William M Roberts, Steven B Augustine ... Shawn R Lockery
A stochastic model of locomotory control in C. elegans based on an extensive new set of tracking data can explain and predict effects of ablations and mutations on behavior.
Hamdah Shafqat-Abbasi, Jacob M Kowalewski ... Staffan Strömblad
“Discontinuous” and “Continuous” migration modes are divergent mesenchymal migration strategies that arise spontaneously in parallel in an equilibrium modulated by cell-matrix attachment and actomyosin contractility.
Maarten Hilbrant, Thorsten Horn ... Kristen A Panfilio
Late morphogenesis of the insect extraembryonic epithelia involves transient formation of a basal-basal bilayer to coordinate the functional contributions of two discrete tissues.
Curtis McMurtrey, Thomas Trolle ... William Hildebrand
T. gondii infection alters how peptide ligands are presented to the immune system by inducing a previously unreported structural change in Human Leukocyte Antigens (HLAs).
By properly accounting for gene copy number and cell-cycle effects, single cell snapshots of nascent and mature mRNA can be used to unveil the stochastic kinetics of gene activity.
Assaf Elazar, Jonathan Weinstein ... Sarel Jacob Fleishman
Deep sequencing analysis quantifies the hydrophobicity of the plasma membrane and provides restraints for structure modeling of receptor homodimers to atomic precision.
Martin Steger, Francesca Tonelli ... Matthias Mann
Discovery of a physiological LRRK2 substrate and a new mechanism of Rab regulation should aid Parkinson’s research and the understanding of Rab function.
Marina Theodosiou, Moritz Widmaier ... Reinhard Fässler
Kindlin-2 co-operates with talin to activate fibronectin-binding integrins on fibroblasts and subsequently induces cell spreading by recruiting paxillin to small, peripheral nascent adhesions.
Kawssar Harb, Elia Magrinelli ... Christian Alfano
Lmo4 specifies two neuron subclasses in the mouse neocortex by promoting postnatal co-expression of the transcription factors Ctip2 and Satb2 via chromatin remodelling in a time and area-specific manner.
Joaquim Grego-Bessa, Joshua Bloomekatz ... Kathryn V Anderson
In addition to its role in regulating proliferation and cell death, the PTEN tumor suppressor regulates epithelial morphogenesis through the PDK1 kinase.
Distinct binding of viral proteins to the same region on the nucleosome surface can result in contrasting changes to higher-order chromatin structure in the host cell.
PDGFRα+ Sca-1+ bone marrow stromal/stem cells in whole bone marrow grafts can trigger the onset of autoimmune-related fibrosis in a mouse model of scleroderma.
The protozoan parasite Trypanosoma brucei gambiense has undergone recent clonal evolution that reveals the theoretically predicted Meselson effect at a genome-wide level.
Chloé Habermacher, Adeline Martz ... Thomas Grutter
The gating mechanism of trimeric ATP-gated P2X receptors has been explored using photo-switchable cross-linkers, via a versatile strategy that could be applied to other membrane proteins.
Ellen N Elliott, Karyn L Sheaffer, Klaus H Kaestner
The maintenance methyltransferase Dnmt1 and the de novo methyltransferase Dnmt3b cooperate to maintain DNA methylation and genomic stability in the adult intestinal epithelium.
Peer-Hendrik Kuhn, Alessio Vittorio Colombo ... Stefan F Lichtenthaler
The metalloprotease ADAM10 modulates axon and synapse function by cleaving numerous synaptic and axonal membrane proteins in the central nervous system.
Chandra M Khantwal, Sherwin J Abraham ... Merritt Maduke
The characterization of a previously unidentified “outward-facing open” conformational state provides a new framework for understanding the CLC transport mechanism.
Kirsten I Bos, Alexander Herbig ... Hendrik N Poinar
The analysis of 18th century Y. pestis genomes reveals a bacterial lineage that might be responsible for the 400-year period of European plague epidemics from the Renaissance through early modern times.
Michael N Economo, Nathan G Clack ... Jayaram Chandrashekar
High-resolution fluorescence imaging of the complete mouse brain enables many neurons to be efficiently visualized in their entirety, revealing all targets of neurons that project widely across the brain.
Bart-Jan de Kreuk, Alexandre R Gingras ... Mark H Ginsberg
The proteins Heart of Glass (HEG1) and Rasip1 are both essential for cardiovascular development; and directly bind to each other to guide Rasip1 to endothelial cell junctions to support vascular integrity.
Nikolai Hentze, Laura Le Breton ... Matthias P Mayer
Heat-induced local unfolding allows Hsf1 to form trimers and bind to DNA, which depends on Hsf1 concentration and is promoted, not inhibited, by Hsp90.
The tail domain of Myosin III binds to and cross-links actin bundling protein Espin1 and thus modulates higher order actin bundle structures in cellular processes such as stereocilia and microvilli.
