January 2015

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Research articles

    1. Genomics and Evolutionary Biology
    2. Microbiology and Infectious Disease

    Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses

    Michael B Schulte et al
    A mathematical model that combines stochasticity and spatial structure describes the dynamics of the viral population during an infection cycle, and fitting the model to RNA and virus abundances over time shows that poliovirus follows a geometric replication mode.
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    1. Biochemistry
    2. Microbiology and Infectious Disease

    Angiomotin functions in HIV-1 assembly and budding

    Gaelle Mercenne et al
    Angiomotin (AMOT) is a cellular host factor that links HIV-1 to the ubiquitin E3 ligase, NEDD4L, and promotes virion assembly and envelopment.
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    1. Microbiology and Infectious Disease

    Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses

    Ci-Xiu Li et al
    Extensive genetic diversity and novel genome structures in RNA viruses from arthropods shed important new light on the ancestry and evolutionary history of major classes of vertebrate and plant viruses.
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    1. Genes and Chromosomes
    2. Genomics and Evolutionary Biology

    A recurrent regulatory change underlying altered expression and Wnt response of the stickleback armor plates gene EDA

    Natasha M O'Brown et al
    An identical DNA base pair change has occurred in divergent sticklebacks to alter the expression of a major developmental gene controlling armor plates, thus providing a molecular link between particular enhancer changes, cell signaling, and repeated skeletal evolution in natural populations.
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    1. Neuroscience

    Learning the specific quality of taste reinforcement in larval Drosophila

    Michael Schleyer et al
    The finding that fly maggots, equipped with only 10,000 neurons, process reinforcement not only by value but also by specific quality reveals a basic operating principle of brains and challenges current models of memory organization.
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    1. Neuroscience

    A cortical disinhibitory circuit for enhancing adult plasticity

    Yu Fu et al
    Building on previous work (Kaneko and Stryker, 2014), we report that the specific population of inhibitory neurons that increases the responses of neurons in the visual cortex during locomotion will also, when activated or blocked artificially, dramatically alter adult cortical plasticity, revealing that the aerobic exercise of locomotion is not necessary.
    Research Advance
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    1. Genomics and Evolutionary Biology

    Enhanced stability and polyadenylation of select mRNAs support rapid thermogenesis in the brown fat of a hibernator

    Katharine R Grabek et al
    In the torpid hibernator, when transcription is inhibited, enhanced stability and polyadenylation explain increased abundance of crucial transcripts required for intense non-shivering thermogenesis during arousal.
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    1. Biophysics and Structural Biology
    2. Cell Biology

    Non-catalytic motor domains enable processive movement and functional diversification of the kinesin-14 Kar3

    Christine Mieck et al
    The kinesin-14 motor Kar3 moves along microtubules using a previously undescribed mechanism that critically requires the presence of a non-catalytic head.
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    1. Genomics and Evolutionary Biology

    Genes associated with ant social behavior show distinct transcriptional and evolutionary patterns

    Alexander S Mikheyev, Timothy A Linksvayer
    Genes associated with age-based division of labor in ants exist in distinct regulatory and evolutionary contexts; genes up-regulated in young nurses are loosely connected and rapidly evolving while genes up-regulated in old foragers are highly connected and conserved.
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    1. Biochemistry
    2. Genomics and Evolutionary Biology

    Translation of 5′ leaders is pervasive in genes resistant to eIF2 repression

    Dmitry E Andreev et al
    Most of the mRNAs whose translation is resistant to the stress-induced repression of protein synthesis contain upstream open reading frames that are efficiently translated under normal conditions.
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    1. Cell Biology
    2. Human Biology and Medicine

    Defective apical extrusion signaling contributes to aggressive tumor hallmarks

    Yapeng Gu et al
    Disrupting extrusion, a process that drives epithelial cell death, leads to increased cell survival, poor barrier function, and enhanced cell invasion and, thereby, promotes tumor initiation and progression.
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    1. Neuroscience

    Temporal structure in associative retrieval

    Zeb Kurth-Nelson et al
    Neural representations are fast-evolving trajectories, and distinct components of these trajectories reappear during retrieval with distinct consequences for learning.
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    1. Biochemistry
    2. Cell Biology

    A molecular basis for the differential roles of Bub1 and BubR1 in the spindle assembly checkpoint

    Katharina Overlack et al
    The mechanism behind the recruitment of the spindle assembly checkpoint protein BubR1 to the kinetochore illustrates how gene duplication and sub-functionalization can influence the functional complexity of a protein network.
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    1. Immunology
    2. Microbiology and Infectious Disease

    Phenotypic complementation of genetic immunodeficiency by chronic herpesvirus infection

