6. J Strauss
  7. R Barnes
  8. P Watson
  9. E Allen-Vercoe
  10. RA Moore
  11. et al.
Genome Research 2012;22:299-306

Replication Study

  1. Human Biology and Medicine

Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET

Original Article

  1. H Peinado
  2. M Alečković
  3. S Lavotshkin
  4. I Matei
  5. B Costa-Silva
  6. G Moreno-Bueno
  7. M Hergueta-Redondo
  8. C Williams
  9. G García-Santos
  10. CM Ghajar
  11. et al.
Nature Medicine 2012;18:883-891

Replication Study

  1. Human Biology and Medicine

Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukemia

Registered Report

  1. Juan José Fung
  2. Alan Kosaka
  3. Xiaochuan Shan
  4. Gwenn Danet-Desnoyers
  5. Michael Gormally
  6. Kate Owen
  7. Reproducibility Project: Cancer Biology
eLife 2015;4:e08997

Replication Study

  1. Developmental Biology and Stem Cells

Wnt activity defines colon cancer stem cells and is regulated by the microenvironment

Original Article

  1. L Vermeulen
  2. EMF De Sousa
  3. M van der Heijden
  4. K Cameron
  5. JH de Jong
  6. T Borovski
  7. JB Tuynman
  8. M Todaro
  9. C Merz
  10. H Rodermond
  11. et al.
Nature Cell Biology 2010;12:468-476

Registered Report

  1. James Evans
  2. Anthony Essex
  3. Hong Xin
  4. Nurith Amitai
  5. Lindsey Brinton
  6. Erin Griner
  7. Reproducibility Project: Cancer Biology
eLife 2015;4:e07301

Replication Study

  1. Biophysics and Structural Biology
  2. Cell Biology

Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis

Registered Report

  1. Steven Fiering
  2. Lay-Hong Ang
  3. Judith Lacoste
  4. Tim D Smith
  5. Erin Griner
  6. Reproducibility Project: Cancer Biology
eLife 2015;4:e04796

Replication Study

  1. Developmental Biology and Stem Cells

Interactions between cancer stem cells and their niche govern metastatic colonization

Registered Report

  1. Francesca Incardona
  2. M Mehdi Doroudchi
  3. Nawfal Ismail
  4. Alberto Carreno
  5. Erin Griner
  6. Minyoung Anna Lim
  7. Reproducibility Project: Cancer Biology
eLife 2015;4:e06938

Replication Study

  1. Human Biology and Medicine
  2. Microbiology and Infectious Disease

Intestinal inflammation targets cancer-inducing activity of the microbiota

Original Article

  1. JC Arthur
  2. E Perez-Chanona
  3. M Muhlbauer
  4. S Tomkovich
  5. JM Uronis
  6. TJ Fan
  7. BJ Campbell
  8. T Abujamel
  9. B Dogan
  10. AB Rogers
  11. et al.
Science 2012;338:120-123

Replication Study

  1. Developmental Biology and Stem Cells
  2. Human Biology and Medicine

Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells

Original Article

  1. L Ricci-Vitiani
  2. R Pallini
  3. M Biffoni
  4. M Todaro
  5. G Invernici
  6. T Cenci
  7. G Maira
  8. EA Parati
  9. G Stassi
  10. LM Larocca
  11. et al.
Nature 2010;468:824-828

Replication Study

  1. Human Biology and Medicine

The CD47-signal regulated protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors

Original Article

  1. SB Willingham
  2. JP Volkmer
  3. AJ Gentles
  4. D Sahoo
  5. P Dalerba
  6. SS Mitra
  7. J Wang
  8. H Contreras-Trujillo
  9. R Martin
  10. JD Cohen
  11. et al.
Proceedings of the National Academy of Sciences of USA 2012;109:6662-6667

Replication Study

  1. Biochemistry

Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion

Original Article

  1. R Straussman
  2. T Morikawa
  3. K Shee
  4. M Barzily-Rokni
  5. ZR Qian
  6. J Du
  7. A Davis
  8. MM Mongare
  9. J Gould
  10. DT Frederick
  11. et al.
Nature 2012;487:500-504

Replication Study

  1. Biochemistry

Widespread potential for growth factor-driven resistance to anticancer kinase inhibitors

Replication Study