January 2016

Research articles

    1. Computational and Systems Biology
    2. Neuroscience

    A stochastic neuronal model predicts random search behaviors at multiple spatial scales in C. elegans

    William M Roberts, Steven B Augustine ... Shawn R Lockery
    A stochastic model of locomotory control in C. elegans based on an extensive new set of tracking data can explain and predict effects of ablations and mutations on behavior.
    1. Cell Biology
    2. Computational and Systems Biology

    An analysis toolbox to explore mesenchymal migration heterogeneity reveals adaptive switching between distinct modes

    Hamdah Shafqat-Abbasi, Jacob M Kowalewski ... Staffan Strömblad
    “Discontinuous” and “Continuous” migration modes are divergent mesenchymal migration strategies that arise spontaneously in parallel in an equilibrium modulated by cell-matrix attachment and actomyosin contractility.
    1. Immunology and Inflammation

    TGFβ-dependent expression of PD-1 and PD-L1 controls CD8+ T cell anergy in transplant tolerance

    Marije Baas, Alix Besançon ... Sylvaine You
    The interplay between the cytokine TGFβ and the PD-1/PD-L1 pathway promotes CD8+ T cell anergy and tolerance towards transplanted tissues.
    1. Cell Biology
    2. Developmental Biology

    The beetle amnion and serosa functionally interact as apposed epithelia

    Maarten Hilbrant, Thorsten Horn ... Kristen A Panfilio
    Late morphogenesis of the insect extraembryonic epithelia involves transient formation of a basal-basal bilayer to coordinate the functional contributions of two discrete tissues.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Toxoplasma gondii peptide ligands open the gate of the HLA class I binding groove

    Curtis McMurtrey, Thomas Trolle ... William Hildebrand
    T. gondii infection alters how peptide ligands are presented to the immune system by inducing a previously unreported structural change in Human Leukocyte Antigens (HLAs).
    1. Computational and Systems Biology
    2. Chromosomes and Gene Expression

    Single-cell analysis of transcription kinetics across the cell cycle

    Samuel O Skinner, Heng Xu ... Ido Golding
    By properly accounting for gene copy number and cell-cycle effects, single cell snapshots of nascent and mature mRNA can be used to unveil the stochastic kinetics of gene activity.
    1. Structural Biology and Molecular Biophysics

    Mutational scanning reveals the determinants of protein insertion and association energetics in the plasma membrane

    Assaf Elazar, Jonathan Weinstein ... Sarel Jacob Fleishman
    Deep sequencing analysis quantifies the hydrophobicity of the plasma membrane and provides restraints for structure modeling of receptor homodimers to atomic precision.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Phosphoproteomics reveals that Parkinson's disease kinase LRRK2 regulates a subset of Rab GTPases

    Martin Steger, Francesca Tonelli ... Matthias Mann
    Discovery of a physiological LRRK2 substrate and a new mechanism of Rab regulation should aid Parkinson’s research and the understanding of Rab function.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin

    Marina Theodosiou, Moritz Widmaier ... Reinhard Fässler
    Kindlin-2 co-operates with talin to activate fibronectin-binding integrins on fibroblasts and subsequently induces cell spreading by recruiting paxillin to small, peripheral nascent adhesions.
    1. Developmental Biology

    Epigenetic regulation of hematopoiesis by DNA methylation

    Aniket V Gore, Brett Athans ... Brant M Weinstein
    Methylation of a key transcription factor gene by a de novo DNA methyltransferase assures the maintenance of hematopoietic stem cell fate.
    1. Stem Cells and Regenerative Medicine
    2. Chromosomes and Gene Expression

    An epigenetic switch ensures transposon repression upon dynamic loss of DNA methylation in embryonic stem cells

    Marius Walter, Aurélie Teissandier ... Déborah Bourc'his
    The various transposon families of the mammalian genome can adopt different types of repressive chromatin responses upon DNA methylation loss.
    1. Developmental Biology

    Maternally provided LSD1/KDM1A enables the maternal-to-zygotic transition and prevents defects that manifest postnatally

    Jadiel A Wasson, Ashley K Simon ... David J Katz
    Epigenetic reprogramming by LSD1/KDM1A acts at fertilization in mice to enable embryogenesis and prevent behavioural abnormalities.
    1. Structural Biology and Molecular Biophysics
    2. Microbiology and Infectious Disease

    Guanylate binding proteins directly attack Toxoplasma gondii via supramolecular complexes

    Elisabeth Kravets, Daniel Degrandi ... Klaus Pfeffer
    Cellular immunity against Toxoplasma parasites is orchestrated by GBP complexes attacking the parasite membrane.
    1. Developmental Biology
    2. Neuroscience

