October 2025

Cover articles

    1. Plant Biology

    The evolution of effectors

    Soham Mukhopadhyay, Muhammad Asim Javed ... Edel Perez-Lopez
    1. Neuroscience

    The safety-efficiency dilemma

    Pranav Mahajan, Shuangyi Tong ... Ben Seymour
    1. Developmental Biology

    Tracing cell fates in embryos

    Ying Zhang, Qi Chen

  4. New university partnerships

Highlights controls:

Research articles

    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformation

    Takashi Hotta, Morgan L Pimm ... Kristen J Verhey
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Compelling
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Unbend: Correction of local beam-induced sample motion in cryo-EM images using a 3D spline model

    Lingli Kong, Ximena Zottig ... Nikolaus Grigorieff
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Genetics and Genomics

    Mutational and Expression Profile of ZNF217, ZNF750, ZNF703 Zinc Finger Genes in Kenya Women diagnosed with Breast Cancer

    Michael Kitoi, John Gitau ... Francis Makokha
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Evolutionary Biology

    Earliest Evidence of Elephant Butchery at Olduvai Gorge (Tanzania) Reveals the Evolutionary Impact of Early Human Megafaunal Exploitation

    Manuel Domínguez-Rodrigo, Enrique Baquedano ... Audax Mabulla
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Genetics and Genomics

    Monocyte-endothelial interactions as a targetable node in clonal hematopoiesis-mediated cardiovascular disease

    Alyssa C Parker, J Brett Heimlich ... Alexander G Bick
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    A stochastic RNA editing process targets a select number of sites in individual Drosophila glutamatergic motoneurons

    Andrés B Crane, Michiko O Inouye ... J Troy Littleton
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology

    A Novel 3D Visualization Method in Mice Identifies the Periportal Lamellar Complex (PLC) as a Key Regulator of Hepatic Ductal and Neuronal Branching Morphogenesis

    Tongtong Xu, Fujun Cao ... Chengjian Zhao
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Region-specific mechanosensation controls Drosophila postural control behaviour

    William Roseby, Jonathan AC Menzies ... Claudio R Alonso
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    • Incomplete
    1. Genetics and Genomics

    Redox Dyshomeostasis Links Renal and Neuronal Dysfunction in Drosophila Models of Gaucher and Parkinson’s Disease

    Alexander J Hull, Magda L Atilano, Kerri J Kinghorn
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Biochemistry and Chemical Biology
    2. Immunology and Inflammation

    Structure-based Design of Chimeric Influenza Hemagglutinins to Elicit Cross-group Immunity

    Karla M Castro, Reyhaneh Ayardulabi ... Bruno E Correia
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Microbiology and Infectious Disease

    Research advance: Unexpected plasticity in the life cycle of Trypanosoma brucei

    Carina Praisler, Jaime N Lisack ... Markus Engstler
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Solid
    1. Cell Biology
    2. Genetics and Genomics

    Mechanistic insights into transcriptional regulation of ARHGAP36 expression identify a factor predictive of neuroblastoma survival

    Serhiy Havrylov, Armin M Gamper, Ordan J Lehmann
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Cell Biology

    Abnormal shear stress induces ferroptosis in endothelial cells via KLF6 downregulation

    Jingang Cui, Zhiyu Fan ... Yongsheng Ding
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Cryo-electron tomography reveals the microtubule-bound form of inactive LRRK2

    Siyu Chen, Tamar Basiashvili ... Elizabeth Villa
    Full-length, autoinhibited LRRK2 assembles into distinct, shorter microtubule-bound filaments via a previously unobserved N-terminal repeat interface, revealing new structural states with potential therapeutic implications.
    Version of Record
    Short Report
    • Important
    • Convincing
    • Incomplete
    1. Cell Biology

    Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis

    Davinder Singh, Yajun Liu ... Jian-Qiu Wu
    Protein–protein interactions and localization dependencies between septin cytoskeleton and the exocyst complex using the fission yeast model system reveal one of the most conserved functions of septins in exocytosis.
    1. Microbiology and Infectious Disease
    2. Computational and Systems Biology

    Automated genome mining predicts structural diversity and taxonomic distribution of peptide metallophores across bacteria

    Zachary L Reitz, Bita Pourmohsenin ... Marnix H Medema
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Neuroscience

    Dissecting surveying behavior of reactive microglia under chronic neurodegeneration

    Sunitha Subhramanian, Olga Bocharova ... Ilia V Baskakov
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Inhibitory columnar feedback neurons are required for motion processing in Drosophila

    Miriam Henning, Madhura Ketkar ... Marion Silies
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Structural Biology and Molecular Biophysics

    Cryo-EM Reveals Regulatory Mechanisms Governing Substrate Selection and Activation of Human LONP1

    Jeffrey T Mindrebo, Lauren Alexandrescu ... Gabriel C Lander
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Dissociation of the nuclear basket triggers chromosome loss in aging yeast

    Mihailo Mirkovic, Jordan McCarthy ... Yves Barral
    Aging results in remodeling of the nuclear pore complex and leakage of intron-containing pre-mRNA out of the nucleus, altering gene function and mitotic stability of old cells.
    1. Computational and Systems Biology

    OpenSpliceAI provides an efficient modular implementation of SpliceAI enabling easy retraining across nonhuman species

    Kuan-Hao Chao, Alan Mao ... Mihaela Pertea
    OpenSpliceAI is an open, retrainable framework for splice site prediction that enables rapid, memory-efficient, cross-species analyses at scale with accuracy comparable to SpliceAI.
    1. Plant Biology

    Companion cells with high florigen production express other small proteins and reveal a nitrogen-sensitive FT repressor

    Hiroshi Takagi, Shogo Ito ... Takato Imaizumi
    A distinct subset of leaf companion cells expresses high levels of the florigen gene, and the same cells also produce other possible mobile floral regulators and small proteins.
    1. Neuroscience
    2. Computational and Systems Biology

    Where is the melody? Spontaneous attention orchestrates melody formation during polyphonic music listening

    Martin M Winchester, Kevin Reynolds ... Giovanni M Di Liberto
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Megabouts: a flexible pipeline for zebrafish locomotion analysis

    Adrien Jouary, Pedro TM Silva ... Michael B Orger
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Plant Biology

    Herbivorous insects independently evolved salivary effectors to regulate plant immunity by destabilizing the malectin-LRR RLP NtRLP4

    Xin Wang, Jia-Bao Lu ... Hai-Jian Huang
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Asymmetric neural entrainment at resonance frequencies underlies unilateral spatial neglect

    Yuka O Okazaki, Noriaki Hattori ... Keiichi Kitajo
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Microbiology and Infectious Disease

    Detecting, mapping, and suppressing the spread of a decade-long Pseudomonas aeruginosa nosocomial outbreak with genomics

