An on-demand seizure model in chronically epileptic mice integrates reliability and etiological relevance, providing a mechanistically grounded, efficient platform for evaluating pharmacological interventions.
Movie alignment software uses a 3D spline model to capture beam-induced motion in cryo-electron microscopy movie frames, correct local sample distortions, and improve two-dimensional template matching signal-to-noise ratios in the corrected images.
During convergent extension morphogenesis, non-canonical Wnt induces the signal transducer Dishevelled to transition from Vangl to Frizzled, a process that is inhibited by Prickle but facilitated by the co-receptor Ror.
Resolving the mechanism of RXR ligand-dependent NR4A-RXR nuclear receptor heterodimer activation requires a diverse ligand set that includes heterodimer-selective agonists.
Chengcheng Fan, Alexander A Cohen ... Magnus AG Hoffmann
Bivalent EABR mRNA boosters broaden neutralizing responses to Omicron subvariants by promoting diverse receptor-binding domain epitope targeting, although antibody responses remain partially shaped by immune imprinting from prior vaccination.
Emerson M Carmona, William N Zagotta, Sharona E Gordon
A fluorescent noncanonical amino acid enables structural measurements across full-length human voltage-gated proton channel (Hv1) and reveals zinc-dependent conformational changes.
Computer simulations reveal the structural basis for a selective mechanism of ion-channel inhibition that could guide future pharmacological developments against associated human health disorders.
The thermosensory neuron AFD unexpectedly modulates locomotion based on tactile experience through molecular and circuit mechanisms distinct from those underlying thermosensation.
Genetic experiments combined with cellular and biochemical analyses including lipidomics performed by imaging mass spectrometry reveal that the capacity of mature B lymphocytes and germinal center B cells to synthesize some ether phospholipids is vital for normal humoral responses.
Single-cell and bulk lineage tracing reveals that adult bone marrow endothelial cells generate functional hematopoietic progenitor and mature blood cells, expanding the cell sources of postnatal hematopoiesis.
The kinocilium of vestibular hair cells is a unique organelle with molecular features of primary and motile cilia and may serve as an active, force-generating element within the hair bundle.
Behavioral analyses combined with genetics revealed that Caenorhabditis elegans uses an olfactory receptor-ligand module to forage for essential nutrients.
Harikrishnan Rajendran, Roi Weinberger ... Ofer Feinerman
The architecture of an ant nest serves as a fossilized record of the colony's demographic history, with distinct growth patterns and catastrophic events through age-specific digging behaviors.
A scalable zebrafish imaging platform reveals that Foxp1 paralogues have non-redundant roles in developmental patterning of adipose morphology and adaptive remodelling capacity in response to a high-fat diet.
Endophilin-A3-mediated clathrin-independent endocytosis and retrograde trafficking in cancer cells regulate ICAM1 organization at the immune synapse and influence cytotoxic CD8 T cell response.
Andrés Pablo Varani, Caroline Mailhes-Hamon ... Daniela Popa
Distinct cerebellar projections to the forebrain differentially support acquisition and offline consolidation of a motor skill engaging cerebello-striato-cortical circuits, revealing the temporal and functional diversity of cerebellar contributions to learning.
Colin Bredenberg, Fabrice Normandin ... Guillaume Lajoie
Computational simulations indicate that classical psychedelics could induce hallucinations by co-opting neural circuitry dedicated to sleep-dependent replay and consolidation, providing a theoretical link between seemingly disparate fields of inquiry.
Rats with a history of cocaine use exhibited prolonged encoding of idiosyncratic task features in orbitofrontal cortex and a reduced ability to compress such features to identify underlying hidden states.
A velocity-nulled 3T GE-EPI fMRI method enabling 0.9-mm whole-brain imaging that suppresses vascular contamination and reliably maps layer-specific functional connectivity in human cortex.
Victoria Mischley, Johannes Maier ... John Karanicolas
PPIscreenML is a rigorously benchmarked method that uses AlphaFold2 to screen for interacting protein pairs, and it provides superior performance to other available methods.
Interactions between pairs of humans lead to the emergence of a dyadic vigor with similar characteristics as individual vigor, where both partners adapt, with the slower having a critical role.