Cerebellar Purkinje cells represent movements via bidirectional linear changes in spike rate, and activity from single cells is sufficient to reconstruct kinematic changes during bouts of free whisking.
Innate lymphoid cells, which are dynamic under steady-state conditions, respond to a colitogenic stimulus by mobilizing from cryptopatches and secreting GM-CSF to organize the pro-inflammatory response.
Caspar M Schwiedrzik, Benjamin Bernstein, Lucia Melloni
Looking at visual motion affects perception of nonsymbolic numerosity in a direction-specific way, indicating that motion and number are computed by the same neurons.
Eva Lana-Elola, Sheona Watson-Scales ... Victor LJ Tybulewicz
A panel of seven new mouse strains with chromosomal duplications is used to identify a minimal genetic region required in three copies to cause congenital heart defects typical of human Down syndrome.
A systems level reconstitution of H-Ras GTPase signaling reveals a rich space of tunable dynamic outputs and clarifies the consequences of oncogenic perturbations to signaling.
Hif/Hif-3α has two distinct roles in cells: activating HRE-dependent gene expression and binding to β-catenin to destabilize the nuclear β-catenin complex.
A reanalysis of the stochastic model of organelle production (Mukherji and O'Shea, 2014) suggests that this model requires significant further discussion.
Kang Wang, Pedro Mateos-Aparicio ... John P Adelman
The slow afterhyperpolarization that follows a burst of action potentials is a powerful regulator of neuronal excitability and is not due to IK1 (KCNN4), a member of the SK channel family.
Zachary B Katz, Brian P English ... Robert H Singer
A method that involves simultaneous tracking of individual mRNAs and their associated ribosomes can be used to determine when and where individual molecules get translated in living cells.
Christina M Zimanyi, Percival Yang-Ting Chen ... Catherine L Drennan
Substrate and effector bound structures of a bacterial ribonucleotide reductase reveal the molecular basis by which substrate preference is modulated by a distal site on the enzyme.
Light absorption by the algal transcription factor Aureochrome 1a causes dimerization at the light-oxygen-voltage (LOV) sensing domain, which has implications for the design of synthetic photoreceptors for optogenetics.
Lara M Rangel, Jon W Rueckemann ... Howard Eichenbaum
Different rhythms uniquely contribute to task-related processing in the hippocampus, and changes in the rhythmic profile of the hippocampus reflect dynamic coordination of its cell activity.
The V600E mutation in BRAF is a cancer hot spot because it opens the activation segment through destabilization of autoinhibitory interactions, but it does not significantly impair folding of the inactive or active kinase domain.
The crystal structure of Pur-alpha in complex with DNA reveals its molecular mechanisms of nucleic-acid binding and unwinding, allowing for a better understanding of its essential role in neurons.
The RNA-binding protein PTBP1 is recruited to sites near stop codons in retroviral and human mRNAs, shielding them from detection and degradation by the nonsense-mediated mRNA decay pathway.
Corey M Dambacher, Evan J Worden ... Gabriel C Lander
Within the isolated lid sub-complex of the proteasome, a finely tuned network of interactions maintains the deubiquitinase in an inhibited conformation; dramatic rearrangements of the lid subunits upon incorporation into the holoenzyme lead to the deubiquitinase’s activation.
Bernadette Carroll, Dorothea Maetzel ... Viktor I Korolchuk
Signaling pathway mTOR can sense its principal activator amino acid arginine via a different mechanism than that previously described for other amino acids.
Douglas P Anderson, Dustin S Whitney ... Kenneth E Prehoda
Experimentally reconstructing the evolution of the molecular complex that animals use to orient the mitotic spindle establishes a simple genetic and physical mechanism for the emergence of a function essential for multicellularity.
Nup98-HoxA9 is recruited to Hox gene cluster regions together with the chromosomally pre-bound nuclear export factor Crm1, which induces aberrant expression of several Hox genes and affecting the differentiation of embryonic stem cells.
Measurements of transcript isoform specific translation across the human genome reveal that isoforms from the same gene can differ in protein production by two orders of magnitude.
The small GTPase Arf6 plays a role in setting the size of the readily releasable pool of synaptic vesicles and in the definition of the recycling route for endocytosed vesicles.
Andrew R Conery, Richard C Centore ... Robert J Sims III
CBP/EP300 bromodomain inhibitors have a therapeutic application in oncology via targeting the IRF4 transcriptional program, a clinically validated network critical for multiple myeloma cell viability.
Simon Mysling, Kristian Kølby Kristensen ... Michael Ploug
Intravascular triglyceride hydrolysis by lipoprotein lipase is crucial for delivering lipid nutrients to vital tissues, such as the heart, skeletal muscle, and adipose tissue.