    William Stribling, Lindsey R Hall ... Francois Lebreton
    Routine genomic surveillance uncovered a decades-long multidrug-resistant Pseudomonas aeruginosa outbreak sustained by patient reservoirs and hospital plumbing, enabling targeted infection control measures that ultimately curtailed transmission.
    1. Neuroscience

    Axonal injury signaling is restrained by a spared synaptic branch

    Laura J Smithson, Juliana L Zang ... Catherine A Collins
    Transcriptional responses to axonal damage are required for circuit repair, but these responses are restrained in injuries that leave spared axonal branches.
    1. Computational and Systems Biology
    2. Physics of Living Systems

    Non-equilibrium strategies enabling ligand specificity by signaling receptors

    Andrew Goetz, Jeremy Barrios ... Purushottam D Dixit
    Non-equilibrium thermodynamics allows signaling networks to signal downstream of certain ligands but avoid signaling from others.
    1. Neuroscience

    Dual-format attentional template during preparation in human visual cortex

    Yilin Chen, Taosheng Liu ... Mengyuan Gong
    Preparatory attentional templates exist in two formats with distinct functional states, a default non-sensory format and a latent sensory-like format.
    1. Neuroscience

    A stress-activated neuronal ensemble in the supramammillary nucleus encodes anxiety but not memory

    Jinming Zhang, Kexin Yu ... Jing Han
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    • Incomplete
    1. Cell Biology

    The Rab7-Epg5 and Rab39-ema modules cooperatively position autophagosomes for efficient lysosomal fusions

    Attila Boda, Villő Balázs ... Péter Lőrincz
    An RNAi based genetic screen and subsequent analyses reveal how dyneins and small GTPases coordinate autophagosome positioning at the ncMTOC in fat cells, advancing understanding of autophagic vesicle transport.
    1. Computational and Systems Biology
    2. Medicine

    Critique of impure reason: Unveiling the reasoning behaviour of medical large language models

    Shamus Zi Yang Sim, Tyrone Chen
    A survey of reasoning behaviour in medical large language models uncovers emerging trends, highlights open challenges, and introduces theoretical frameworks that enhance reasoning behaviour transparency, ultimately fostering greater trust among clinicians, developers, and patients in their deployment.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Changes in neural progenitor lineage composition during astrocytic differentiation of human iPSCs

    Zongze Li, Lucia Fernandez Cardo ... Meng Li
    A genetic tracing study demonstrates that neural progenitor lineage dominance may not be maintained in differentiated progeny, underscoring the critical need for region-specific markers to validate pluripotent stem cell–derived products.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner

    Yoshihisa Kaizuka, Rika Machida
    Intermolecular steric repulsion generated by glycans on the cell membrane inhibits viral infection by preventing the formation of a virus-cell interface, regardless of the identity of molecules for glycan modification.
    1. Neuroscience

    Neuronal migration depends on blood flow in the adult mammalian brain

    Takashi Ogino, Akari Saito ... Kazunobu Sawamoto
    Blood flow enhances neuronal migration in the adult brain via ghrelin signaling.
    1. Neuroscience

    Nocebo effects are stronger and more persistent than placebo effects in healthy individuals

    Angelika Kunkel, Katharina Schmidt ... Ulrike Bingel
    In healthy volunteers, negative expectations exert stronger and longer-lasting effects on pain than positive expectations, consistent with a better-safe-than-sorry account of placebo and nocebo effects.
    1. Epidemiology and Global Health

    Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions

    Kathryn E Tiedje, Qi Zhan ... Karen P Day
    Despite major perturbations from sequential interventions, the Plasmodium falciparum population remained very large and retained the var population genetic characteristics of a high-transmission system.
    1. Neuroscience

    Newly trained navigation and verbal memory skills in humans elicit changes in task-related networks but not brain structure

    Li Zheng, Zachary Boogaart ... Steven M Weisberg
    Learning new navigation or memory skills engages flexible brain network dynamics without altering gray matter, white matter, or hippocampal structure.
    1. Cell Biology

    Deep3DSIM: Super-resolution imaging of thick tissue using 3D structured illumination with adaptive optics

    Jingyu Wang, Danail Stoychev ... Ilan Davis
    Deep3DSIM enables super-resolution imaging >130 µm into complex tissues of fixed and live specimens in an upright configuration, allowing sample manipulation, as well as rapid refocusing without the need to move the specimen or objective lens.
    1. Developmental Biology
    2. Genetics and Genomics

    Gene regulatory dynamics during craniofacial development in a carnivorous marsupial

    Laura E Cook, Charles Y Feigin ... Irene Gallego Romero
    While the genes involved in craniofacial development are highly conserved between the fat-tailed dunnart and mouse, their cis-regulatory elements show significant divergence and appear to reflect species-specific developmental processes.
    1. Developmental Biology
    2. Cancer Biology

    Tumors mimic the niche to inhibit neighboring stem cell differentiation

    Yang Zhang, Yuejia Wang ... Shaowei Zhao
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Chromosomes and Gene Expression

    Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events

    Susan Wang, Thomas Suter ... Michael G Rosenfeld
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Tonotopy is not preserved in a descending stage of auditory cortex

    Miaoqing Gu, Shanshan Liang ... Xiaowei Chen
    Cortical feedback to the thalamus lacks the precise spatial organization seen in ascending pathways.
    1. Biochemistry and Chemical Biology

    Brown adipose tissue and skeletal muscle coordinately contribute to thermogenesis in mice

    Yuna Izumi-Mishima, Rie Tsutsumi ... Hiroshi Sakaue
    Muscle-derived branched-chain amino acids support thermogenesis in brown adipose tissue, thereby helping maintain body temperature during impaired muscle function or physiological stress.
    1. Evolutionary Biology

    The holocephalan ratfish endoskeleton shares trabecular and areolar mineralization patterns, but not tesserae, with elasmobranchs little skate and catshark

    Oghenevwogaga Joseph Atake, Fidji Berio ... B Frank Eames
    Novel and published data confirm that cartilaginous fishes (chondrichthyans) from many different clades share interesting skeletal features that are similar to bone, but some 'classic' chondrichthyan features are not shared.
    1. Neuroscience

    Cerebellar climbing fibers impact experience-dependent plasticity in the mouse primary somatosensory cortex

    Abby Silbaugh, Kevin P Koster, Christian Hansel
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Convincing
    1. Medicine
    2. Neuroscience

    Functional Muscle Networks as Biomarkers of Post-Stroke Motor Impairment and Therapeutic Responsiveness

    David O’Reilly, Giorgia Pregnolato ... Giacomo Severini
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Developmental Biology
    2. Computational and Systems Biology

    Anti-resonance in developmental signaling regulates cell fate decisions

    Samuel J Rosen, Olivier Witteveen ... Maxwell Z Wilson
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Genetics and Genomics

    Rewiring the bone marrow: Evolution and the transcriptional architecture of trained immunity

    Sarah J Sun, Raúl Aguirre-Gamboa, Luis B Barreiro
    Hematopoietic reprogramming reflects the selective and evolutionarily conserved engagement of transcription factor networks that encode innate immune memory in long-lived stem cells.
    1. Neuroscience

    Single-cell transcriptome sequencing for opening the blood-brain barrier through specific mode electroacupuncture stimulation

    Congcong Ma, Zhaoxing Jia ... Xianming Lin
    Single-cell transcriptome sequencing results of rats intervened with specific mode electroacupuncture (an emerging method for opening the blood-brain barrier).
    1. Epidemiology and Global Health

    A natural experiment in Kenya reveals durable immunosuppressive effects of early childhood malaria: a longitudinal cohort study

    Mercy S Safari, Timothy O Makori ... Charles J Sande
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Structural Biology and Molecular Biophysics

    Molecular Architecture and Function Mechanism of Tri-heteromeric GluN1-N2-N3A NMDA Receptors

    Zengwei Kou, Fenyong Yao ... Yidi Sun
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    The Crunchometer: A Low-Cost, Open-Source Acoustic Analysis of Feeding Microstructure

    Elvi Gil-Lievana, Benjamin Arroyo ... Ranier Gutierrez
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    • Incomplete
    1. Developmental Biology
    2. Evolutionary Biology

    Molecular dynamics of the matrisome across sea anemone life history

    Bruno Gideon Bergheim, Alison G Cole ... Suat Özbek
    Integrated proteomic and single-cell transcriptomic analyses define the Nematostella vectensis matrisome and reveal conserved extracellular matrix functions driving life cycle transitions in this non-bilaterian model.
    1. Neuroscience

    Spatiotemporal brain complexity quantifies consciousness outside of perturbation paradigms

    Martin Breyton, Jan Fousek ... Viktor Jirsa
    Brain fluidity metrics provide an accessible and reliable marker of consciousness, offering a practical alternative to perturbational complexity for clinical and research applications.
    1. Physics of Living Systems

    Toward stable replication of genomic information in pools of RNA molecules

    Ludwig Burger, Ulrich Gerland
    Theoretical and computational analyses reveal environmental, compositional, kinetic, and thermodynamic constraints on a scenario where sequence information collectively stored in pools of RNA molecules is replicated via non-enzymatic RNA ligation.
    1. Evolutionary Biology

    Elevated heterozygosity clued atypical reproduction: evidence consistent with hybridogenesis mediating insular occurrence of a narrowly endemic spiny frog

    Yuchi Zheng, Bin Wang ... Yun Xia
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Neuroscience

    Heterogeneous responses to embryonic critical period perturbations within the Drosophila larval locomotor circuit

    Niklas Krick, Jacob Davies ... Matthias Landgraf
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Twice as nice: Boosts in adolescent reinforcement learning from Pavlovian bias and age-related prioritization of reward-motivated incidental memory

    Haley Hegefeld, Juliet Y Davidow
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Developmental Biology

    Cataloguing the postnatal small intestinal transcriptome during the first postnatal month

    Luiz Fernando Silva Oliveira, Radhika S Khetani ... Amy E O’Connell
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    • Incomplete
    1. Neuroscience

    The view-tolerance of human identity recognition depends on horizontal face information

    Alexia Roux-Sibilon, Hélène Dumont ... Valérie Goffaux
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Time-adaptive modulation of evidence evaluation in rat posterior parietal cortex

    Preetham Ganupuru, Adam B Goldring ... Timothy D Hanks
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience
    2. Physics of Living Systems

    A stretching mechanism evokes mechano-electrical transduction in auditory chordotonal neurons

    Atitheb Chaiyasitdhi, Manuela Nowotny ... Benjamin Warren
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    • Incomplete
    1. Cell Biology

    Suppression of interferon signaling via small molecule modulation of TFAM

    Dionisia P Sideris, Hsuan Lee ... Lin Lyu
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Stem Cells and Regenerative Medicine

    Chromatin activity of IκBα mediates the exit from naïve pluripotency

    Luis G Palma, Daniel Alvarez-Villanueva ... Anna Bigas
    The development of novel separation-of-function mutants has uncovered non-canonical chromatin roles of IκBα in regulating mouse embryonic stem cell pluripotency and differentiation, independently of the classical NF-κB pathway.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    IFIT1 is rapidly evolving and exhibits disparate antiviral activities across 11 mammalian orders

    Matthew B McDougal, Ian N Boys ... John W Schoggins
    Evolutionary changes in RNA binding and antiviral activity result in diverse IFIT1 functions across mammals, even among closely related species.
    1. Neuroscience

    Longitudinal assessment of DREADD expression and efficacy in the monkey brain

    Yuji Nagai, Yukiko Hori ... Takafumi Minamimoto
    Designer Receptors Exclusively Activated by Designer Drug (DREADD) expression in macaque brains peaks around 2 months after adeno-associated virus injection and remains stable for over a year before gradually declining.
    1. Stem Cells and Regenerative Medicine

    Stabilisation of HIF signalling in the mouse epicardium extends embryonic potential and neonatal heart regeneration

    Elisabetta Gamen, Eleanor L Price ... Paul R Riley
    Extending hypoxia signalling after birth extends the window of heart regeneration.
    1. Neuroscience

    Blood pressure variability compromises vascular function in middle-aged mice

    Perenkita J Mendiola, Philip O'Herron ... Jessica A Filosa
    Chronic variations in blood pressure compromise brain health.
    1. Neuroscience

    Sequential temporal anticipation characterized by neural power modulation and in recurrent neural networks

    Xiangbin Teng, Ru-Yuan Zhang
    Predicting key future moments requires ignoring most irrelevant time, and alpha and beta power modulations encode sequential temporal landmarks via a gain-control mechanism that suppresses these irrelevant periods.
    1. Neuroscience

    CellCover Defines Marker Gene Panels Capturing Developmental Progression in Neocortical Neural Stem Cell Identity

    Lanlan Ji, An Wang ... Donald Geman
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    1. Neuroscience

    The nucleus accumbens shell regulates hedonic feeding via a rostral hotspot

    Alina-Măriuca Marinescu, Eshita Kamal ... Marie A Labouesse
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Developmental Biology

    Spatiotemporal WNT and BMP gradients orchestrate regional enteroendocrine cell diversity along the Drosophila midgut

    Jiaying Lv, Xingting Guo, Rongwen Xi
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Chromosomes and Gene Expression

    Mediator Subunit MED16 Collaborates with UBP1-TFCP2 to Control Transcriptional Activation or Repression via Promoter Positional Specificity

    Yuanming Zheng, Xiaying Zhao ... Gang Wang
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Genetics and Genomics
    2. Plant Biology

    The genetic control of rapid genome content divergence in Arabidopsis thaliana

    Christopher J Fiscus, Daniel Koenig
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Genetics and Genomics

    Abundant Parent-of-origin Effect eQTL: The Framingham Heart Study

    Yongtao Guan, Tianxiao Huan, Daniel Levy
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Genetics and Genomics

    Adapting Clinical Chemistry Plasma as a Source for Liquid Biopsies

    Spencer C Ding, Jingru Yu ... Wei Gu
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Bilateral equalization of synaptic output in olfactory glomeruli of Xenopus tadpoles

    Marta Casas, Beatrice Terni, Artur Llobet
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Aggressive trait selection influences life-history trade-offs, favoring survival over reproductive success in Drosophila melanogaster males

    Anthony Defert, Romane Gout ... Séverine Trannoy
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Two neuropeptides that promote blood-feeding in Anopheles stephensi mosquitoes

    Prashali Bansal, Roshni Pillai ... Sonia Q Sen
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Computational and Systems Biology

    Subtle methodological variations substantially impact correlation test results in ecological time series

    Caroline Cannistra, Linh Hoang ... Wenying Shou
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Ecology
    2. Evolutionary Biology

    Kinematics and morphological correlates of descent strategies in arboreal mammals suggest early upright postures in euprimates

    Séverine LD Toussaint, Dionisios Youlatos, John A Nyakatura
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Computational and Systems Biology

    Twist is the key to the gating of mechanosensitive ion channel NOMPC

    Jingze Duan, Chen Song
    Rotation of the transient receptor potential (TRP) domain appears to be a universal mechanism for gating of the TRP channel family, including the mechanosensitive ion channel NOMPC.
    1. Neuroscience

    3D directional tuning in the orofacial sensorimotor cortex during natural feeding and drinking

    Victoria B Hosack, Fritzie Arce-McShane
    There is significant information about continuous 3D tongue direction contained in the primate orofacial sensorimotor cortex during untrained tasks, and alterations to tongue kinematics and directional tuning with temporarily induced sensory loss.
    1. Neuroscience

    Asymmetric cortical projections to striatal direct and indirect pathways distinctly control actions

    Jason R Klug, Xunyi Yan ... Xin Jin
    A modified rabies-based circuit-mapping strategy combined with physiology, optogenetics, and behavior identifies asymmetric corticostriatal subcircuits that exert distinct control over actions.
    1. Neuroscience

    Exploring neurodevelopment via spatiotemporal collation of anatomical networks with NeuroSC

    Noelle L Koonce, Sarah E Emerson ... Daniel A Colón-Ramos
    NeuroSC provides an open-source framework for comparative connectomics, integrating neuronal morphologies, contacts, and synapses across developmental stages and datasets.
    1. Cancer Biology

    TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer

    Laura Morano, Nadia Vezzio-Vié ... Jihane Basbous
    Pharmacological disruption of TopBP1 condensation impairs ATR signaling, promotes DNA damage and apoptosis, and enhances the efficacy of FOLFIRI chemotherapy in colorectal cancer.
    1. Microbiology and Infectious Disease

    The capsule and genetic background, rather than specific individual loci, strongly influence in vitro pneumococcal growth kinetics

    Chrispin Chaguza, Daan W Arends ... Amelieke JH Cremers
    The pneumococcal capsular serotype and genetic background, as well as a combination of genomic loci, influence intrinsic pneumococcal growth kinetics, which may have implications for pneumococcal disease pathogenesis.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Decoding molecular mechanisms for loss-of-function variants in the human proteome

    Matteo Cagiada, Nicolas Jonsson, Kresten Lindorff-Larsen
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    • Solid
    1. Structural Biology and Molecular Biophysics

    Protein language model identifies disordered, conserved motifs implicated in phase separation

    Yumeng Zhang, Jared Zheng, Bin Zhang
    Conserved sequence motifs within intrinsically disordered protein regions act as evolutionary units that support phase separation and membraneless organelle formation.
    1. Cancer Biology

    Erythrocytosis-inducing PHD2 mutations implicate biological role for N-terminal prolyl-hydroxylation in HIF1α oxygen-dependent degradation domain

    Cassandra C Taber, Wenguang He ... Michael Ohh
    A unique pathogenic mutation on the primary prolyl hydroxylase of the oxygen-sensing pathway compromises the capacity to hydroxylate the hypoxia-inducible factor's N-terminal oxygen-dependent degradation domain proline while retaining activity against the otherwise catalytically predominant C-terminal proline.
    1. Immunology and Inflammation

    Resident memory macrophages and trained innate immunity at barrier tissues

    Alisha Kang, Michael D'Agostino ... Zhou Xing
    The increasing knowledge of barrier tissue-resident memory macrophages and trained innate immunity (TII) will help develop both nontarget-specific and target-specific TII-based vaccine strategies.
    1. Neuroscience

    The unique synaptic circuitry of specialized olfactory glomeruli in Drosophila melanogaster

    Lydia Gruber, Rafael Cantera ... Jürgen Rybak
    A novel connectomics approach reveals synaptic connectivity differences between narrowly and broadly tuned olfactory glomeruli in Drosophila melanogaster, providing new insights into glomerular circuitry and its putative computational roles.
    1. Neuroscience

    Probing the role of synaptic adhesion molecule RTN4RL2 in setting up cochlear connectivity

    Nare Karagulyan, Maja Überegger ... Christine Bandtlow
    Loss of RTN4RL2 disrupts synapse formation and function between inner hair cells and spiral ganglion neurons, leading to elevated auditory thresholds and highlighting its critical role in hearing.
    1. Immunology and Inflammation

    Lipopolysaccharide stimulates dynamic changes in B cell metabolism to promote proliferation

    Dana MS Cheung, Momchil Razsolkov ... J Simon C Arthur
    Not revised
    Reviewed Preprint v1
    • Fundamental
    • Convincing
    1. Medicine

    Mid-zone hepatocytes trade proliferation for survival via Atf4-Chop axis in early acute liver injury

    Yaying Zhu, Chengxiang Deng ... Zhao Shan
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Cancer Biology

    Blocking Osteoprotegerin Reprograms Cancer Associated Fibroblast to Promotes Immune Infiltration into the Tumor Microenvironment

    Yao Wang, Hara Apostolopoulou ... Anil Bhushan
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Genetics and Genomics

    BreakLoops: A New Feature for the Multi-Gene, Multi-Cancer Family History-Based Model, Fam3Pro

    Nicolas Kubista, Ryan Hernandez-Cancela ... Danielle Braun
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Neuroscience

    Remote automated delivery of mechanical stimuli coupled to brain recordings in behaving mice

    Justin Burdge, Anissa Jhumka ... Ishmail Abdus-Saboor
    Development of robotic device for pain and somatosensory assays in rodents allows remote, quantitative, and reproducible delivery of mechanical stimuli.
    1. Genetics and Genomics

    Alternative splicing across the tree of life

    Rebeca de la Fuente, Wladimiro Dı́az-Villanueva ... Andres Moya
    Alternative splicing exhibits lineage-specific variability, with mammals and birds reaching the highest levels despite conserved intron-rich architectures, while unicellular eukaryotes and prokaryotes display only minimal values.
    1. Immunology and Inflammation

    scRNA+TCR-seq reveals the proportion and characteristics of dual TCR Treg cells in mouse lymphoid and non-lymphoid tissues

    Yuanyuan Xu, Qi Peng ... Xinsheng Yao
    scRNA+TCR-seq technology identifies a high proportion of dual TCR Treg cells in both lymphoid and non-lymphoid tissues of mice, revealing their tissue specificity, TCR repertoire characteristics, and functional phenotypes.
    Version of Record
    Short Report
    • Useful
    • Incomplete
    1. Neuroscience

    Adult neurogenesis reconciles flexibility and stability of olfactory perceptual memory

    Bennet Sakelaris, Hermann Riecke
    Transient intrinsic properties of young neurons enable fast learning and support memory stability as the neurons mature, offering a coherent view of the function of adult neurogenesis in olfaction.
    1. Immunology and Inflammation

    Interleukin 10 controls the balance between tolerance, pathogen elimination, and immunopathology in birds

    Dominique Meunier, Ricardo Corona-Torres ... David A Hume
    The impact of IL10 mutation in birds highlights the intrinsic compromise between effective elimination of a pathogen and damage to the host.
    1. Cell Biology
    2. Developmental Biology

    Cross-species insemination reveals mouse sperm ability to enter and cross the fish micropyle

    Suma Garibova, Eva Stickler ... Matteo Avella
    Mouse sperm are unable to bind the zebrafish chorion, yet a subset of hyperactivated sperm can enter and cross the micropyle, suggesting a conserved mechanism of gamete interaction across species.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    A recursive pathway for isoleucine biosynthesis arises from enzyme promiscuity

    Vittorio Rainaldi, Stefano Donati ... Nico J Claassens
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Structural Biology and Molecular Biophysics
    2. Microbiology and Infectious Disease

    Evolution of a fuzzy ribonucleoprotein complex in viral assembly

    Huaying Zhao, Tiansheng Li ... Peter Schuck
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Cancer Biology
    2. Computational and Systems Biology

    BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment

    Emilio Rios-Jimenez, Anoek Zomer ... Maria Alieva
    BEHAV3D Tumor Profiler enables researchers without computational expertise to uncover heterogeneous cell behaviors and explore their relationships with microenvironmental features in intravital microscopy data.
    1. Neuroscience

    Toward Robust Neuroanatomical Normative Models: Influence of Sample Size and Covariates Distributions

    Camille Elleaume, Bruno Hebling Vieira ... the Australian Imaging Biomarkers and Lifestyle flagship study of ageing
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Adult-neurogenesis allows for representational stability and flexibility in early olfactory system

    Zhen Chen, Krishnan Padmanabhan
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Homeostatic synaptic plasticity of miniature excitatory postsynaptic currents in mouse cortical cultures requires neuronal Rab3a

    Andrew G Koesters, Mark M Rich, Kathrin Engisch
    The first demonstration that a protein thought to function presynaptically (Rab3a) is required for homeostatic, activity-dependent synaptic plasticity of quantal size in central neurons.
    1. Chromosomes and Gene Expression
    2. Developmental Biology

    HIF1A-mediated pathways promote euploid cell survival in chromosomally mosaic embryos

    Estefania Sanchez-Vasquez, Marianne E Bronner, Magdalena Zernicka-Goetz
    Aneuploid mosaic mouse embryos require hypoxia for survival, and understanding these pathways may inform new strategies to support embryo viability.
    1. Neuroscience
    2. Computational and Systems Biology

    Absence of Systematic Effects of Internalizing Psychopathology on Learning Under Uncertainty

    Muhammad H Satti, Katharina Wille ... Rasmus Bruckner
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Cell Biology
    2. Neuroscience

    Death receptor 6 does not regulate axon degeneration and Schwann cell injury responses during Wallerian degeneration

    Bogdan Beirowski, Haoran Huang, Elisabetta Babetto
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Convincing
    1. Physics of Living Systems

    The Matthew effect and early-career setbacks in research funding—a replication study

    Vincent Traag, Emer Brady ... Carter Bloch
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Frequency and Laminar Profile of Feature Specific Visual Activity Revealed by Interleaved EEG-fMRI

    Tommy Clausner, José P Marques ... Mathilde Bonnefond
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Explainable machine learning-assisted exploration of chromatin dynamics reveals chromosome-specific response to serum starvation

    Taras Redchuk, Antti Pennanen ... Maria K Vartiainen
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    1. Neuroscience

    Distinct cortical encoding of acoustic and electrical cochlear stimulation

    Ariel Edward Hight, Michele N Insanally ... Robert C Froemke
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Allocentric and egocentric cues constitute an internal reference frame for real-world visual search

    Yan Chen, Zhe-Xin Xu
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Structural Biology and Molecular Biophysics

    Active regulation of the epidermal growth factor receptor by the membrane bilayer

    Shwetha Srinivasan, Xingcheng Lin ... Gabriela S Schlau-Cohen
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology

    Overexpression of Ssd1 and calorie restriction extend yeast replicative lifespan by preventing deleterious age-dependent iron uptake

    J Ignacio Gutierrez, Claudia Edgar, Jessica K Tyler
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology

    Organelle membrane-associated proteins recruit cGAS via phase separation to facilitate its membrane localization

    Chengrui Shi, Chaofei Su ... Hang Yin
    Not revised
    Reviewed Preprint v1
    • Useful
    • Solid
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Dietary sulfur amino acid restriction elicits a cold-like transcriptional response in inguinal but not epididymal white adipose tissue of male mice

    Philip MM Ruppert, Aylin S Güller ... Jan-Wilhelm Kornfeld
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    Transcranial direct current stimulation modulates primate brain dynamics across states of consciousness

    Guylaine Hoffner, Pablo Castro ... Béchir Jarraya
    The effects of transcranial direct current stimulation on cortical dynamics depend on the state of consciousness and the stimulation polarity.
    1. Neuroscience

    Synchronous ensembles of hippocampal CA1 pyramidal neurons during novel exploration

    En-Li Chen, Tsai-Wen Chen ... Bei-Jung Lin
    CA1 pyramidal neurons exhibit synchronous spiking during movement and pauses in novel environments, reflecting a neural signature of spatial learning.
    1. Neuroscience

    Shared functional organization between pulvinar-cortical and cortico-cortical connectivity and its structural and molecular imaging correlates

    Gianpaolo Antonio Basile, Augusto Ielo ... Alberto Cacciola
    The pulvinar complex harbors multiple representations of cortico-cortical connectivity organized hierarchically across its ventro-dorsal and medio-lateral axes.
    1. Neuroscience

    Age and learning shapes sound representations in auditory cortex during adolescence

    Benedikt Praegel, Feng Chen ... Adi Mizrahi
    Weaker performance in adolescent mice arises from immature cortical representations and cognitive bias, linking developmental changes in behavior with underlying neural coding.
    1. Cancer Biology

    KDM5 demethylases suppress R-loop-mediated ‘viral mimicry’ and DNA damage in breast cancer cells

    Lena Lau, Kurt Henderson ... Marie Classon
    KDM5 inhibition/disruption selectively induces R-loop-mediated DNA damage and innate immune activation in breast cancer cells, while sparing normal cells, unveiling a tumor-specific therapeutic vulnerability with broad translational potential.
    1. Neuroscience

    Backward Conditioning Reveals Flexibility in Infralimbic Cortex Inhibitory Memories

    Nura W Lingawi, Billy C Chieng ... Vincent Laurent
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    • Incomplete
    1. Neuroscience

    Enhancer-AAVs allow genetic access to oligodendrocytes and diverse populations of astrocytes across species

    John K Mich, Smrithi Sunil ... Boaz P Levi
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Structural Biology and Molecular Biophysics

    Dynamic Architecture of Mycobacterial Outer Membranes Revealed by All-Atom Simulations

    Turner P Brown, Matthieu Chavent, Wonpil Im
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    1. Epidemiology and Global Health

    Forecasting the spatial spread of an Ebola epidemic in real time: Comparing predictions of mathematical models and experts

    James D Munday, Alicia Rosello ... Sebastian Funk
    Models were more consistent than individual experts but performed comparably to ensembles of many experts and relative performance varied by month, justifying the use of combined model and expert forecast methodologies.
    1. Immunology and Inflammation

    Metabolic regulation of immune memory and function of microglia

    Nikolaos Nirakis, Sofia Dimothyra ... Vasileia Ismini Alexaki
    Cell metabolic processes, particularly glucose, glutamine, and lipid metabolism, orchestrate inflammatory responses and promote epigenetic changes shaping microglia immune memory.
    1. Cell Biology
    2. Developmental Biology

    Wnt induces FZD5/8 endocytosis and degradation and the involvement of RSPO-ZNRF3/RNF43 and DVL

    Dong Luo, Jing Zheng ... Xinjun Zhang
    RSPO-ZNRF3/RNF43 specifically regulates the degradation of FZD5/8 under Wnt stimulation independently of DVL.
    1. Cancer Biology
    2. Evolutionary Biology

    Cancer–immune coevolution dictated by antigenic mutation accumulation

    Long Wang, Christo Morison, Weini Huang
    Genetic information in cancer cells reveals the strength of cancer–immune interactions in an individual-based stochastic model, which captures explicit interactions between cancer and effector cells.
    1. Neuroscience

    When word order matters: human brains represent sentence meaning differently from large language models

    James Fodor, Carsten Murawski, Shinsuke Suzuki
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Immunology and Inflammation
    2. Evolutionary Biology

    Evolutionary Adaptations of IRG1 Refines Itaconate Synthesis and Mitigates Innate Immunometabolism Trade-offs

    Richard V Szeligowski, Francois Miros ... Hongying Shen
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Functional connectivity, structural connectivity, and inter-individual variability in Drosophila melanogaster

    Takuto Okuno, Alexander Woodward ... Junichi Hata
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    Generation of knock-in Cre and FlpO mouse lines for precise targeting of striatal projection neurons and dopaminergic neurons

    Eddy Albarran, Akira Fushiki ... Rui M Costa
    Not revised
    Reviewed Preprint v1
    • Important
    • Compelling
    • Incomplete
    1. Microbiology and Infectious Disease

    Heterogeneity of Genetic Sequence within Quasi-species of Influenza Virus Revealed by Single-Molecule Sequencing

    Kenji Tamao, Hiroyuki Noji, Kazuhito V Tabata
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Computational and Systems Biology

    TrueProbes: Quantitative Single-Molecule RNA-FISH Probe Design Improves RNA Detection

    Jason J Hughes, Benjamin K Kesler ... Gregor Neuert
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    Chronic stress impairs autoinhibition in neurons of the locus coeruleus to increase asparagine endopeptidase activity

    Hiroki Toyoda, Doyun Kim ... Youngnam Kang
    Chronic stress impairs the autoinhibition in locus coeruleus neurons by inducing the internalization of α2A adrenaline receptors, which causes their overexcitation and consequently induces their degeneration by producing active asparagine endopeptidase.
    1. Ecology

    Desmodium volatiles in ‘push-pull’ cropping systems and protection against the fall armyworm, Spodoptera frugiperda

    Daria M Odermatt, Frank Chidawanyika ... Meredith C Schuman
    'Push-pull' agro-ecosystems appear to protect maize against the fall armyworm, but volatiles from Desmodium intercrops are not sufficient to reliably repel this herbivore, indicating that additional mechanisms contribute to protection.
    1. Epidemiology and Global Health

    Reassessing the link between adiposity and head and neck cancer: a Mendelian randomization study

    Fernanda Morales Berstein, Jasmine Khouja ... Rebecca C Richmond
    Adiposity does not play a major role in head and neck cancer risk.
    1. Ecology

    Partitioning changes in ecosystem productivity by effects of species interactions in biodiversity experiments

    Jing Tao, Charles A Nock ... Ji Zheng
    A novel approach has been developed to partition the net biodiversity effect into components associated with positive, negative, and competitive species interactions.
    1. Neuroscience

    Diffusion MRS tracks distinct trajectories of neuronal development in the cerebellum and thalamus of rat neonates

    Clémence Ligneul, Lily Qiu ... Jason P Lerch
    Diffusion-weighted magnetic resonance spectroscopy and microstructural modelling longitudinally tracks cell-specific cerebellar development to non-invasively monitor, and better understand, early brain microstructure.
    1. Developmental Biology
    2. Neuroscience

    Premature vision drives aberrant development of response properties in primary visual cortex

    Sophie V Griswold, Stephen D Van Hooser
    Introducing premature visual experience by opening the closed eyelids early in ferrets caused many cells in the primary visual cortex to mature improperly.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    High-content high-resolution microscopy and deep learning-assisted analysis reveals host and bacterial heterogeneity during Shigella infection

    Ana Teresa López-Jiménez, Dominik Brokatzky ... Serge Mostowy
    High-content high-resolution microscopy coupled with deep learning-based analysis reveals novel phenotypes at the single-cell level during Shigella infection.
    1. Cell Biology

    Megakaryocytes assemble a three-dimensional cage of extracellular matrix that controls their maturation and anchoring to the vascular niche

    Claire Masson, Cyril Scandola ... Anita Eckly
    Megakaryocytes form a 3D cage of laminin and collagen IV that stabilizes them within their vascular niche, while β1/β3 integrins and MMP are key regulators that assist megakaryocyte maturation and intravasation.
    1. Neuroscience

    Bayesian meta-analysis reveals the mechanistic role of slow oscillation-spindle coupling in sleep-dependent memory consolidation

    Thea Ng, Eunsol Noh, Rebecca MC Spencer
    Strong and temporally precise coupling between frontal slow oscillations and fast spindles consistently predicts memory retention across conditions, supporting its role as a fundamental mechanism in sleep-dependent memory consolidation.
    1. Cell Biology
    2. Medicine