Graph theory and computational modeling reveal that neural network architecture biases the male Caenorhabditis elegans brain toward prioritized sexual behaviors.
High‑resolution cryo‑EM structures uncover YbjP as a novel lipoprotein that positions TolC and supports conformational changes in E. coli drug efflux pumps mediating antibiotic resistance.
New mouse genetic tools, roxCre and loxCre, facilitate the study of gene function in cells of interest, allowing for simultaneous and efficient gene knockout and precise lineage tracing in vivo.
Modality-agnostic decoders leverage modality-invariant representations in human subjects' brain activity to predict stimuli irrespective of their modality (image, text, mental imagery).
Mammalian cells dynamically scale translation initiation to match distinct elongation rates, preventing ribosome crowding and preserving protein synthesis homeostasis across diverse transcripts.
Yuri Z Sinzato, Robert Uittenbogaard ... Maziyar Jalaal
Flow experiments show how the formation of cyanobacterial colonies is driven by cell division, aggregation, and fragmentation under different hydrodynamic conditions.
Meet K Patel, Warlen Pereira Piedade, Jakub K Famulski
Zebrafish cone photoreceptor outer segment integrity may be regulated by the interactions between the outer segments and calyceal processes mediated by retinal cadherins cdhr1a and pcdh15b.
Zhimin Lao, Salsabiel El Nagar ... Alexandra L Joyner
Homozygous but not heterozygous loss of Pten in sporadic mouse models of SHH-medulloblastoma greatly accelerates tumor formation, not metastasis, through increased survival of differentiated cells and possibly decreased infiltrating macrophages.
Systematic ChIP-seq profiling of 172 transcription factors in Pseudomonas aeruginosa reveals a hierarchical regulatory architecture governing virulence and establishes a searchable database to guide antimicrobial drug discovery.
Structural and biochemical analyses identify a bacterial lipoprotein that associates with the outer membrane component of tripartite multi-drug efflux pumps, and its potential functional roles are explored by complementary bioinformatics, proteomcid and genetic approaches.
Placenta-binding parasites show that chromatin-based mechanisms regulate placental malaria virulence gene, var2csa, and requires loss of H3K9me3-mediated heterochromatic silencing and 3D nuclear repositioning away from telomeric-repressive clusters.
Identification of each animal in a collective becomes possible even when individuals are never all visible simultaneously, enabling faster and more accurate analysis of collective behavior.
A theory of efficient coding wherein the precision of representations is endogenous, task-dependent, and prior-dependent predicts scaling laws for imprecision and is supported by numerosity perception experiments with humans.
Synaptotagmin 1 and Synaptotagmin 7 promote MAIT cell activation during Mycobacterium tuberculosis infection by facilitating MR1 trafficking and antigen presentation.
Ken-ichiro F Kamei, Koseki J Kobayashi-Kirschvink ... Yuichi Wakamoto
Conservation levels of gene expression abundance ratios are globally coordinated in cells, and cellular state changes under such biologically relevant stoichiometric constraints are readable as low-dimensional changes in Raman spectra.
Cargo-adaptor binding to the KLC-TPR domains binding triggers structural rearrangements that relieve kinesin-1 autoinhibition, enabling MAP7 recruitment, through an allosteric mechanism that connects cargo recognition to motor activation.
Geometric confinement paradoxically enhances bacterial chemotaxis through chiral surface swimming and sidewall alignment, with optimal performance when lane width matches the circular swimming radius.
Olfactory fear conditioning biases olfactory stem cell receptor fate, increasing the frequency by which maturing neurons express the receptor of the paired odor and changing the representation of the olfactory sensory neuron landscape in the next generation.
Shwetha Srinivasan, Xingcheng Lin ... Gabriela S Schlau-Cohen
Membrane composition modulates EGFR conformational dynamics and signaling, revealing that the bilayer properties can override ligand control to influence receptor activation in health and disease.
Brett A Hathaway, Dexter R Kim ... Catharine Winstanley
Sensory cues increase risky choice when paired with wins but reduce risky choice when paired with losses, with parallel shifts in sensitivity to negative outcomes.