    Donna A MacDuff et al
    HOIL-1 deficiency in mice results in a severe immunodeficiency that is effectively complemented by chronic infection with a murine gamma-herpesvirus.
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    1. Cell Biology

    Controlling fertilization and cAMP signaling in sperm by optogenetics

    Vera Jansen et al
    Optogenetics is a non-invasive way to study cAMP signaling in sperm cells, which is able to control sperm motility and fertilization.
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    1. Biophysics and Structural Biology
    2. Genomics and Evolutionary Biology

    Methylation at the C-2 position of hopanoids increases rigidity in native bacterial membranes

    Chia-Hung Wu et al
    Methylation specifically enhances the ability of hopanoids to rigidify membranes under physiologically relevant conditions, which impacts the current interpretation of the 2-methylhopane fossil record.
    Research Article
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    1. Genomics and Evolutionary Biology
    2. Microbiology and Infectious Disease

    Global biogeographic sampling of bacterial secondary metabolism

    Zachary Charlop-Powers et al
    Pyro-sequencing of two genes involved in bacterial secondary metabolism provides new insights into the rich reservoir of biologically active small molecules found in environmental bacteria.
    Short Report
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    1. Biochemistry
    2. Genes and Chromosomes

    Bridged filaments of histone-like nucleoid structuring protein pause RNA polymerase and aid termination in bacteria

    Matthew V Kotlajich et al
    Bridged H-NS filaments inhibit transcript elongation by bacterial RNA polymerase by enhancing backtracking and increasing Rho-dependent termination at a subset of pause sites that are normally poor Rho substrates.
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    1. Genomics and Evolutionary Biology
    2. Microbiology and Infectious Disease

    The contrasting phylodynamics of human influenza B viruses

    Dhanasekaran Vijaykrishna et al
    The analysis of the genomes of two lineages of influenza B virus (Victoria and Yamagata) reveal that their phylodynamics are fundamentally different, and are determined by a complex relationship between virus transmission, age of infection and receptor binding preference.
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    1. Cell Biology

    Reliable cell cycle commitment in budding yeast is ensured by signal integration

    Xili Liu et al
    The decision to commit to cell division-the Start transition-in budding yeast is governed by time integration of G1 cyclin-CDK activity by the transcription factor Whi5.
    Research Article
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    1. Neuroscience

    The topography of frequency and time representation in primate auditory cortices

    Simon Baumann et al
    fMRI data from macaques suggest that sounds with similar temporal characteristics activate neighbouring regions of auditory cortex, giving rise to a topographic map broadly analogous to that for sound frequencies.
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    1. Cell Biology
    2. Developmental Biology and Stem Cells

    Mitochondrial Ca2+ uptake by the voltage-dependent anion channel 2 regulates cardiac rhythmicity

    Hirohito Shimizu et al
    Enhancing mitochondrial Ca2+ uptake effectively suppresses aberrant Ca2+ induced arrhythmogenic events in zebrafish, mouse and human cardiomyocytes, demonstrating a critical role for mitochondria in the regulation of cardiac rhythmicity.
    Research Article
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    1. Neuroscience

    Delay-dependent contributions of medial temporal lobe regions to episodic memory retrieval

    Maureen Ritchey et al
    The anterior and posterior parts of the hippocampus have distinct roles in the retrieval of contextual information in memories of recent experiences.
    Research Article
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    1. Biophysics and Structural Biology
    2. Plant Biology

    Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography

    Benjamin D Engel et al
    The first 3D views of the native algal chloroplast provide new insights into thylakoid biogenesis, photosynthesis, and carbon fixation.
    Research Article
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    1. Neuroscience

    Knockdown of hypothalamic RFRP3 prevents chronic stress-induced infertility and embryo resorption

    Anna C Geraghty et al
    A chronic stressor prior to mating has lasting negative consequences on the fertility and fecundity of female rats, which can be prevented by blocking a single hypothalamic reproductive inhibitory hormone.
    Short Report
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    1. Genes and Chromosomes

    Heritable capture of heterochromatin dynamics in Saccharomyces cerevisiae

    Anne E Dodson, Jasper Rine
    Single-cell and single-molecule analyses of transient gene expression revealed hitherto unrecognized dynamics in heterochromatin function and defined novel roles for a heterochromatin establishment factor and an understudied sirtuin.
    Research Article
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    1. Human Biology and Medicine

    Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs

    Lauran Madden et al
    A novel bioengineered human skeletal muscle model with accurate physiological and pharmacological responses may provide a useful tool for preclinical testing.
    Research Article
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    1. Biophysics and Structural Biology

    Structure of a low-population intermediate state in the release of an enzyme product