    Uncovering Shared and Tissue-Specific Molecular Adaptations to Intermittent Fasting in Liver, Brain, and Muscle

    Yibo Fan, Senuri De Silva ... Thiruma V Arumugam
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Biochemistry and Chemical Biology

    Stall force measurement of the kinesin-3 motor KIF1A using a programmable DNA origami nanospring

    Nobumichi Takamatsu, Hiroko Furumoto ... Kumiko Hayashi
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Social experience shapes fighting strategies in Drosophila

    Can Gao, Mingze Ma ... Yufeng Pan
    Social enrichment increases the less-frequent high-intensity aggressive tussling and decreases the low-intensity aggressive lunging via distinct sensory and central neurons.
    1. Neuroscience

    Distributed subthreshold representation of sharp wave-ripples by hilar mossy cells

    Ayako Ouchi, Taro Toyoizumi ... Yuji Ikegaya
    Machine learning with whole-cell patch-clamp and local field potential recordings reveals that synaptic responses from five mossy cells, despite their limited population, can significantly reconstruct about 30% of the total hippocampal sharp wave-ripples, highlighting efficient distributed coding within subthreshold activities.
    1. Plant Biology

    Structure-guided secretome analysis of gall-forming microbes offers insights into effector diversity and evolution

    Soham Mukhopadhyay, Muhammad Asim Javed ... Edel Perez-Lopez
    Comparative structural genomics identifies unique effector architectures and ankyrin-mediated host targeting, revealing central and conserved strategies that underpin the pathogenicity of diverse gall-forming microbial species across evolutionary lineages.
    1. Neuroscience

    A unified rodent atlas reveals the cellular complexity and evolutionary divergence of the dorsal vagal complex

    Cecilia Hes, Abigail J Tomlinson ... Paul V Sabatini
    The dorsal vagal complex is a highly intricate structure composed of distinct transcriptional classes of glial and neuronal cells, exhibiting many similarities but also notable differences between mice and rats.
    1. Structural Biology and Molecular Biophysics

    DIRseq as a method for predicting drug-interacting residues of intrinsically disordered proteins from sequences

    Matt MacAinsh, Sanbo Qin, Huan-Xiang Zhou
    A sequence-based method, DIRseq, has been developed to predict drug-interacting residues of intrinsically disordered proteins.
    1. Physics of Living Systems

    Balancing stability and flexibility when reshaping archaeal membranes

    Miguel Amaral, Felix Frey ... Anđela Šarić
    Archaeal cells mix single-headed and double-headed lipids to construct resilient but flexible membranes to help them survive in extreme environments.
    1. Developmental Biology

    Distinct waves of ovarian follicles contribute to mouse oocyte production

    Qi Yin, Allan C Spradling
    The first follicle wave undergoes atresia to amplify androgen-producing theca cells, while previously unknown wave 1.5 follicles leave quiescence at 2 weeks and produce most of the earliest offspring.
    1. Cell Biology

    The Multifaceted Role of EXOC6A in Ciliogenesis

    Te-Lin Lin, Chien-Ting Wu, Tang K Tang
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Two classes of amine/glutamate multi-transmitter neurons innervate Drosophila internal male reproductive organs

    Martha Chaverra, John Paul Toney ... R Steven Stowers
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Microbiology and Infectious Disease

    ZFT is the major iron and zinc transporter in Toxoplasma gondii

    Dana Aghabi, Cecilia Gallego Rubio ... Clare R Harding
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Heterochronic transcription factor expression drives cone-dominant retina development in 13-lined ground squirrels

    Kurt Weir, Pin Lyu ... Seth Blackshaw
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    • Incomplete
    1. Neuroscience

    Adaptive behavior is guided by integrated representations of controlled and non-controlled information

    Bingfang Huang, Harrison Ritz, Jiefeng Jiang
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Cell Biology

    Thermogenic Adipose ADH5 Counteracts Age-related Metabolic Decline

    Sara C Sebag, Tate Neff ... Ling Yang
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience
    2. Computational and Systems Biology

    Deep Neural Networks to Register and Annotate Cells in Moving and Deforming Nervous Systems

    Adam A Atanas, Alicia Kun-Yang Lu ... Steven W Flavell
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    • Incomplete
    1. Ecology
    2. Epidemiology and Global Health

    Rift Valley fever virus dynamics in a transhumant cattle system in The Gambia

    Essa Jarra, Divine Ekwem ... Daniel T Haydon
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    Oxytocin neurons signal state-dependent transitions from rest to thermogenesis and behavioral arousal in social and non-social settings

    Morgane Vandendoren, Jason G Landen ... Adam C Nelson
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Developmental Biology
    2. Neuroscience

    Neuronal detection triggers systemic digestive shutdown in response to adverse food sources in Caenorhabditis elegans

    Yating Liu, Guojing Tian ... Bin Qi
    A neural-digestive mechanism has been presented for evaluating harmful food.
    1. Neuroscience

    Optogenetic stimulation of single ganglion cells in the living primate fovea

    Peter J Murphy, Juliette E McGregor ... David R Williams
    Individual retinal ganglion cells are singularly activated in the living primate retina via an adaptive optics scanning light ophthalmoscope.
    1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    Pan-tissue transcriptome analysis reveals sex-dimorphic human aging

    Siqi Wang, Danyue Dong ... Zefeng Wang
    Widespread sex-dimorphic transcriptomic changes during human aging in pan-tissues scale reveal distinct molecular aging trajectories and potential links to age-related disease vulnerability.
    1. Neuroscience

    Dual transcranial electromagnetic stimulation of the precuneus boosts human long-term memory

    Ilaria Borghi, Lucia Mencarelli ... Giacomo Koch
    Rapid, combined and personalized non-invasive stimulation of the precuneus improves associative memory, enhances local gamma expression, and strengthens precuneus–hippocampal connectivity.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Pleomorphic effects of three small-molecule inhibitors on transcription elongation by Mycobacterium tuberculosis RNA polymerase

    Omar Herrera-Asmat, Alexander B Tong ... Carlos Bustamante
    Optical tweezers are used to gain mechanistic insight in the mechanism of inhibition of three distinct compounds being developed as antibiotics against Mycobacterium tuberculosis.
    1. Cancer Biology
    2. Evolutionary Biology

    Punctuated mutagenesis promotes multi-step evolutionary adaptation in human cancers

    Christopher Graser, Wenbo Wu ... Franziska Michor
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Squidly: Enzyme Catalytic Residue Prediction Harnessing a Biology-Informed Contrastive Learning Framework

    William JF Rieger, Mikael Boden ... Ariane Mora
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience
    2. Computational and Systems Biology

    Comparing the outputs of intramural and extramural grants funded by National Institutes of Health

    Xiang Zheng, Qiyao Yang ... B Ian Hutchins
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Neuroscience

