February 2014

Research articles

    1. Biophysics and Structural Biology
    2. Immunology

    Structural basis for the prion-like MAVS filaments in antiviral innate immunity

    Hui Xu et al.
    Mitochondrial anti-viral signaling proteins form three-stranded helical filaments in launching innate immune responses against RNA viruses.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Neuroscience

    The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites

    Jana Dorfman Marcette et al.
    The minus-ends of microtubules have been implicated as part of a damage sensor to initiate remodeling of nerve cells.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Neuroscience

    Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment

    Huisheng Liu et al.
    Two proteins—synaptotagmin 7 and calmodulin—have central roles in the calcium-dependent pathway that maintains synaptic transmission.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector

    Matteo Allegretti et al.
    The structure of a large enzyme complex involved in methanogenesis was determined at high resolution by electron cryo-microscopy.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    The structure and organization of lanceolate mechanosensory complexes at mouse hair follicles

    Lishi Li, David D Ginty
    Close examination of lanceolate mechanosensory complexes has revealed clues about the ways that sensory nerves detect the movement of hairs and shown than terminal Schwann cells are needed to maintain and regenerate these intricate structures.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy

    Koji Yamano et al.
    Two GAP proteins bound to mitochondria regulate the enyzme Rab7, and thereby the expansion of the isolation membrane during mitophagy, downstream of PINK1 and Parkin, two proteins that are mutated in familial Parkinson's disease.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Neuroscience

    PTRN-1, a microtubule minus end-binding CAMSAP homolog, promotes microtubule function in Caenorhabditis elegans neurons

    Claire E Richardson et al.
    The protein PTRN-1 anchors microtubules in neuronal branches to promote proper neuron morphology and function in Caenorhabditis elegans.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genes and Chromosomes
    2. Genomics and Evolutionary Biology

    The life cycle of Drosophila orphan genes

    Nicola Palmieri et al.
    The short lifetime of orphan genes is a major factor explaining the stability of gene number in Drosophila.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis

    Satdip Kaur et al.
    Endocytosis is switched off in dividing cells because actin is not available to assist the clathrin machinery at a time when membrane tension is increased.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Immunology
    2. Microbiology and Infectious Disease

    Identification of an LGP2-associated MDA5 agonist in picornavirus-infected cells

    Safia Deddouche et al.
    A viral RNA has been identified as a specific MDA5 agonist in cells infected with encephalomyocarditis virus.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Human Biology and Medicine

    miR-146a promotes the initiation and progression of melanoma by activating Notch signaling

    Matteo Forloni et al.
    The microRNA, miR-146a, is regulated by mutant BRAF/NRAS proteins, and promotes the development of melanoma via increasing Notch signaling pathway, suggesting that combining drugs targeting BRAF/NRAS and Notch may provide an effective melanoma treatment.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Auditory synapses to song premotor neurons are gated off during vocalization in zebra finches

    Kosuke Hamaguchi et al.
    Intracellular recordings in singing birds and images of synapses in deafened birds provide insights into the neural circuitry that enables songbirds to fine-tune their songs.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Single molecule FRET reveals pore size and opening mechanism of a mechano-sensitive ion channel

    Yong Wang et al.
    Observation by single molecule FRET of MscL, a prokaryotic mechanosensitive channel, reveals that MscL opens via the helix-tilt model and its pore reaches 2.8 nm in diameter.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells
    2. Genes and Chromosomes

    Essential role of lncRNA binding for WDR5 maintenance of active chromatin and embryonic stem cell pluripotency

    Yul W Yang et al.
    RNA binding is a key input of an active chromatin complex and maintenance of stem cell fate.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Cell Biology

    Small molecule-mediated refolding and activation of myosin motor function

    Michael B Radke et al.
    The small molecule EMD 57033 is one of a new class of pharmacological chaperones that stabilize, enhance the activity of, and correct stress-induced misfolding of myosin proteins.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Developmental Biology and Stem Cells

    Plasticity of both planar cell polarity and cell identity during the development of Drosophila

    Pedro Saavedra et al.
    In the Drosophila larval epidermis both the cell identity and polarity of individual cells change as a result of cell rearrangements.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Microbiology and Infectious Disease

    Escherichia coli swimming is robust against variations in flagellar number

    Patrick J Mears et al.
    The swimming behavior of E. coli is surprisingly robust against the natural variations in the number of flagella per cell.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Cell Biology

    Diversity and plasticity in Rab GTPase nucleotide release mechanism has consequences for Rab activation and inactivation

    Lars Langemeyer et al.
    Analysis of multiple guanine exchange factors shows that Rab activation can occur via a number of mechanistically distinct GDP-release pathways.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Genomics and Evolutionary Biology

    Endogenous RNA interference is driven by copy number

    Cristina Cruz, Jonathan Houseley
    The ability to target transcripts from high-copy regions of the genome is an emergent property of a minimal RNA interference system.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Plant Biology

    Automated quantitative histology reveals vascular morphodynamics during Arabidopsis hypocotyl secondary growth

    Martial Sankar et al.
    Combining high-resolution imaging with automated image segmentation and supervised machine learning achieves accurate cellular feature extraction and automated cell type recognition in a large-scale developmental process.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Munc18-1 is a dynamically regulated PKC target during short-term enhancement of transmitter release

    Özgür Genç et al.
    Protein kinase C brings about post-tetanic potentiation - a temporary increase in synaptic strength due to increased transmitter release - via phosphorylation of a target protein, Munc18-1.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology
    2. Microbiology and Infectious Disease

    Integrating influenza antigenic dynamics with molecular evolution

    Trevor Bedford et al.
    Combined antigenic and genetic analysis shows that different strains of the human influenza virus display dramatically different rates of antigenic drift, and that these differences have a significant impact on the number of new infections in each flu season.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Biophysics and Structural Biology

    Super Spy variants implicate flexibility in chaperone action

    Shu Quan et al.
    Lab-evolved 'super Spy' chaperones show enhanced flexibility, which allows them to bind to and stabilize proteins more effectively than natural chaperones.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Identification of Redeye, a new sleep-regulating protein whose expression is modulated by sleep amount

    Mi Shi et al.
    A gene found in Drosophila, and named redeye, encodes a protein that accumulates during sleep deprivation and forms part of the homeostatic system that promotes and maintains sleep.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Genes and Chromosomes

    Shugoshin biases chromosomes for biorientation through condensin recruitment to the pericentromere

    Kitty F Verzijlbergen et al.
    Shugoshin proteins help to align chromosomes so that copies of the same chromosome attach to microtubules from opposite poles during cell division.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Cell Biology

    Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting

    Florian Wilfling et al.
    The targeting of proteins from the endoplasmic reticulum to lipid droplets relies on surface modulation of the lipid droplet by the Arf1/COPI machinery.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    An allosteric Sec61 inhibitor traps nascent transmembrane helices at the lateral gate

    Andrew L MacKinnon et al.
    A small molecule, cotransin, blocks transmembrane domains form integrating into cell membranes by allosterically ‘locking’ the lateral gate of the Sec61 translocation channel.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf