A large collection of functional EGFP tagged proteins derived from MiMIC insertions allows examination of protein expression in unfixed tissues and efficient tissue specific reversible knock down of proteins.
The discovery of the earliest direct evidence of brood care in insects demonstrates a remarkably conserved egg-brooding reproductive strategy within scale insects in stasis for nearly 100 million years.
The gut-released peptide oxyntomodulin mediates the alignment of liver clock gene and metabolic transcript rhythms with the timing of food intake by postprandial induction of hepatic Per gene expression.
A combination of genetics, expression, genomic localization, and genomic nucleosome profiling demonstrates antagonism between two chromatin remodelers that reveal different mechanisms at different promoter architectures.
Under normal nutritional conditions, G-protein coupled receptors can control autophagy by regulating the degradation of key autophagic regulator Atg14L through ZBTB16-mediated ubiquitination and proteasome degradation.
A computational model for the formation of neural networks of grid cells in virtual bats suggests that the highly ordered networks presumed to support spatial navigation in two dimensions cannot be routinely established in three-dimensional space.
Inactivation of the Dictyostelium orthologue of the tumour suppressor Neurofibromin (NF1) enables amoebae to ingest dissolved nutrients using macropinocytosis more rapidly, and to prey on larger organisms using phagocytosis.
Homologous recombination is the default response to replication impedance by the RTS1 barrier, leading to restarted replication forks that suffer frequent collapse, triggering further rounds of recombination downstream of the barrier.
The PBAF chromatin-remodelling complex is essential for the proliferation of melanocytes and melanoma cells and is recruited to critical regulatory elements by physical and functional interactions with MITF, a transcription factor and master regulator of melanoma.
Directed differentiation of stem cells can generate ventral-anterior foregut spheroids that can expand into three-dimensional lung organoids with striking structural, cellular and molecular similarities to the human fetal lung.
Drosophila has almost all transcription factor binding specificities available to humans; and human transcription factors with divergent specificities operate in cell types that are not found in fruit flies.
The pain mediator bradykinin was found to sensitize spinal motoneurons by a direct postsynaptic action, questioning the role of motoneurons in the assessment of hyperalgesia since the withdrawal motor reflex is commonly used as a surrogate pain model.
Systematic analysis of a broad spectrum of behavioral and physiological traits reveals novel relationships among complex traits, and resolves their underlying combination of genetic loci and molecular mechanisms.
A functional link between representative family members of the CLCA channel regulator family and TMEM16 channels suggests that these protein families may cooperate in influencing multiple homeostatic and disease physiologies.
Genomic evidence suggests that L-gulonolactone oxidase-the terminal enzyme in vitamin C synthesis, which has been repeatedly lost throughout animal evolution-was lost in plants and other photosynthetic eukaryotes following plastid acquisition.
A phylogeny of all major groups of flatworms based on hundreds of genes sheds new light the early evolution of this important metazoan phylum, with particular significance for the original of vertebrate parasitism.
Sensory neurons that monitor ambient oxygen control a cascade of responses across multiple layers of interneurons to switch the global state of the nematode C. elegans, reprogramming behavior and gene expression to enable escape from or adaptation to surface exposure.
Mapping microbial landscapes in indoor environments can predict how contaminants and spoilage resistance genes propagate within food-production environments, yielding novel insight for controlling spoilage.
An attenuated Herpes simplex type 2 virus deleted in glycoprotein D can be used as an effective vaccine to provide robust transferable humoral immunity and complete protection in murine intravaginal and skin infection models.
The Ran GTPase plays a role in defining the physical properties of the nuclear pore complex transport channel by remodeling the binding interactions of importin-β with the nucleoporin Nup153 at the nuclear face of the pore.
Single molecule imaging reveals how the molecular motor myosin 5 walks in a compass-like spinning motion along its actin track resulting in efficient, robust and unidirectional motion on the nanoscale.
Stimulation of specific neurons within the hypothalamus triggers a fear-like emotion state and behavioral response in mice, challenging the textbook view that the hypothalamus serves simply as a relay for emotion states encoded in the amygdala.
Use of a newly developed experimental model in fruit flies reveals that death following traumatic brain injury is largely due to a mechanism by which brain damage triggers disruption of the intestinal barrier, leading to elevated levels of glucose in the circulatory system with deleterious consequences.
The inhibition of sterol-accelerated degradation of HMG CoA reductase by the vitamin K2 synthetic enzyme UBIAD1 may contribute to the accumulation of cholesterol that is associated with Schnyder corneal dystrophy.
A cell-surface receptor called Gpr52 is able to lower the levels of the disease-causing protein mutant huntingtin and suppress its toxicity when knocked-down, making this receptor a promising drug target in Huntington's disease.
Rapid yet comprehensive, genome-resolved analysis of fecal samples from premature infants reveals little overlap in strain composition of gut microbial communities, despite co-hospitalization, and suggests the existence of barriers to strain dispersal.