    Area-specific development of distinct projection neuron subclasses is regulated by postnatal epigenetic modifications

    Kawssar Harb, Elia Magrinelli ... Christian Alfano
    Lmo4 specifies two neuron subclasses in the mouse neocortex by promoting postnatal co-expression of the transcription factors Ctip2 and Satb2 via chromatin remodelling in a time and area-specific manner.
    1. Cell Biology
    2. Developmental Biology

    The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium

    Joaquim Grego-Bessa, Joshua Bloomekatz ... Kathryn V Anderson
    In addition to its role in regulating proliferation and cell death, the PTEN tumor suppressor regulates epithelial morphogenesis through the PDK1 kinase.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome

    Qianglin Fang, Ping Chen ... Rui-Ming Xu
    Distinct binding of viral proteins to the same region on the nucleosome surface can result in contrasting changes to higher-order chromatin structure in the host cell.
    1. Stem Cells and Regenerative Medicine
    2. Immunology and Inflammation

    MHC-compatible bone marrow stromal/stem cells trigger fibrosis by activating host T cells in a scleroderma mouse model

    Yoko Ogawa, Satoru Morikawa ... Shigeto Shimmura
    PDGFRα+ Sca-1+ bone marrow stromal/stem cells in whole bone marrow grafts can trigger the onset of autoimmune-related fibrosis in a mouse model of scleroderma.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Population genomics reveals the origin and asexual evolution of human infective trypanosomes

    William Weir, Paul Capewell ... Annette MacLeod
    The protozoan parasite Trypanosoma brucei gambiense has undergone recent clonal evolution that reveals the theoretically predicted Meselson effect at a genome-wide level.
    1. Genetics and Genomics
    2. Neuroscience

    Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay

    Myungjin Kim, Erin Sandford ... Margit Burmeister
    A genetic mutation that impairs autophagic flux leads to neurodegeneration and can cause ataxia and developmental delay in children.
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Photo-switchable tweezers illuminate pore-opening motions of an ATP-gated P2X ion channel

    Chloé Habermacher, Adeline Martz ... Thomas Grutter
    The gating mechanism of trimeric ATP-gated P2X receptors has been explored using photo-switchable cross-linkers, via a versatile strategy that could be applied to other membrane proteins.
    1. Developmental Biology

    The ‘de novo’ DNA methyltransferase Dnmt3b compensates the Dnmt1-deficient intestinal epithelium

    Ellen N Elliott, Karyn L Sheaffer, Klaus H Kaestner
    The maintenance methyltransferase Dnmt1 and the de novo methyltransferase Dnmt3b cooperate to maintain DNA methylation and genomic stability in the adult intestinal epithelium.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Trogocytosis-associated cell to cell spread of intracellular bacterial pathogens

    Shaun Steele, Lauren Radlinski ... Thomas H Kawula
    Live intracellular bacterial pathogens can transfer from infected cells to uninfected macrophages upon cell-to-cell contact.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Unnatural amino acid photo-crosslinking of the IKs channel complex demonstrates a KCNE1:KCNQ1 stoichiometry of up to 4:4

    Christopher I Murray, Maartje Westhoff ... David Fedida
    The IKs potassium channel complex displays functional flexibility that depends on the ratio of its constituent KCNQ1 and KCNE1 protein subunits.
    1. Cell Biology
    2. Neuroscience

    Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function

    Peer-Hendrik Kuhn, Alessio Vittorio Colombo ... Stefan F Lichtenthaler
    The metalloprotease ADAM10 modulates axon and synapse function by cleaving numerous synaptic and axonal membrane proteins in the central nervous system.
    1. Structural Biology and Molecular Biophysics

    Revealing an outward-facing open conformational state in a CLC Cl/H+ exchange transporter

    Chandra M Khantwal, Sherwin J Abraham ... Merritt Maduke
    The characterization of a previously unidentified “outward-facing open” conformational state provides a new framework for understanding the CLC transport mechanism.
    1. Cell Biology

    Transmembrane chemokines act as receptors in a novel mechanism termed inverse signaling

    Kirsten Hattermann, Henrike Gebhardt ... Rolf Mentlein
    In a new mechanism of cellular communication termed “inverse signaling", a transmembrane chemokine acts as a "receptor" for its previously shed form.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Eighteenth century Yersinia pestis genomes reveal the long-term persistence of an historical plague focus

    Kirsten I Bos, Alexander Herbig ... Hendrik N Poinar
    The analysis of 18th century Y. pestis genomes reveals a bacterial lineage that might be responsible for the 400-year period of European plague epidemics from the Renaissance through early modern times.
    1. Neuroscience