    Fitting bifurcation structure, not voltage traces: A biophysically inspired derivation of reduced neuron models exemplified by potassium dynamics

    Louisiane Lemaire, Mahraz Behbood ... Susanne Schreiber
    Not revised
    Reviewed Preprint v1
    • Useful
    • Convincing
    1. Immunology and Inflammation

    A co-evolutionary perspective on humans and Mycobacterium tuberculosis in the era of systems biology

    Michaela Reichmann, Liku B Tezera ... Paul Elkington
    Not revised
    Reviewed Preprint v1
    1. Epidemiology and Global Health
    2. Computational and Systems Biology

    Refining uncertainty about the TAK-003 dengue vaccine with a multi-level model of clinical efficacy trial data

    Manar Alkuzweny, Guido España, T Alex Perkins
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    Two time scales of adaptation in human learning rates

    Jonas Simoens, Senne Braem ... Tom Verguts
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Neuroscience

    Age-related changes in ‘cortical’ 1/f dynamics are linked to cardiac activity

    Fabian Schmidt, Sarah K Danböck ... Nathan Weisz
    Age-related differences in cortical aperiodic signals can be driven by cardiac influences, emphasizing the need to control for cardiac contributions in studies interpreting cortically measured aperiodic dynamics.
    1. Neuroscience

    Balancing safety and efficiency in human decision-making

    Pranav Mahajan, Shuangyi Tong ... Ben Seymour
    A separate fear system, flexibly gated by outcome uncertainty, guides decision-making to be both safe and efficient during exploration.
    1. Cell Biology
    2. Neuroscience

    Endophilin A1 facilitates organization of the GABAergic postsynaptic machinery to maintain excitation-inhibition balance

    Xue Chen, Deng Pan ... Yanrui Yang
    Endophilin A1 at postsynaptic sites promotes inhibitory postsynaptic organization through interaction with gephyrin to maintain E/I balance, which is essential for the suppression of epilepsy susceptibility.
    1. Immunology and Inflammation

    Trained immunity in skin infections: Macrophages and beyond

    Vitka Gres, Merve Göcer ... Philipp Henneke
    Review of the emerging understanding of trained immunity in the skin and how infection-driven cellular processes induce long-lasting immune adaptation and modulate skin barrier integrity.
    1. Chromosomes and Gene Expression

    Genome organization by SATB1 binding to base-unpairing regions (BURs) provides a scaffold for SATB1-regulated gene expression

    Yoshinori Kohwi, Xianrong Wong ... Terumi Kohwi-Shigematsu
    Modified ChIP-seq and 4C-seq approaches, using urea-purified crosslinked chromatin, uncovered a two-tiered chromatin organization mediated by SATB1 that is linked to gene expression.
    1. Epidemiology and Global Health
    2. Genetics and Genomics

    Understanding the global rise of artemisinin resistance: Insights from over 100,000 Plasmodium falciparum samples

    Andrew J Balmer, Nina FD White ... Cristina Ariani
    Spatiotemporal analysis of global kelch13 surveillance data reveals that artemisinin resistance is increasing in East Africa, mirroring patterns previously observed in Southeast Asia.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Lipid peroxidation and type I interferon coupling fuels pathogenic macrophage activation causing tuberculosis susceptibility

    Shivraj M Yabaji, Vadim Zhernovkov ... Igor Kramnik
    Myc dysregulation in activated macrophages impairs antioxidant defenses, locking them in a vicious cycle that amplifies type I interferon signaling, driving hyperinflammation and immunosuppression.
    1. Microbiology and Infectious Disease
    2. Epidemiology and Global Health

    The age and sex dynamics of heterosexual HIV transmission in Zambia: an HPTN 071 (PopART) phylogenetic and modelling study

    Matthew D Hall, William Probert ... the PANGEA-HIV consortium and the HPTN 071 (PopART) study team
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Orco regulates the circadian activity of pheromone-sensitive olfactory receptor neurons in hawkmoths

    Aditi Vijayan, Mauro Forlino ... Monika Stengl
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Neuroscience

    Neural Representation of Associative Threat Learning in Pulvinar Divisions, Lateral Geniculate Nucleus, and Mediodorsal Thalamus in Humans

    Muhammad Badarnee, Zhenfu Wen ... Mohammed R Milad
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Incomplete
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Tracking maternal proteins uncovers a central role for the residual body in organelle recycling during Toxoplasma gondii replication

    Julia von Knoerzer-Suckow, Parnian Sazegar ... Markus Meissner
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    • Incomplete
    1. Microbiology and Infectious Disease
    2. Ecology

    BuzzWatch: Uncovering Multi-scale Temporal Patterns in Mosquito Behavior Through Continuous Long-term Monitoring

    Théo Maire, Zhong Wan ... Felix JH Hol
    Not revised
    Reviewed Preprint v1
    • Useful
    • Incomplete
    1. Neuroscience

    Cortical projection neurons with distinct axonal connectivity employ ribosomal complexes with distinct protein compositions

    Tien Phuoc Tran, Bogdan Budnik ... Jeffrey D Macklis
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Compelling
    1. Neuroscience

    Micro-scale control of oligodendrocyte morphology and myelination by the intellectual disability-linked protein acyltransferase ZDHHC9

    Hey-Kyeong Jeong, Estibaliz Gonzalez-Fernandez ... Gareth M Thomas
    Loss of ZDHHC9, a protein acyltransferase that is highly expressed in oligodendrocytes, does not grossly impact myelination but markedly alters oligodendrocyte morphology and myelination at the micro/nano-scale.
    1. Neuroscience

    Patchy striatonigral neurons modulate locomotor vigor in response to environmental valence

    Sarah Hawes, Bo Liang ... Huaibin Cai
    Patchy striatonigral neurons regulate implicit locomotor speed selection based on environmental valence, revealing a subtype-specific mechanism by which striatal circuits shape adaptive motor control and value-dependent behavioral responses.
    1. Neuroscience

    The influence of temporal context on vision over multiple time scales

    Kacie Lee, Reuben Rideaux
    Across multiple scales, temporal context shapes visual perception through an attention-dependent process facilitating rapid motor responses and an attention-independent process suppressing neural encoding of expected events.

Magazine

    1. Cell Biology
    2. Chromosomes and Gene Expression

    Chromosomes: Exploring a crossroads in the aging process

    Kiyan Shabestary, Daniel E Gottschling
    1. Neuroscience

    Neuronal Migration: How hunger guides new brain cells to their destination

    Fang-Shin Nian, Laurent Nguyen
    1. Neuroscience

    Primate Research: Probing the staying power of chemogenetics

    Maria Puchik, Igor Kagan
    1. Genetics and Genomics
    2. Neuroscience

    Aging: Unlocking the longevity code with stress

    Loren Cocciolone, Valeria Uvarova, Patricija van Oosten-Hawle
    1. Developmental Biology

    Blastomeres: Tracing cell fates in embryos

    Ying Zhang, Qi Chen