Maryam Karimian, Mark Jonathan Roberts ... Mario Senden
Principles of weakly coupled oscillators capture human figure-ground segregation and its training-induced enhancement, indicating gamma synchrony remains a plausible grouping mechanism.
Farjana Islam, Cayman Williams ... Benedict Seddon
Inhibitor of κB kinase signalling differentially controls development and survival of γδ T cell subsets through nuclear factor-κB activation and repression of receptor-interacting protein kinase 1-driven necroptosis, revealing distinct inflammatory signalling requirements from αβ T cells.
The ability to predict brain activity from words before they occur can be explained by information shared between neighbouring words, without requiring next-word prediction by the brain.
Genetic and biochemical analyses show that loss of the E3 ubiquitin ligases ZNRF3 and RNF43 enhances EGFR signaling by impairing EGFR ubiquitination and degradation, revealing a new cancer-promoting mechanism.
A system to enrich yeast in metaphase of mitosis, meiosis I, or meiosis II reveals reduced spindle assembly checkpoint robustness in meiosis I and distinct kinetochore composition and phosphorylation states.
The phase of cortical activity, measured in the gray matter, is organized at multiple spatial scales, with the largest scales explaining most variance in phase at a given temporal frequency.
Detecting replay during longer time periods (e.g. resting state or sleep) with temporally delayed linear modeling (TDLM) requires biologically implausible event densities, and purely synthetic simulations substantially overestimate the method's sensitivity.
Direct survival benefits in larger groups rather than kin-selected indirect fitness primarily drive the evolution of division of labor in cooperatively breeding vertebrates, particularly under harsh environmental conditions.
Analysis of scRNA-seq data from primate embryoid bodies highlights the value of cross-species comparisons for identifying and characterizing cell types, as marker genes can evolve fast.
A genetic toolkit establishes Drosophila as a tractable metazoan system for systematic interrogation of conserved biological functions of the ancient polymer polyphosphate.
Elucidation of the Hox code defining forelimb positioning provides novel insights into lateral plate mesoderm patterning and the integration of vertebrate column structure and limb positioning.
Structural characterization of the CTLH-CRA domain-mediated pairing in the CTLH ubiquitin ligase complex enables rational engineering of interfaces with altered binding specificity.
Yuzhen Liu, Christopher D McGann ... Devin K Schweppe
DNA O-MAP enables proximity labeling at specific genomic loci in fixed cells using programmable oligonucleotides, revealing locus-proximal proteomes and chromatin interactions without genetic modification.
Frederick A Matsen IV, Will Dumm ... Hugh K Haddox
Factoring out nucleotide-level mutation biases from antibody language models dramatically improves prediction of functional mutation effects while reducing computational cost by orders of magnitude.
Transcriptomic heterogeneity in infective-stage surface proteins of Trypanosoma cruzi, particularly trans-sialidase-like superfamily members, suggests distinct expression profiles among individual parasites that may enhance immune evasion and infection adaptability.
Genomic neighborhood signatures delineate catalytic versus putative regulatory CODH clades, establishing a framework for predicting enzyme functionality and guiding discovery of biocatalysts for CO2 reduction.
Direct presynaptic patch-clamp recording and fluorescent imaging of the vesicular release demonstrate that a non-canonical cannabinoid receptor GPR55 deprives readily releasable vesicles of competency in cerebellar Purkinje cell axons.
François Simon, Guillaume Ramadier ... Lucien E Weiss
Exatrack is a framework for analyzing Brownian, directed, and confined motion using a hidden-variable probabilistic model to extract physical insight into particle diffusion.
Dysregulated exocytosis of bis(monoacylglycerol)phosphate (BMP)-enriched extracellular vesicles driven by pathogenic LRRK2 activity supports elevated urinary BMP levels and its use as a biomarker for Parkinson's disease.
Peptidoglycan recycling is essential for proper cell growth and intrinsic ampicillin resistance in Caulobacter crescentus, highlighting PG recycling enzymes as potential antibacterial targets.
A hierarchical framework integrating artificial intelligence with chemical principles enables the conversion of fragile α-helices into ultrastable protein scaffolds.