    A platform for brain-wide imaging and reconstruction of individual neurons

    Michael N Economo, Nathan G Clack ... Jayaram Chandrashekar
    High-resolution fluorescence imaging of the complete mouse brain enables many neurons to be efficiently visualized in their entirety, revealing all targets of neurons that project widely across the brain.
    1. Cell Biology
    2. Developmental Biology

    Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity

    Bart-Jan de Kreuk, Alexandre R Gingras ... Mark H Ginsberg
    The proteins Heart of Glass (HEG1) and Rasip1 are both essential for cardiovascular development; and directly bind to each other to guide Rasip1 to endothelial cell junctions to support vascular integrity.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular mechanism of thermosensory function of human heat shock transcription factor Hsf1

    Nikolai Hentze, Laura Le Breton ... Matthias P Mayer
    Heat-induced local unfolding allows Hsf1 to form trimers and bind to DNA, which depends on Hsf1 concentration and is promoted, not inhibited, by Hsp90.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Myosin III-mediated cross-linking and stimulation of actin bundling activity of Espin

    Haiyang Liu, Jianchao Li ... Mingjie Zhang
    The tail domain of Myosin III binds to and cross-links actin bundling protein Espin1 and thus modulates higher order actin bundle structures in cellular processes such as stereocilia and microvilli.
    1. Neuroscience

    The cerebellum linearly encodes whisker position during voluntary movement

    Susu Chen, George J Augustine, Paul Chadderton
    Cerebellar Purkinje cells represent movements via bidirectional linear changes in spike rate, and activity from single cells is sufficient to reconstruct kinematic changes during bouts of free whisking.
    1. Biochemistry and Chemical Biology
    2. Neuroscience

    A chemical proteomic atlas of brain serine hydrolases identifies cell type-specific pathways regulating neuroinflammation

    Andreu Viader, Daisuke Ogasawara ... Benjamin F Cravatt
    Cell-specific pathways regulate lipid transmitters and neuroinflammation in the brain.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The magnesium transporter A is activated by cardiolipin and is highly sensitive to free magnesium in vitro

    Saranya Subramani, Harmonie Perdreau-Dahl, Jens Preben Morth
    A magnesium transporter from Escherichia coli depends strongly on the lipid cardiolipin and acts both as a sensor and a transporter of magnesium.
    1. Immunology and Inflammation

    ILC3 GM-CSF production and mobilisation orchestrate acute intestinal inflammation

    Claire Pearson, Emily E Thornton ... Fiona Powrie
    Innate lymphoid cells, which are dynamic under steady-state conditions, respond to a colitogenic stimulus by mobilizing from cryptopatches and secreting GM-CSF to organize the pro-inflammatory response.
    1. Developmental Biology

    Position-dependent plasticity of distinct progenitor types in the primitive streak

    Filip J Wymeersch, Yali Huang ... Valerie Wilson
    Neuromesodermal and lateral/ventral mesoderm progenitors represent distinct committed states, governed by separate regulatory logics.
    1. Neuroscience

    Motion along the mental number line reveals shared representations for numerosity and space

    Caspar M Schwiedrzik, Benjamin Bernstein, Lucia Melloni
    Looking at visual motion affects perception of nonsymbolic numerosity in a direction-specific way, indicating that motion and number are computed by the same neurons.
    1. Structural Biology and Molecular Biophysics

    α/β coiled coils

    Marcus D Hartmann, Claudia T Mendler ... Birte Hernandez Alvarez
    Simple insertions switch α-helical coiled coils into a novel fibrous protein fold that is periodically interspersed with short β-strands.
    1. Developmental Biology

    Genetic dissection of Down syndrome-associated congenital heart defects using a new mouse mapping panel

    Eva Lana-Elola, Sheona Watson-Scales ... Victor LJ Tybulewicz
    A panel of seven new mouse strains with chromosomal duplications is used to identify a minimal genetic region required in three copies to cause congenital heart defects typical of human Down syndrome.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

    Brian C Richardson, Steve L Halaby ... J Christopher Fromme
    A new structure reveals two Arf-GEF regulatory domains form a single structural unit that stimulates Arf-GEF activity.
    1. Computational and Systems Biology
    2. Chromosomes and Gene Expression

    NF-κB oscillations translate into functionally related patterns of gene expression

    Samuel Zambrano, Ilario De Toma ... Alessandra Agresti
    NF-κB oscillations synchronize to external perturbations as a damped oscillator, producing different transcription dynamics.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution

    Scott M Coyle, Wendell A Lim
    A systems level reconstitution of H-Ras GTPase signaling reveals a rich space of tunable dynamic outputs and clarifies the consequences of oncogenic perturbations to signaling.
    1. Microbiology and Infectious Disease
    2. Plant Biology

    An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor

    Yasin F Dagdas, Khaoula Belhaj ... Tolga O Bozkurt
    A plant pathogen effector evolved a short linear motif to subvert autophagic defenses by antagonising a host cargo receptor.
    1. Developmental Biology

    An oxygen-insensitive Hif-3α isoform inhibits Wnt signaling by destabilizing the nuclear β-catenin complex

    Peng Zhang, Yan Bai ... Cunming Duan
    Hif/Hif-3α has two distinct roles in cells: activating HRE-dependent gene expression and binding to β-catenin to destabilize the nuclear β-catenin complex.
    1. Cell Biology
    2. Developmental Biology

    NuMA-microtubule interactions are critical for spindle orientation and the morphogenesis of diverse epidermal structures

    Lindsey Seldin, Andrew Muroyama, Terry Lechler
    A newly identified microtubule binding activity of NuMA is required for orienting the mitotic spindle.
    1. Cell Biology
    2. Computational and Systems Biology

    Evaluation of predictions of the stochastic model of organelle production based on exact distributions

    C Jeremy Craven
    A reanalysis of the stochastic model of organelle production (Mukherji and O'Shea, 2014) suggests that this model requires significant further discussion.
    1. Neuroscience

    IK1 channels do not contribute to the slow afterhyperpolarization in pyramidal neurons

    Kang Wang, Pedro Mateos-Aparicio ... John P Adelman
    The slow afterhyperpolarization that follows a burst of action potentials is a powerful regulator of neuronal excitability and is not due to IK1 (KCNN4), a member of the SK channel family.
    1. Cell Biology
    2. Computational and Systems Biology

    Bistability of a coupled Aurora B kinase-phosphatase system in cell division

    Anatoly V Zaytsev, Dario Segura-Peña ... Ekaterina L Grishchuk
    Self-activating Aurora B kinase, opposed by an inhibitory phosphatase, forms spatial phosphorylation patterns during cell division.
    1. Cancer Biology
    2. Cell Biology

    Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis

    Sandra Kümper, Faraz K Mardakheh ... Christopher J Marshall
    The Rho kinases ROCK1 and 2 act redundantly in regulating cell proliferation and their depletion causes cellular senescence.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Mapping translation 'hot-spots' in live cells by tracking single molecules of mRNA and ribosomes

    Zachary B Katz, Brian P English ... Robert H Singer
    A method that involves simultaneous tracking of individual mRNAs and their associated ribosomes can be used to determine when and where individual molecules get translated in living cells.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular basis for allosteric specificity regulation in class Ia ribonucleotide reductase from Escherichia coli

    Christina M Zimanyi, Percival Yang-Ting Chen ... Catherine L Drennan
    Substrate and effector bound structures of a bacterial ribonucleotide reductase reveal the molecular basis by which substrate preference is modulated by a distal site on the enzyme.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Blue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence

    Udo Heintz, Ilme Schlichting
    Light absorption by the algal transcription factor Aureochrome 1a causes dimerization at the light-oxygen-voltage (LOV) sensing domain, which has implications for the design of synthetic photoreceptors for optogenetics.
    1. Chromosomes and Gene Expression

    Glutaminase 2 is a novel negative regulator of small GTPase Rac1 and mediates p53 function in suppressing metastasis

    Cen Zhang, Juan Liu ... Zhaohui Feng
    The GLS2 enzyme suppresses cancer metastasis by interacting with the small GTPase Rac1 and inhibiting its activity.
    1. Neuroscience

    Rhythmic coordination of hippocampal neurons during associative memory processing

    Lara M Rangel, Jon W Rueckemann ... Howard Eichenbaum
    Different rhythms uniquely contribute to task-related processing in the hippocampus, and changes in the rhythmic profile of the hippocampus reflect dynamic coordination of its cell activity.
    1. Cell Biology
    2. Developmental Biology

    Local mechanical forces promote polarized junctional assembly and axis elongation in Drosophila

    Jessica C Yu, Rodrigo Fernandez-Gonzalez
    Polarized assembly of cell-cell junctions drives Drosophila axis elongation.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    The yin–yang of kinase activation and unfolding explains the peculiarity of Val600 in the activation segment of BRAF

    Christina Kiel, Hannah Benisty ... Luis Serrano
    The V600E mutation in BRAF is a cancer hot spot because it opens the activation segment through destabilization of autoinhibitory interactions, but it does not significantly impair folding of the inactive or active kinase domain.
    1. Developmental Biology