    Alfonso De Simone et al
    Product release from human lysozyme is mediated by an intermediate state with transient weak interactions between the product and enzyme.
    Short Report
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    1. Cell Biology
    2. Neuroscience

    Activity and Ca2+ regulate the mobility of TRPV1 channels in the plasma membrane of sensory neurons

    Eric N Senning, Sharona E Gordon
    Channel activity is a novel regulatory mechanism for channel mobility in the plasma membrane.
    Research Article
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    1. Cell Biology
    2. Neuroscience

    Neuron-wide RNA transport combines with netrin-mediated local translation to spatially regulate the synaptic proteome

    Sangmok Kim, Kelsey C Martin
    The spatial regulation of gene expression within neurons occurs primarily at the level of local translation rather than by stimulus-induced RNA targeting from nucleus to synapse.
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    1. Genomics and Evolutionary Biology
    2. Neuroscience

    The majority of transcripts in the squid nervous system are extensively recoded by A-to-I RNA editing

    Shahar Alon et al
    The first example of an animal where RNA-editing dramatically reshapes the entire proteome demonstrates that editing must be a critical evolutionary and adaptive force.
    Short Report
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    1. Immunology

    The transforming growth factor beta signaling pathway is critical for the formation of CD4 T follicular helper cells and isotype-switched antibody responses in the lung mucosa

    Heather D Marshall et al
    Differentiation of CD4 T cells into CD4 T follicular helper cells during viral infection in mice is promoted by tumor growth factor beta.
    Research Article
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    1. Genomics and Evolutionary Biology
    2. Human Biology and Medicine

    A feedback loop between nonsense-mediated decay and the retrogene DUX4 in facioscapulohumeral muscular dystrophy

    Qing Feng et al
    Expression of the disease gene DUX4 inhibits RNA quality control in skeletal muscle, thereby stabilizing thousands of aberrant RNAs, including its own transcript.
    Short Report
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    1. Neuroscience

    A single pair of neurons links sleep to memory consolidation in Drosophila melanogaster

    Paula R Haynes et al
    Dorsal paired medial neurons, which are required for memory consolidation, promote sleep by inhibiting the excitation of mushroom body neurons.
    Research Article
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    1. Cell Biology

    Potassium dependent rescue of a myopathy with core-like structures in mouse

    M Gartz Hanson et al
    A mouse model of human muscle myopathy is used to provide mechanistic insight, identify possible biomarkers of disease, and suggest possible therapeutic strategies to alleviate muscle weakness.
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    1. Biochemistry
    2. Cell Biology

    Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity

    Hermann Broder Schmidt, Dirk Görlich
    How nuclear pore complexes establish their permeability barrier has been a long-standing question; now, this process can be reconstituted by a surprisingly simple and rapid self-assembly of Nup98 FG domains into selective FG phases.
    Research Article
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    1. Neuroscience

    Normalisation of brain connectivity through compensatory behaviour, despite congenital hand absence

    Avital Hahamy et al
    Building on previous work (Makin et al., 2013), we show that the brains of individuals born without a hand adaptively change to compensate for their disability.
    Research Advance
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    1. Developmental Biology and Stem Cells
    2. Genomics and Evolutionary Biology

    Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita

    Karl R Wotton et al
    Quantitative system-level analysis of a pattern-forming gene regulatory network in a non-model organism shows that dynamic changes in gene expression evolve through quantitative system drift.
    Research Article
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    1. Cell Biology
    2. Developmental Biology and Stem Cells

    miR-128 regulates neuronal migration, outgrowth and intrinsic excitability via the intellectual disability gene Phf6

    Eleonora Franzoni et al
    The regulation of Phf6 by miR-128 is a developmental timing mechanism that influences cortical lamination, neuronal morphology and intrinsic excitability.
    Research Article
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    1. Neuroscience

    Interplay between population firing stability and single neuron dynamics in hippocampal networks

    Edden Slomowitz et al
    Neuronal networks balance flexibility with stability by allowing the firing rate of individual neurons within a network to vary over time, while ensuring that the average firing rate across the network remains constant.
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    1. Biophysics and Structural Biology

    Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5′ splice site recognition

    Yasushi Kondo et al
    The complete architecture of human spliceosomal U1 snRNP and its interaction with the 5′ splice site of pre-mRNA are described in atomic detail.
    Research Article
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    1. Biochemistry
    2. Cell Biology

    Proteomic analysis of the response to cell cycle arrests in human myeloid leukemia cells

    Tony Ly et al
    Building on previous work (Ly et al., 2014), we show that elutriated cells and arrested cells have similar patterns of DNA content and cyclin expression: however, a large fraction of the proteome changes detected in arrested cells are found to reflect arrest-specific responses rather than physiological cell cycle regulation.
    Research Advance
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