    The REST remodeling complex protects genomic integrity during embryonic neurogenesis

    Tamilla Nechiporuk, James McGann ... Gail Mandel
    The untimely loss of the transcriptional repressor REST from dividing neural progenitors during embryogenesis leads to compromised genomic integrity.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural basis of nucleic-acid recognition and double-strand unwinding by the essential neuronal protein Pur-alpha

    Janine Weber, Han Bao ... Dierk Niessing
    The crystal structure of Pur-alpha in complex with DNA reveals its molecular mechanisms of nucleic-acid binding and unwinding, allowing for a better understanding of its essential role in neurons.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Polypyrimidine tract binding protein 1 protects mRNAs from recognition by the nonsense-mediated mRNA decay pathway

    Zhiyun Ge, Bao Lin Quek ... J Robert Hogg
    The RNA-binding protein PTBP1 is recruited to sites near stop codons in retroviral and human mRNAs, shielding them from detection and degradation by the nonsense-mediated mRNA decay pathway.
    1. Structural Biology and Molecular Biophysics

    Atomic structure of the 26S proteasome lid reveals the mechanism of deubiquitinase inhibition

    Corey M Dambacher, Evan J Worden ... Gabriel C Lander
    Within the isolated lid sub-complex of the proteasome, a finely tuned network of interactions maintains the deubiquitinase in an inhibited conformation; dramatic rearrangements of the lid subunits upon incorporation into the holoenzyme lead to the deubiquitinase’s activation.
    1. Cell Biology

    Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity

    Bernadette Carroll, Dorothea Maetzel ... Viktor I Korolchuk
    Signaling pathway mTOR can sense its principal activator amino acid arginine via a different mechanism than that previously described for other amino acids.
    1. Cell Biology
    2. Evolutionary Biology

    Evolution of an ancient protein function involved in organized multicellularity in animals

    Douglas P Anderson, Dustin S Whitney ... Kenneth E Prehoda
    Experimentally reconstructing the evolution of the molecular complex that animals use to orient the mitotic spindle establishes a simple genetic and physical mechanism for the emergence of a function essential for multicellularity.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes

    Masahiro Oka, Sonoko Mura ... Yoshihiro Yoneda
    Nup98-HoxA9 is recruited to Hox gene cluster regions together with the chromosomally pre-bound nuclear export factor Crm1, which induces aberrant expression of several Hox genes and affecting the differentiation of embryonic stem cells.
    1. Cell Biology
    2. Neuroscience

    Dendritic mitochondria reach stable positions during circuit development

    Michelle C Faits, Chunmeng Zhang ... Daniel Kerschensteiner
    Mitochondria reach stable positions in dendrites during a critical developmental period of high motility.
    1. Computational and Systems Biology
    2. Chromosomes and Gene Expression

    Tunable protein synthesis by transcript isoforms in human cells

    Stephen N Floor, Jennifer A Doudna
    Measurements of transcript isoform specific translation across the human genome reveal that isoforms from the same gene can differ in protein production by two orders of magnitude.
    1. Neuroscience

    Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse

    Erica Tagliatti, Manuela Fadda ... Anna Fassio
    The small GTPase Arf6 plays a role in setting the size of the readily releasable pool of synaptic vesicles and in the definition of the recycling route for endocytosed vesicles.
    1. Chromosomes and Gene Expression
    2. Cancer Biology

    Bromodomain inhibition of the transcriptional coactivators CBP/EP300 as a therapeutic strategy to target the IRF4 network in multiple myeloma

    Andrew R Conery, Richard C Centore ... Robert J Sims III
    CBP/EP300 bromodomain inhibitors have a therapeutic application in oncology via targeting the IRF4 transcriptional program, a clinically validated network critical for multiple myeloma cell viability.
    1. Cell Biology
    2. Neuroscience

    The p38 MAP kinase pathway modulates the hypoxia response and glutamate receptor trafficking in aging neurons

    Eun Chan Park, Christopher Rongo
    The subcellular localization of the prolyl hydroxylase oxygen sensor in C. elegans neurons is regulated by p38 MAP kinase signaling.
    1. Structural Biology and Molecular Biophysics

    The acidic domain of the endothelial membrane protein GPIHBP1 stabilizes lipoprotein lipase activity by preventing unfolding of its catalytic domain

    Simon Mysling, Kristian Kølby Kristensen ... Michael Ploug
    Intravascular triglyceride hydrolysis by lipoprotein lipase is crucial for delivering lipid nutrients to vital tissues, such as the heart, skeletal muscle, and adipose tissue.